ABSTRACT
Objectives: Among the diverse forms of amyloidosis, secondary type is the most frequent one. Diagnosis of amyloiddeposition is based on the identification of the fibrillary protein amyloid by means of Congo Red (CR) or crystalviolet (CV) stains, but these techniques do not differentiate between the different types of amyloid fibrils. Theaim of this study was to identify by immunofluorescence (IF) AA amyloid a pathological fibrillar low-molecularweightprotein formed by cleavage of serum amyloid A (SAA) protein in labial salivary gland (LSG) biopsies frompatients with secondary amyloidosis.Study design: 98 LSG were studied, 65 were from patients with secondary amyloidosis and 33 from subjects withchronic inflammatory diseases without evidence of this anomaly. All sections were stained with hematoxylin andeosin (H&E), CV, CR and IF using anti-AA antibodies. Positive and negative controls were used for all techniques.Results: CV and CR demonstrated that the amyloid substance was found mainly distributed periductally (93.8%),followed by periacinar and perivascular locations (p<0.001); however, the IF demonstrated that amyloid AA substancepredominates in the periacinar area (73.8%), followed by periductal and perivascular locations (p<0.001).IF has a sensitivity of 83%, 100% of specificity, 100% of predictive positive value and 75% of predictive negativevalue.Conclusions: The results of this study confirm the efficacy of the LSG biopsy as a highly reliable method fordiagnosis of secondary amyloidosis (AU)
Subject(s)
Humans , Serum Amyloid A Protein/isolation & purification , Salivary Glands , Amyloidosis/diagnosis , Congo RedABSTRACT
OBJECTIVES: Among the diverse forms of amyloidosis, secondary type is the most frequent one. Diagnosis of amyloid deposition is based on the identification of the fibrillary protein amyloid by means of Congo Red (CR) or crystal violet (CV) stains, but these techniques do not differentiate between the different types of amyloid fibrils. The aim of this study was to identify by immunofluorescence (IF) AA amyloid a pathological fibrillar low-molecular-weight protein formed by cleavage of serum amyloid A (SAA) protein in labial salivary gland (LSG) biopsies from patients with secondary amyloidosis. STUDY DESIGN: 98 LSG were studied, 65 were from patients with secondary amyloidosis and 33 from subjects with chronic inflammatory diseases without evidence of this anomaly. All sections were stained with hematoxylin and eosin (H &E), CV, CR and IF using anti-AA antibodies. Positive and negative controls were used for all techniques. RESULTS: CV and CR demonstrated that the amyloid substance was found mainly distributed periductally (93.8%), followed by periacinar and perivascular locations (p <0.001); however, the IF demonstrated that amyloid AA substance predominates in the periacinar area (73.8%), followed by periductal and perivascular locations (p <0.001). IF has a sensitivity of 83%, 100% of specificity, 100% of predictive positive value and 75% of predictive negative value. CONCLUSIONS: The results of this study confirm the efficacy of the LSG biopsy as a highly reliable method for diagnosis of secondary amyloidosis.
Subject(s)
Amyloidosis/pathology , Salivary Gland Diseases/pathology , Salivary Glands, Minor/chemistry , Serum Amyloid A Protein/analysis , Adolescent , Adult , Aged , Aged, 80 and over , Amyloidosis/metabolism , Female , Fluorescent Antibody Technique , Humans , Lip , Male , Middle Aged , Salivary Gland Diseases/metabolism , Sensitivity and Specificity , Serum Amyloid A Protein/biosynthesis , Young AdultABSTRACT
47 biopsias hepáticas con diagnóstico anatomopatológico de cirrosis fueron procesados por inmunoperoxidasa para determinar la presencia de HBsAg. Este método muy sensible y específico tiene aún poca difusión en nuestro medio. Se clasificó las biopsias en dos grupos: cirrosis hepática postnecrótica (PN) (37) y cirrosis hepática no postnecrótica (NP) (10). Nueve tejidos resultados positivos, representan el 19.15 por ciento del total de nuestra población y pertenecen al grupo de cirrosis hepática postnecrótica. Esta técnica es propuesta como método auxiliar en casos clínicos con problema diagnóstico, pacientes con infección por HBV, y principalmente en tejidos provenientes de zonas endémicas.
Subject(s)
Humans , Biopsy , Hepatitis B Surface Antigens , Liver Cirrhosis , Immunoenzyme TechniquesABSTRACT
47 liver biopsies with anatomopathologial diagnosis of cirrhosis were processed by immunoperoxidase (IP) to determine the presence of HBsAg. This highly sensible and specific method has been very seldom used in our media before.Biopsies were classified into two groups: postnecrotic hepatic cirrosis (PN) (37) and non postnecrotic hepatic cirrhosis (NP) (10). Nine biopsies were IP positive and represent 19.15% from the whole population and all of them belong to PN hepatic cirrhosis.This technique is proposed as an auxiliary method when confronting diagnostic problems, and specially in tissues of endemic zones.
