ABSTRACT
BACKGROUND: Some studies reported that differential gene expression could be used as a biomarker for high-grade cervical lesion identification. The aim was to evaluate the gene expression profile of cervical intraepithelial neoplasia (CIN) to identify a gene expression signature of CIN2+ in liquid-based cytology (LBC) samples. METHODS: LBC samples (n = 85) obtained from women who underwent colposcopy were included with benign (n = 13), CIN1 (n = 26), CIN2 (n = 16), and CIN3 (n = 30) diagnoses. After RNA isolation, gene expression profiling was performed using the nCounter PanCancer Pathways, which consists of 730 cancer-related genes. The genes identified were in silico expression evaluated using the UALCAN database. An accurate prediction model to discriminate CIN2+ from Subject(s)
Papillomavirus Infections
, Uterine Cervical Dysplasia
, Uterine Cervical Neoplasms
, Pregnancy
, Humans
, Female
, Uterine Cervical Neoplasms/diagnosis
, Uterine Cervical Neoplasms/genetics
, Uterine Cervical Neoplasms/metabolism
, Cytology
, Cervix Uteri/pathology
, Uterine Cervical Dysplasia/diagnosis
, Cytodiagnosis
, Colposcopy
, Papillomavirus Infections/diagnosis
, Papillomaviridae/genetics
, Bone Morphogenetic Protein 7
, Membrane Proteins
, Protein-Tyrosine Kinases
, Protein Serine-Threonine Kinases
ABSTRACT
Colorectal cancer (CRC) has a high incidence and mortality worldwide. Microsatellite instability (MSI) is crucial in CRC, with distinct molecular and clinicopathological features in patients. Nowadays, it is a predictive marker for immunotherapy. We proposed to evaluate the 5-year outcome of MSI status in 1002 Brazilian CRC, and associate it with genetic ancestry, molecular and clinicopathological features. MSI evaluation was performed using molecular markers. MSI+ tumors were analyzed for alterations in 23 MSI-targeted genes. Genetic ancestry was evaluated using an Ancestry-Informative markers panel. MSI status was analyzed in relation to CRC specific survival and other clinical and genetic variables. MSI+ status was observed in 10.5% of cases. MSI+ status was significantly associated with the anatomic site right colon, mucinous histological type, clinical stage II, histological grade III/undifferentiated, no recurrence of disease, and live cases without cancer. No association of MSI status with genetic ancestry components was observed. MSI-targeted genes analyses showed the most frequently altered genes: ATM, EGFR, MRE11, ROCK1, and TGFBRII. There was a statistically significant difference in cancer-specific survival between cases according to MSI status. This study constitutes the most comprehensive analyses of the MSI impact on the Brazilian CRC. MSI+ frequency in Brazilian CRC agreed with the literature and was associated with several clinicopathological features related with less aggressive tumors, independently of their genetic ancestry.
Subject(s)
Colorectal Neoplasms , Microsatellite Instability , Brazil , Colorectal Neoplasms/pathology , Humans , Microsatellite Repeats , Prognosis , rho-Associated Kinases/genetics , rho-Associated Kinases/therapeutic useABSTRACT
Colorectal cancer (CRC) is a leading cause of cancer death worldwide. Microsatellite instability (MSI) is a genetic pathway leading to CRC, associated with particular clinicopathological features, and recently a major biomarker of immunotherapy response. There is little information the frequency MSI among Brazilian CRC patients, and it is still debatable the ideal methodology for MSI screening in countries with limited resources. We proposed to evaluate MSI by molecular and immunohistochemistry (IHC) methods, to compare both methodologies and also to assess the inclusion of a novel microsatellite marker, HSP110 (T17). The molecular MSI evaluation was performed using a PCR-multiplex panel in a total of 1013 CRC patients. Mismatch repair (MMR) proteins (MLH1, MSH2, MSH6 and PMS2) expression were evaluated by IHC. HSP110 (T17) marker was analyzed by fragment analysis. Molecularly, 89.5% of cases were MSI-negative and 10.5% were MSI-positive. The IHC showed that 88.9% of cases exhibited MMR-proficient status, 10.2% were MMR-deficient and 0.9% was inconclusive. Genotyping of the HSP110 (T17) in 106 MSI-positive and 215 MSI-negative cases showed its alteration only among the MSI-positive cases. We observed agreement (0.956, Kappa Test) between both molecular and IHC methodologies, with only eight discordant results, and in this subset of cases the HSP110 (T17) corroborate the molecular findings. This study suggests the use of molecular assays over IHC for MSI analysis and proposes the inclusion HSP110 (T17) marker as a complementary analysis in discordant cases.
ABSTRACT
Nutrition value of silage from corn hybrids produced in Mato Grosso, State was evaluated. A randomized block design was used with 23 treatments (hybrids) and three replications. The study used hybrids from different seed companies. Fodder was stored in PVC pipes at a density of 600 kg of green mass m-³. The silos were opened 90 days after ensiling, and the following variables were studied: pH, dry matter (DM), ammoniacal nitrogen (N-NH3), crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), total digestible nutrients (TDN) and minerals (Ca, P, K and Mg). Rates were estimated for dry matter intake (DMI), dry matter digestibility (DM), net energy for maintenance (NEm), gain (NEg) and lactation (EL). All the characteristics were determined by near-infrared spectroscopy (NIR). With regard to standard fermentation, silage of different hybrids had appropriate values for pH and N-NH3. The silage of hybrids DKB 370, DKB 330, DAS 2C520, DAS 2B710, DAS 2B587, BF 9534, AG 9010, AG 8088, AG 5020, BE 9701, AGN 30A06 e AGN 31A31 showed lower NDF and higher estimated DMI values
ABSTRACT
Nutrition value of silage from corn hybrids produced in Mato Grosso, State was evaluated. A randomized block design was used with 23 treatments (hybrids) and three replications. The study used hybrids from different seed companies. Fodder was stored in PVC pipes at a density of 600 kg of green mass m-³. The silos were opened 90 days after ensiling, and the following variables were studied: pH, dry matter (DM), ammoniacal nitrogen (N-NH3), crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), total digestible nutrients (TDN) and minerals (Ca, P, K and Mg). Rates were estimated for dry matter intake (DMI), dry matter digestibility (DM), net energy for maintenance (NEm), gain (NEg) and lactation (EL). All the characteristics were determined by near-infrared spectroscopy (NIR). With regard to standard fermentation, silage of different hybrids had appropriate values for pH and N-NH3. The silage of hybrids DKB 370, DKB 330, DAS 2C520, DAS 2B710, DAS 2B587, BF 9534, AG 9010, AG 8088, AG 5020, BE 9701, AGN 30A06 e AGN 31A31 showed lower NDF and higher estimated DMI values
ABSTRACT
The experiment was carried out in Cáceres, Mato Grosso State, Brazil, aiming to evaluate the effect of nitrogen (N) on forage production and chemical composition of Tifton 85 bermudagrass (Cynodon spp.). A randomized complete block design was used, with five treatments (0, 60, 120, 180 and 240 kg ha-1) and four replications in 4.0 x 5.0 m plots. Four cuts were made at intervals of 30 days, at the height of 5 cm above ground level. N was applied after each cut, in the form of urea. For each kg ha-1 of N applied, we observed increases of 22.67 kg ha-1 in the production of total dry mass, 11.37 kg ha-1 in the production of green leaf blade, 0.052 cm in sward height, and 0.00955 dag kg-1 in crude protein content, and a reduction of 0.0142 dag kg-1 in NDF content. There was an increase in the extraction of N at a rate of 0.537 kg kg-1 of N, and sharp decline in the efficiency of N utilization from the estimated dose of 155 kg ha-1 of N.
O experimento foi conduzido em Cáceres, Estado do Mato Grosso, com o objetivo de avaliar o efeito de doses de nitrogênio sobre a produção de forragem e a composição química do capim-tifton 85 (Cynodon spp.). Utilizou-se o delineamento em blocos completos casualizados com cinco tratamentos (0, 60, 120, 180 e 240 kg há-1 de N) e quatro repetições, em parcelas de 4,0 x 5,0 m. Foram realizados quatro cortes a intervalos de 30 dias, na altura de 5 cm do nível do solo, sendo as doses de N parceladas em quatro aplicações após cada corte, sob a forma de ureia, em cobertura. Foi observado, para cada kg ha-1 de N aplicado, aumento, na produção de matéria seca total de 22,67 kg ha-1, na produção de lâmina foliar verde de 11,37 kg ha-1, na altura do relvado de 0,052 cm e no teor de proteína bruta da forragem de 0,00955 dag kg-1, e diminuição no teor de FDN de 0,0142 dag kg-1. Observou-se aumento na extração de N a uma taxa de 0,537 kg kg-1 de N e diminuição acentuada da eficiência de utilização do N a partir da dose estimada de 155 kg ha-1 de N.
ABSTRACT
The experiment was carried out in Cáceres, Mato Grosso State, Brazil, aiming to evaluate the effect of nitrogen (N) on forage production and chemical composition of Tifton 85 bermudagrass (Cynodon spp.). A randomized complete block design was used, with five treatments (0, 60, 120, 180 and 240 kg ha-1) and four replications in 4.0 x 5.0 m plots. Four cuts were made at intervals of 30 days, at the height of 5 cm above ground level. N was applied after each cut, in the form of urea. For each kg ha-1 of N applied, we observed increases of 22.67 kg ha-1 in the production of total dry mass, 11.37 kg ha-1 in the production of green leaf blade, 0.052 cm in sward height, and 0.00955 dag kg-1 in crude protein content, and a reduction of 0.0142 dag kg-1 in NDF content. There was an increase in the extraction of N at a rate of 0.537 kg kg-1 of N, and sharp decline in the efficiency of N utilization from the estimated dose of 155 kg ha-1 of N.
O experimento foi conduzido em Cáceres, Estado do Mato Grosso, com o objetivo de avaliar o efeito de doses de nitrogênio sobre a produção de forragem e a composição química do capim-tifton 85 (Cynodon spp.). Utilizou-se o delineamento em blocos completos casualizados com cinco tratamentos (0, 60, 120, 180 e 240 kg há-1 de N) e quatro repetições, em parcelas de 4,0 x 5,0 m. Foram realizados quatro cortes a intervalos de 30 dias, na altura de 5 cm do nível do solo, sendo as doses de N parceladas em quatro aplicações após cada corte, sob a forma de ureia, em cobertura. Foi observado, para cada kg ha-1 de N aplicado, aumento, na produção de matéria seca total de 22,67 kg ha-1, na produção de lâmina foliar verde de 11,37 kg ha-1, na altura do relvado de 0,052 cm e no teor de proteína bruta da forragem de 0,00955 dag kg-1, e diminuição no teor de FDN de 0,0142 dag kg-1. Observou-se aumento na extração de N a uma taxa de 0,537 kg kg-1 de N e diminuição acentuada da eficiência de utilização do N a partir da dose estimada de 155 kg ha-1 de N.
ABSTRACT
The objective of this study was to evaluate the thermal environment in growing and finishing swine housing and by using the temperature and humidity index (THI) and black globe temperature and humidity index (BGHI), under tropical conditions. The dry bulb temperature, relative humidity, wind velocity, THI, BGHI and the radiant thermal load index (RTL) were measured inside and outside the housing. The mean of BGHI inside the housing during summer and winter station had values between 68.9 to 74.8 and 55.3 to 61.2 and the results showed that during the hottest periods of the day the housing provided lower BGHI (P 0.05). The THI was not accurate for determining thermal comfort under tropical conditions. The RTL was reduced up to 35% inside the housing due to the shade effect.
Neste trabalho, avaliou-se o ambiente térmico de uma instalação de crescimento e terminação de suínos e compararam-se os índices de temperatura e umidade (ITU) e de globo negro e umidade (ITGU) na indicação do conforto térmico nas condições tropicais. Foram medidas a temperatura de bulbo seco, a umidade relativa do ar e a velocidade do vento, e determinados os índices de temperatura e umidade, de globo negro e umidade e da carga térmica radiante (CTR). As mesmas análises foram realizadas para o ambiente externo (área não sombreada). As médias de ITGUs pela instalação, no verão e inverno, ficaram entre 68,9 a 74,8 e de 55,3 a 61,2, respectivamente, e foram observadas diferenças (P 0,05) em relação à área não sombreada principalmente nos períodos mais quentes do dia. O ITGU foi mais adequado na indicação do conforto térmico em relação ao ITU, principalmente no verão. A redução da CTR em função da instalação foi de até 35% quando comparado com a área não sombreada.
ABSTRACT
The objective of this study was to evaluate the thermal environment in growing and finishing swine housing and by using the temperature and humidity index (THI) and black globe temperature and humidity index (BGHI), under tropical conditions. The dry bulb temperature, relative humidity, wind velocity, THI, BGHI and the radiant thermal load index (RTL) were measured inside and outside the housing. The mean of BGHI inside the housing during summer and winter station had values between 68.9 to 74.8 and 55.3 to 61.2 and the results showed that during the hottest periods of the day the housing provided lower BGHI (P 0.05). The THI was not accurate for determining thermal comfort under tropical conditions. The RTL was reduced up to 35% inside the housing due to the shade effect.
Neste trabalho, avaliou-se o ambiente térmico de uma instalação de crescimento e terminação de suínos e compararam-se os índices de temperatura e umidade (ITU) e de globo negro e umidade (ITGU) na indicação do conforto térmico nas condições tropicais. Foram medidas a temperatura de bulbo seco, a umidade relativa do ar e a velocidade do vento, e determinados os índices de temperatura e umidade, de globo negro e umidade e da carga térmica radiante (CTR). As mesmas análises foram realizadas para o ambiente externo (área não sombreada). As médias de ITGUs pela instalação, no verão e inverno, ficaram entre 68,9 a 74,8 e de 55,3 a 61,2, respectivamente, e foram observadas diferenças (P 0,05) em relação à área não sombreada principalmente nos períodos mais quentes do dia. O ITGU foi mais adequado na indicação do conforto térmico em relação ao ITU, principalmente no verão. A redução da CTR em função da instalação foi de até 35% quando comparado com a área não sombreada.
ABSTRACT
This study was developed in urograndis eucalypt hibrid (Eucalyptus urophyllaX Eucalyptus grandis)plantation belonging to Jari Celulose S.A., in Monte Dourado, Pará State, Brazil, from July 1993 to January 1994. Mirex-S (sulfluramid 0.3%) bait at the dosages of 6, 8 and 10 grams was compared to 10 grams of a dodecachlor (0.45%) bait per square meter of ant hill, against Atta cephalotes(Hymenoptera: Formicidae). At the dosages of 6, 8 and 10 grams the Mirex-S bait showed 72.7; 83.3 and 91.67% of nest control compared to 87.5% for the dodecachlor bait. Therefore, Mirex-S bait at 8 and 10 grams per square meter of ant hill can replace the dodecachlor baits for A. cephalotescontrol.
Este trabalho foi realizado em reflorestamentos de eucalipto híbrido urograndis (Eucalyptus urophyllaX Eucalyptus grandis)da Jari Celulose S.A, no município de Almerim, Pará, de julho de 1993 a janeiro de 1994. Objetivou-se testar a eficiência da isca granulada Mirex-S (sulfluramida 0,3%), nas dosagens de 6, 8 e 10 gramas por metro quadrado de formigueiro, em comparação com 10 gramas de uma isca a base de dodecacloro (0,45%) para o controle de Atta cephalotes(Hymenoptera: Formicidae). A isca Mirex-S apresentou índices de controle, respectivamente, de 72,70%, 83,33% e 91,67%, em contrapartida com 87,50% da isca a base de dodecacloro. A isca Mirex-S, a 8 e 10 gramas por metro quadrado de formigueiro, pode substituir com eficiência as iscas à base de dodecacloro para o controle de A. cephalotes.
ABSTRACT
This study was developed in urograndis eucalypt hibrid (Eucalyptus urophyllaX Eucalyptus grandis)plantation belonging to Jari Celulose S.A., in Monte Dourado, Pará State, Brazil, from July 1993 to January 1994. Mirex-S (sulfluramid 0.3%) bait at the dosages of 6, 8 and 10 grams was compared to 10 grams of a dodecachlor (0.45%) bait per square meter of ant hill, against Atta cephalotes(Hymenoptera: Formicidae). At the dosages of 6, 8 and 10 grams the Mirex-S bait showed 72.7; 83.3 and 91.67% of nest control compared to 87.5% for the dodecachlor bait. Therefore, Mirex-S bait at 8 and 10 grams per square meter of ant hill can replace the dodecachlor baits for A. cephalotescontrol.
Este trabalho foi realizado em reflorestamentos de eucalipto híbrido urograndis (Eucalyptus urophyllaX Eucalyptus grandis)da Jari Celulose S.A, no município de Almerim, Pará, de julho de 1993 a janeiro de 1994. Objetivou-se testar a eficiência da isca granulada Mirex-S (sulfluramida 0,3%), nas dosagens de 6, 8 e 10 gramas por metro quadrado de formigueiro, em comparação com 10 gramas de uma isca a base de dodecacloro (0,45%) para o controle de Atta cephalotes(Hymenoptera: Formicidae). A isca Mirex-S apresentou índices de controle, respectivamente, de 72,70%, 83,33% e 91,67%, em contrapartida com 87,50% da isca a base de dodecacloro. A isca Mirex-S, a 8 e 10 gramas por metro quadrado de formigueiro, pode substituir com eficiência as iscas à base de dodecacloro para o controle de A. cephalotes.
ABSTRACT
This survey of the ant fauna in a native forest and in three plantations of Eucalyptus of 6, 20 and 106 months of age was conducted in August 1992. Sampling consisted of two 100 m transects per area along which 10 pitfall traps were placed for 7 days, after which they were replaced for traps baits with sardines, crackers and sugar laid during one hour per day. In addition hand collecting was performed on soil, litter, trees and shrubs for five non consecutive hours in each area. A total of 121 species belonging to 5 subfamilies and 50 genera was collected. The calculated Hill indices have indicated that in the native area there was a lower dominance and a higher species diversity. In the eucalipt there was a tendency towards reduced dominance and increased diversity as the stands grew older.
Um levantamento da fauna de formigas em uma área de mata nativa e em três plantações de Eucalyptus de 6, 20 e 106 meses de idade foi conduzido em agosto de 1992. Aamostragem consistiu de 2 transectos de 100 m por área, sendo que ao longo de cada transecto foram dispostas 10 armadilhas pitfall, que permaneceram no campo por 7 dias, após o que, foram substituídas por armadilhas contendo iscas de bolacha, sardinha e açúcar, que permaneceram na área por 1 hora para coleta das formigas. Além disso, para cada área foram feitas coletas manuais sobre o solo, senapilheira, árvores e arbustos por 5 horas não consecutivas.Um total de 121 morfoespécies, distribuídas em 5 subfamílias e 50 gêneros foram coletados. Os índices de Hill calculados indicam que na mata nativa houve uma baixa dominância e alta diversidade de espécies, enquanto nos plantios de eucalipto observou-se uma tendência de diminuição da dominância e aumento na diversidade com o crescimento da floresta.
ABSTRACT
This survey of the ant fauna in a native forest and in three plantations of Eucalyptus of 6, 20 and 106 months of age was conducted in August 1992. Sampling consisted of two 100 m transects per area along which 10 pitfall traps were placed for 7 days, after which they were replaced for traps baits with sardines, crackers and sugar laid during one hour per day. In addition hand collecting was performed on soil, litter, trees and shrubs for five non consecutive hours in each area. A total of 121 species belonging to 5 subfamilies and 50 genera was collected. The calculated Hill indices have indicated that in the native area there was a lower dominance and a higher species diversity. In the eucalipt there was a tendency towards reduced dominance and increased diversity as the stands grew older.
Um levantamento da fauna de formigas em uma área de mata nativa e em três plantações de Eucalyptus de 6, 20 e 106 meses de idade foi conduzido em agosto de 1992. Aamostragem consistiu de 2 transectos de 100 m por área, sendo que ao longo de cada transecto foram dispostas 10 armadilhas pitfall, que permaneceram no campo por 7 dias, após o que, foram substituídas por armadilhas contendo iscas de bolacha, sardinha e açúcar, que permaneceram na área por 1 hora para coleta das formigas. Além disso, para cada área foram feitas coletas manuais sobre o solo, senapilheira, árvores e arbustos por 5 horas não consecutivas.Um total de 121 morfoespécies, distribuídas em 5 subfamílias e 50 gêneros foram coletados. Os índices de Hill calculados indicam que na mata nativa houve uma baixa dominância e alta diversidade de espécies, enquanto nos plantios de eucalipto observou-se uma tendência de diminuição da dominância e aumento na diversidade com o crescimento da floresta.
ABSTRACT
Mineral nutrition of Siratro (Macroptilium atropurpureum) was studied in a greenhouse experiment held at the Estação Experimental Central de Nova Odessa. SP. Brazi1 of the Instituto de Zooteena, from September to November, 1978, ,-with three fertilization levels: I control (without fertilization); II with phosphorus, calcium and sulphur; III - treatment II plus liming, potassium, zinc and molybdenum, using four different soils of São Paulo State (two Latosols. one Podzol and one Ouartzous Sand), all of them with low fertility. The dry matter production and height of me plants were significantly increased with fertilization tratments applied to all soils, except for the soils Latosol ano, from Itapetininga. which had low plant productions in all treatments. Dry matter production in the soils LVA, terraço and Ouartzous Sand was greater with treatment III than with treatment II and for the Red-Yellow Podzolic soil Laras variation there was no statistical differences beetween the trealments II and III.
Em ensaio de vasos, realizado em casa de vegetação na Estação Experimental de Nova Odessa (SP), estudou-se a resposta do siratro (Macroptilium Atropurpureum cv. Siratro) a três tratamentos de fertilização: tratamento I: testemunha sem adubação; tratamento II; fósforo, cálcio e enxofre; tratamento III, tratamento II mais calagem, potássio, zinco e molibdênio, em quatro solos do Estado de São Paulo (Latosol Vermelho- -Amarelo fase terraço, série Pinda; Podzolico Vermelho-Amarelo variação Laras; Latool Vermelho-Escuro Orto e Areia Ouartzosa As respostas ás fertilizações em termos de produção de matéria seca e altura das plantas foram significativas para todos os solos, com exceção do Latosol Vermelho-Escuro Orto, coletado no município de Itapetininga. o qual apresentou baixa produtividade das plantas em todos os tratamentos. A produção de matéria seca nos solos LVA fase terraço e Areia Ouartzosa foi significativamente maior com a fertilização do tratamento III do que com a do tratamento II, e para o Podzólico Vermelho- -Amarelo variação Laras não houve diferenças entre os tratamentos II e III.
ABSTRACT
Mineral nutrition of Siratro (Macroptilium atropurpureum) was studied in a greenhouse experiment held at the Estação Experimental Central de Nova Odessa. SP. Brazi1 of the Instituto de Zooteena, from September to November, 1978, ,-with three fertilization levels: I control (without fertilization); II with phosphorus, calcium and sulphur; III - treatment II plus liming, potassium, zinc and molybdenum, using four different soils of São Paulo State (two Latosols. one Podzol and one Ouartzous Sand), all of them with low fertility. The dry matter production and height of me plants were significantly increased with fertilization tratments applied to all soils, except for the soils Latosol ano, from Itapetininga. which had low plant productions in all treatments. Dry matter production in the soils LVA, terraço and Ouartzous Sand was greater with treatment III than with treatment II and for the Red-Yellow Podzolic soil Laras variation there was no statistical differences beetween the trealments II and III.
Em ensaio de vasos, realizado em casa de vegetação na Estação Experimental de Nova Odessa (SP), estudou-se a resposta do siratro (Macroptilium Atropurpureum cv. Siratro) a três tratamentos de fertilização: tratamento I: testemunha sem adubação; tratamento II; fósforo, cálcio e enxofre; tratamento III, tratamento II mais calagem, potássio, zinco e molibdênio, em quatro solos do Estado de São Paulo (Latosol Vermelho- -Amarelo fase terraço, série Pinda; Podzolico Vermelho-Amarelo variação Laras; Latool Vermelho-Escuro Orto e Areia Ouartzosa As respostas ás fertilizações em termos de produção de matéria seca e altura das plantas foram significativas para todos os solos, com exceção do Latosol Vermelho-Escuro Orto, coletado no município de Itapetininga. o qual apresentou baixa produtividade das plantas em todos os tratamentos. A produção de matéria seca nos solos LVA fase terraço e Areia Ouartzosa foi significativamente maior com a fertilização do tratamento III do que com a do tratamento II, e para o Podzólico Vermelho- -Amarelo variação Laras não houve diferenças entre os tratamentos II e III.