ABSTRACT
B-lymphocytes are migrating cells that specialize in antigen presentation, antibody secretion, and endocytosis; these processes implicate the modulation of plasma membrane elasticity. Cell stiffness is a force generated by the interaction between the actin-cytoskeleton and the plasma membrane, which requires the participation of several proteins. These proteins include class I myosins, which are now considered to play a role in controlling membrane-cytoskeleton interactions. In this study, we identified the motor protein Myosin 1g (Myo1g) as a mediator of this phenomenon. The absence of Myo1g decreased the cell stiffness, affecting cell adhesion, cell spreading, phagocytosis, and endocytosis in B-lymphocytes. The results described here reveal a novel molecular mechanism by which Myo1g mediates and regulates cell stiffness in B-lymphocytes. © 2016 Wiley Periodicals, Inc.
Subject(s)
Actin Cytoskeleton/metabolism , B-Lymphocytes/metabolism , Cell Membrane/metabolism , Endocytosis/physiology , Minor Histocompatibility Antigens/metabolism , Myosins/metabolism , Phagocytosis/physiology , Actin Cytoskeleton/genetics , Animals , B-Lymphocytes/cytology , Cell Adhesion/physiology , Cell Membrane/genetics , Female , Mice , Mice, Knockout , Minor Histocompatibility Antigens/genetics , Myosins/geneticsABSTRACT
El presente escrito tiene por objeto trasmitir el análisis y conclusión a las que se llegó respecto del estudio de un discurso de un alto prelado de la Arquidiócesis de Guadalajara. El análisis se hizo por medio del Algoritmo David Liberman, en su modalidad de actos del habla y la ponderación del contenido desde las claves de la argumentación. El artículo contiene cuatro apartados, en el primero se hace la presentación del tema, en el segundo, se expone el marco teórico referencial, en el tercero, se presentan los resultados y se evidencia el análisis del discurso. Finalmente, en el cuarto se discute y se puntualizan las conclusiones de este trabajo.
Subject(s)
Verbal Behavior , Speech , Psychology , ReligionABSTRACT
El presente escrito tiene por objeto trasmitir el análisis y conclusión a las que se llegó respecto del estudio de un discurso de un alto prelado de la Arquidiócesis de Guadalajara. El análisis se hizo por medio del Algoritmo David Liberman, en su modalidad de actos del habla y la ponderación del contenido desde las claves de la argumentación. El artículo contiene cuatro apartados, en el primero se hace la presentación del tema, en el segundo, se expone el marco teórico referencial, en el tercero, se presentan los resultados y se evidencia el análisis del discurso. Finalmente, en el cuarto se discute y se puntualizan las conclusiones de este trabajo. (AU)
Subject(s)
Psychology , Speech , Verbal Behavior , ReligionABSTRACT
El presente escrito tiene por objeto trasmitir el análisis y conclusión a las que se llegó respecto del estudio de un discurso de un alto prelado de la Arquidiócesis de Guadalajara. El análisis se hizo por medio del Algoritmo David Liberman, en su modalidad de actos del habla y la ponderación del contenido desde las claves de la argumentación. El artículo contiene cuatro apartados, en el primero se hace la presentación del tema, en el segundo, se expone el marco teórico referencial, en el tercero, se presentan los resultados y se evidencia el análisis del discurso. Finalmente, en el cuarto se discute y se puntualizan las conclusiones de este trabajo. (AU)
Subject(s)
Psychology , Speech , Verbal Behavior , ReligionABSTRACT
The modified Gouy-Chapman (MGC) theory has been used to study the electrical double layer near two charged plates immersed in a model electrolyte. The effects of assigning to the cations and anions different distances of closest approach to the charged surfaces are examined. The dependence of overcharging and charge reversal on the system parameters such as concentration, ion size and valence, is investigated both inside and outside the charged slit.
ABSTRACT
A system of globally coupled logistic maps with sequential updating is analyzed numerically. It is found that deterministic asynchronous updating schemes may have dramatical influences on the dynamical behaviors of globally coupled systems. Transitions from spatiotemporal chaos to spatially organized states are observed as the coupling parameter varies. It is shown that the model system may exhibit a variety of collective properties such as clustering, traveling wave patterns, and spatial bifurcation cascades.
ABSTRACT
The dynamical behavior of a Chern-Simons-Higgs system is studied for the spatially homogeneous case. The model, described by interacting gauge and scalar fields includes two parity and time violating terms: the Chern-Simons and the scalar magnetic interaction. For the pure Chern-Simons part of the theory the equations of motion are integrable. In general, however, the model system exhibits an interesting pattern of chaos to order transitions as the scalar magnetic moment is varied. The system dynamics are analyzed using a combination of standard techniques, such as phase space portraits, Lyapunov exponents, and Fourier spectra. (c) 2000 American Institute of Physics.
ABSTRACT
Spontaneous massive necrotizing herpesvirus bursitis, thymusitis, and splenitis in pale broiler chicks is described. Fibrinopurulent inflammation and necrosis was accompanied by intralesional solitary large dark blue intranuclear inclusion bodies in histiocytes and lymphocytes. Ultrastructurally, swollen nuclei with marginated chromatin contained herpesvirus.
Subject(s)
Bursitis/veterinary , Herpesviridae Infections/veterinary , Poultry Diseases/pathology , Poultry Diseases/virology , Splenic Diseases/veterinary , Thymus Gland , Animals , Bursitis/pathology , Bursitis/virology , Herpesviridae Infections/pathology , Herpesviridae Infections/physiopathology , Lymphatic Diseases/pathology , Lymphatic Diseases/virology , Necrosis/veterinary , Poultry Diseases/physiopathology , Splenic Diseases/pathology , Splenic Diseases/physiopathology , Splenic Diseases/virology , Thymus Gland/pathologyABSTRACT
It is known that in the transcription of genetic information there are ambiguities, i.e. the fact that a triplet codes for several aminoacids. This has generally been taken as due to errors in the transcription mechanisms. However, it has been postulated that instead of accidental miscoding, ambiguities are part of the expression of a generalized genetic code which depends on biological context. Group theory has been used to find the generalized genetic code. Here we present a new group theory approach which we think removes some weaknesses of previous works. The generalized genetic code presented here is different from that previously reported. We compare our results with experimental evidence and discuss the predictions presented.
Subject(s)
Genetic Code , Amino Acids , Codon , Transcription, GeneticSubject(s)
Calcium, Dietary/metabolism , Chickens/metabolism , Cholecalciferol/metabolism , Phosphorus/metabolism , Animal Feed , Animals , Body Weight , Bone Resorption , Calcium/blood , Calcium/deficiency , Egg Shell/ultrastructure , Female , Intestinal Absorption , Intestine, Small/metabolism , Oviposition , Paralysis/veterinary , Parathyroid Glands/pathology , Phosphorus/deficiency , Poultry Diseases/pathology , Protein Binding , Uterus/metabolism , Vitamin D Deficiency/veterinaryABSTRACT
Since 1975, the authors have been working in immunochemotherapy in acute myeloblastic leukemic adult patients. Autologous leukemic leucocytoblasts have been cultured with Vibrio cholerae neuraminidase (NVC). Thereafter, neoplastic cells were radiated with cobalt bomb and frozen to--70degreesC and afterwards 5 X 10(6) of these leucoblasts were intradermally injected to each patients every eight days. All patients received between six to ten injections. So far, ten such patients have been followed during eight months and since two months after immunization no leukemic leucocytoblasts in bone marrow and blood have been detected. Hemoglobin and platelets have increased more rapidly than usual and no patient has developed side effects. Phagocytosis tests carried out in vitro during remission, have demonstrated that only in immunized patients neutrophils and monocytes were capable of phagocytosis of autologous cultured malignant leucocytoblasts. The nucleus of phagocytized malignant cells was small and basophylic. These so far undescribed phagocytic cells were named post-immunization cells.
Subject(s)
Immunotherapy , Leukemia, Myeloid, Acute/therapy , Phagocytosis , Adolescent , Adult , Evaluation Studies as Topic , Female , Humans , Immunization , Male , Monocytes/immunology , Neutrophils/immunologyABSTRACT
The presence of virus particles and their possible mechanism of excretion was studied in cells of murine lymphoma L 5178Y grown intraperitoneally. Malignant cells obtained from 10 mice bearing this lymphoma were studied with the electron microscope. In 96% of the cells 1 to 30 viroid particles (VP) type C (VP-C) clumped or isolated, averaging six per cell were observed. Isolated VP-C were commonly seen within endoplasmic reticulum or budding off from cell surface. Such a finding was regarded as the mechanism of excretion. However, with the present findings it is not possible to draw any conclusions upon the oncogenic nature of the virus particles described herein.
Subject(s)
Inclusion Bodies, Viral/ultrastructure , Lymphoma/pathology , Oncogenic Viruses/ultrastructure , Peritoneal Neoplasms/pathology , AnimalsABSTRACT
Inclusion body hepatitis is recorded for the first time in Mexico. Clinical signs, gross lesions, and histopathology in chickens are described, as well as chicken embryo lesions.
