ABSTRACT
Recently, we demonstrated for the first time liquid-phase microextraction (LPME) of polar drugs based on carrier mediated transport. In this new extraction technique, selected analytes were extracted as ion-pairs from small volumes of biological samples, through a thin layer of a water immiscible organic solvent immobilised in the pores of a porous hollow fibre (liquid membrane), and into a microl volume of an acidic aqueous acceptor solution placed inside the lumen of the hollow fibre. In the current paper, this new extraction technique was combined with liquid chromatography-mass spectrometry (LC-MS) for the first time. Carrier mediated LPME was evaluated for several new model drugs (0.01 Subject(s)
Chemical Fractionation/methods
, Chromatography, Liquid/methods
, Microchemistry/methods
, Pharmaceutical Preparations/blood
, Amphetamine/blood
, Amphetamine/isolation & purification
, Atenolol/blood
, Atenolol/isolation & purification
, Cimetidine/blood
, Cimetidine/isolation & purification
, Humans
, Morphine/blood
, Morphine/isolation & purification
, Phenylpropanolamine/blood
, Phenylpropanolamine/isolation & purification
, Practolol/blood
, Practolol/isolation & purification
, Sensitivity and Specificity
, Solvents
, Sotalol/blood
, Sotalol/isolation & purification
, Spectrometry, Mass, Electrospray Ionization/methods