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1.
Trends Biochem Sci ; 47(2): 149-159, 2022 02.
Article in English | MEDLINE | ID: mdl-34887149

ABSTRACT

The difficulty of faithfully recapitulating malarial protein complexes in heterologous expression systems has long impeded structural study for much of the Plasmodium falciparum proteome. However, recent advances in single-particle cryo electron microscopy (cryoEM) now enable structure determination at atomic resolution with significantly reduced requirements for both sample quantity and purity. Combined with recent developments in gene editing, these advances open the door to structure determination and structural proteomics of macromolecular complexes enriched directly from P. falciparum parasites. Furthermore, the combination of cryoEM with the rapidly emerging use of in situ cryo electron tomography (cryoET) to directly visualize ultrastructures and protein complexes in the native cellular context will yield exciting new insights into the molecular machinery underpinning malaria parasite biology and pathogenesis.


Subject(s)
Malaria, Falciparum , Malaria , Parasites , Animals , Cryoelectron Microscopy/methods , Malaria, Falciparum/parasitology , Plasmodium falciparum/metabolism
2.
Nucleic Acids Res ; 49(6): 3461-3489, 2021 04 06.
Article in English | MEDLINE | ID: mdl-33398329

ABSTRACT

LARP1 is a key repressor of TOP mRNA translation. It binds the m7Gppp cap moiety and the adjacent 5'TOP motif of TOP mRNAs, thus impeding the assembly of the eIF4F complex on these transcripts. mTORC1 controls TOP mRNA translation via LARP1, but the details of the mechanism are unclear. Herein we elucidate the mechanism by which mTORC1 controls LARP1's translation repression activity. We demonstrate that mTORC1 phosphorylates LARP1 in vitro and in vivo, activities that are efficiently inhibited by rapamycin and torin1. We uncover 26 rapamycin-sensitive phospho-serine and -threonine residues on LARP1 that are distributed in 7 clusters. Our data show that phosphorylation of a cluster of residues located proximally to the m7Gppp cap-binding DM15 region is particularly sensitive to rapamycin and regulates both the RNA-binding and the translation inhibitory activities of LARP1. Our results unravel a new model of translation control in which the La module (LaMod) and DM15 region of LARP1, both of which can directly interact with TOP mRNA, are differentially regulated: the LaMod remains constitutively bound to PABP (irrespective of the activation status of mTORC1), while the C-terminal DM15 'pendular hook' engages the TOP mRNA 5'-end to repress translation, but only in conditions of mTORC1 inhibition.


Subject(s)
Autoantigens/metabolism , Mechanistic Target of Rapamycin Complex 1/metabolism , Protein Biosynthesis , Ribonucleoproteins/metabolism , Amino Acid Motifs , Autoantigens/chemistry , HEK293 Cells , Humans , Naphthyridines/pharmacology , Phosphorylation/drug effects , Protein Binding , Ribonucleoproteins/chemistry , Serine/metabolism , Sirolimus/pharmacology , Threonine/metabolism , Tyrosine/metabolism , SS-B Antigen
3.
Nat Commun ; 11(1): 5873, 2020 Nov 12.
Article in English | MEDLINE | ID: mdl-33184271

ABSTRACT

A Correction to this paper has been published: https://doi.org/10.1038/s41467-020-19845-z .

4.
Nat Commun ; 11(1): 5101, 2020 10 09.
Article in English | MEDLINE | ID: mdl-33037208

ABSTRACT

Urease converts urea into ammonia and carbon dioxide and makes urea available as a nitrogen source for all forms of life except animals. In human bacterial pathogens, ureases also aid in the invasion of acidic environments such as the stomach by raising the surrounding pH. Here, we report the structure of urease from the pathogen Yersinia enterocolitica at 2 Å resolution from cryo-electron microscopy. Y. enterocolitica urease is a dodecameric assembly of a trimer of three protein chains, ureA, ureB and ureC. The high data quality enables detailed visualization of the urease bimetal active site and of the impact of radiation damage. The obtained structure is of sufficient quality to support drug development efforts.


Subject(s)
Bacterial Proteins/chemistry , Bacterial Proteins/metabolism , Urease/chemistry , Urease/metabolism , Yersinia enterocolitica/enzymology , Catalytic Domain , Cryoelectron Microscopy , Lysine/metabolism , Models, Molecular , Nickel/chemistry , Nickel/metabolism , Protein Conformation , Protein Domains , Water/chemistry
5.
Nat Immunol ; 16(5): 476-484, 2015 May.
Article in English | MEDLINE | ID: mdl-25774716

ABSTRACT

The AIM2 inflammasome detects double-stranded DNA in the cytosol and induces caspase-1-dependent pyroptosis as well as release of the inflammatory cytokines interleukin 1ß (IL-1ß) and IL-18. AIM2 is critical for host defense against DNA viruses and bacteria that replicate in the cytosol, such as Francisella tularensis subspecies novicida (F. novicida). The activation of AIM2 by F. novicida requires bacteriolysis, yet whether this process is accidental or is a host-driven immunological mechanism has remained unclear. By screening nearly 500 interferon-stimulated genes (ISGs) through the use of small interfering RNA (siRNA), we identified guanylate-binding proteins GBP2 and GBP5 as key activators of AIM2 during infection with F. novicida. We confirmed their prominent role in vitro and in a mouse model of tularemia. Mechanistically, these two GBPs targeted cytosolic F. novicida and promoted bacteriolysis. Thus, in addition to their role in host defense against vacuolar pathogens, GBPs also facilitate the presentation of ligands by directly attacking cytosolic bacteria.


Subject(s)
Bacteriolysis , DNA-Binding Proteins/metabolism , Francisella tularensis/physiology , GTP-Binding Proteins/metabolism , Inflammasomes/metabolism , Tularemia/immunology , Animals , Cells, Cultured , Cytosol/microbiology , DNA-Binding Proteins/genetics , Disease Models, Animal , GTP-Binding Proteins/genetics , Humans , Mice , Mice, Knockout , RNA, Small Interfering/genetics
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