[Morphological and histochemical reactions of an organism to the administration of Clostridium botulinum toxin. IX. Reactions of the neurons of the abducens nerve nucleus and of the Darkshevich's nucleus to the administration of C. botulinum type B toxin]. / Morfologicheskaia i gistokhimicheskaia kharakteristika reaktsii organizma na vvedenie toksina Clostridium botulinum. Soobshchenie IX. Reaktsiia neironov iadra otvodiashchego nerva i iadra Darkshevicha na vvedenie toksina C. botulinum tipa B.

Pathomorphological and histochemical study carried out in guinea pigs at the stage of paralysis of the limbs after the oral administration of C. botulinum toxin has revealed the presence of morpho-metabolic changes pertaining to phase 2 of the development of dystrophic processes in the neurons of the nucleus of the abducent nerve and Darkshevich's nucleus. At the myasthenic stage the development of dystrophic processes in the neurons of Darkshevich's nucleus has been found to reach phase 3. The study indicates that C. botulinum toxin possesses different tropism in respect of the neurons of the oculomotor nuclei; this fact correlates with the clinical picture of botulism.

[Morphologic and histochemical responses of the body to introduction of C1. botulinum toxin. V. Response of locus coeruleus cells of the brain to introduction of the toxin of C1. botulinum type B]. / Morfologicheskaia i gistokhimicheskaia kharakteristika reaktsii organizma na vvedenie toksina C1. botulinum. Soobshchenie V. Reaktsiia kletok sinego piatna (locus coeruleus) mozga na vvedenie toksina C1. botulinum tipa B.

After the per os administration of 1Dlm/ml of botulin, type B. there were observed at the period of development of the limb paralysis and myasthenia morphological changes in the locus coeruleus cells expressed in the development of a more pronounced than normal perinuclear clarification and some nuclear basophilia. Histochemical study of succinic dehydrogenase, acid phosphatase, cholinesterase, and catecholamines pointed to the intensified neuron metabolism of the locus coeruleus; this could serve as an indication of enhanced energopacemaker function of this nucleus in hypoxia which occurred.

[Morphological and histochemical characteristics of the organism's reaction to administration of Cl botulinum toxin. IV. Cytochemical changes in the cells of the mesencephalic nucleus of trigeminal nerve following administration of Cl. botulinum type B toxin]. / Morfologicheskaiai gistokhimicheskaia kharakteristika reaktsii organizma na vvedenie tosina Cl. botulinum. Soobshchenie IV. Tsitokhimicheskie izmenenia v kletkakh mezentsefalicheskogo iadra troinichnogo nerva posle vvedeniia toksina Cl. botulinum tipa B

After the administration to guinea pigs per os of 1 Dlm of botulin toxin, type B, a change of the RNA synthesis in the nucleoli and DNA depolymerization in the nuclei was observed in some of the cells of the mesencephalic nucleus of the trigeminal nerve. An increase in the activity of succinic dehydrogenase and of acid phosphatase coursed without any necrotic processes in the cells. Basing upon the changes in the activity of succinic dehydrogenase in the neurons of the mesencephalic nucleus a conclusion was drawn that hypoxia began to develop at the period of appearance of paralyses of the limbs and reached its maximum in myasthenia.

[Morphological and histochemical characteristics of the reaction of the body to Cl. botulinum toxin administration. III. The cellular reaction of the diaphragmatic nerve nucleus to the administration of Cl. botulinum type B toxin]. / Morfologicheskaia i gistokhimicheskaia kharakteristika reaktsii organizma na vvedenie toksina C. botulinum. Soobshchenie III. Reaktsiia kletok iadra diafragmal'nogo nerva na vvedenie toksina Cl. botulinum tipa B

On the appearance in the animals (guinea pigs) of paralysis of the limbs and myasthenia after the administration of Cl. botulinum, type B, toxin, there was seen a considerable vascular hyperemia of the spinal cord, and in the neurons of the phrenic nerve nucleus there developed dystrophic-necrotic processes coursing with a marked swelling, hyperchromasia and tigrolysis. As revealed histochemically, at this stage of the botulin intoxication the neurons of the phrenic nerve nucleus displayed metabolic changes expressed in the altered activity of succinic dehydrogenase, acid phosphatase and cholinesterase.