Baseline Haematology and Erythrocyte Morphological Changes of Apparently Normal Dogs Raised in Ibadan, Oyo State.

This study evaluates the haematological parameters and the observed erythrocytes morphological changesin dogs raised in Ibadan, Oyo State in the south western part of Nigeria. Blood samples were collected from sixty-four apparently healthy dogs. The haematological parameters of the blood samples collected were evaluated with the quantification of the percentage erythrocyte morphological abnormalities. The result of the quantitative count of the erythrocyte morphological abnormalities were also converted using reference guide to give the numerical/descriptive clinical grade of the associated morphological abnormalities. There was a significant difference between the haematological parameters reported in this study and the commonly used dog haematological reference ranges from temperate regions used in laboratories in Nigeria. Some of the morphological abnormalities observed in this study include echinocyte (4.12±0.35%), macrocyte (2.61±0.22%) and spherocyte (2.17±0.29%) and eccentrocyte (0.39±0.06%) while other such as acanthocyte (1.39±0.19), leptocyte (0.71±0.13), schizocyte (0.703±0.104) and codocyte (0.50±0.11) were also seen. These study findings show that the associated morphological changes were all not significant and fall within the acceptable range using the reference guide for erythrocyte morphological abnormalities even when the haematological values differ significantly fromthe reference value. This study provides baseline information on the haematological parameters and the novel correlation ofthe associated erythrocyte abnormalities seen as a corresponding proof of the apparently healthy status of the dogs raised in Ibadan used for this study. The study while serving as an important means of verification of the reference range of haematological parameters also shows that clinical case interpretation using haematological baseline data from the temperate region should be used with caution in our tropical environment. This thus necessitates the need for an advocacy to build a reference range of haematological parameters that can be used as a working baseline value for the tropical environment.

Oviductal volvulus in a Nera black chicken (Gallus gallus domesticus) in Nigeria.

A case of oviductal volvulus, a rare disorder with oviductal cyst as a possible aetiology, is described in an 11-month-old Nera black chicken (Gallus gallus domesticus). The condition was observed during routine postmortem examination of 550 carcasses from a flock of 16,100 birds between September 2005 and June 2006. The affected portion of the oviduct and the cyst twisted almost 360 degrees clockwise around the dorsal ligament with severe congestion, hyperaemia, oedema, dilatation and devitalization. This is the first reported case of oviductal volvulus in a domestic chicken.

Trace element levels in plasma/serum and erythrocytes of Keteku and white Fulani cattle.

In order to determine the probable role of trace elements in the maintenance of trypanotolerance in tropical cattle, the levels of zinc (Zn), copper (Cu), manganese (Mn) and iron (Fe) in plasma/serum and erythrocytes of trypanotolerant Keteku (Kt) and trypanosusceptible White Fulani (WF) cattle were measured by atomic absorption spectrophotometry (AAS) and compared by breed or sex. Trypanotolerance was associated with significantly higher (P < 0.05) erythrocyte levels of Zn and Mn. The Kt breed showed higher erythrocyte levels of Zn and Mn than the WF. Serum Mn level was also significantly higher (P < 0.01) in Kt than WF cattle. However, PCV and total protein concentration were not significantly different by breed or sex.

Use of monoclonal antibodies for detecting T brucei brucei infection in splenectomised dogs.

A monoclonal antibody against a plasma membrane antigen of Trypanosoma rhodesiense was used for the detection of T brucei group-specific circulating antigen in 24 adult local dogs experimentally infected with T brucei brucei strain 8/18. Ten of the dogs were splenectomised and the remainder non-splenectomised (intact). Five dogs each from the splenectomised and intact groups were inoculated intravenously with trypanosomes. The infected dogs developed trypanosomiasis between days 4 and 8 after infection. The circulating antigens were detected as early as six days after infection and remained high until two weeks after treatment, when the circulating antigen declined. The detection of the antigens showed the existence of infection unlike the antibody test. The treatment of the infected dogs with diminazene aceturate (Berenil; Hoechst) at a dose of 7.0 mg/kg on day 21 after infection cleared all the parasites but elevated the circulating antigen levels. The antigen capture enzyme-linked immunosorbent assay is a useful diagnostic tool for complementing parasitological diagnosis, for detecting infection in the field and for ascertaining the efficacy of trypanocidal drugs.

The effect of trypanocidal drug treatment on the haematological changes in Trypanosoma brucei brucei infected splenectomised dogs.

Twenty adult mongrel dogs of both sexes were used. Ten of the dogs were splenectomised and the remaining dogs were left intact. Five dogs each from the splenectomised and non-splenectomised (intact) animals were infected intravenously with Trypanosoma brucei brucei while the rest served as uninfected controls. All the infected dogs developed trypanosomosis between Days 4 and 8 postinfection. The packed cell volume, haemoglobin concentration, total red blood cell count and white blood cell count decreased progressively indicating anaemia and leucopenia. The absolute reticulocyte counts were increased. Splenectomy enhanced fever, reticulocytosis and parasitaemia but delayed the onset of anaemia and leucopenia. It also shortened the prepatent period of the infection. The treatment of the infected dogs with diminazene aceturate (Berenil at the dose rate of 7.0 mg kg-1 body weight on Day 21 postinfection cleared the parasites in blood within 24 h and resulted in complete reversal of all the haematological aberrations observed. Splenectomy did not enhance or inhibit the recovery rate in the animals after treatment.