[Diagnostic accuracy of blood pressure determination in clinics in control of hypertension: Proposal of new cut-off values]. / Precisión diagnóstica de la determinación de la presión arterial en consulta en el control del hipertenso: Propuesta de nuevos valores de corte.

AIM: This study seeks to determine the optimal cut-off values for the determination of the blood pressure in the clinic as a follow-up test in Primary Care practice. DESIGN: A total of 153 hypertensive patients under 80years of age who met inclusion and exclusion criteria for the study, were subjected to ambulatory monitoring of their blood pressure for 24hours (ABPM). After which two clinic-based measurements were obtained. With the results obtained from the clinic, and taking the ABMP as a reference, the ROC curve was calculated choose the optimal cut-off point. The agreement between both measurements was determined by the intraclass correlation coefficient and the Bland-Altman equation. A validation study was then carried out with the objective of diagnosing whether or not the hypertensive patient was in control. RESULTS: The optimal cut-off values were 137mmHg for systolic BP (sensitivity: 89.3%; specificity: 72.2%) and 84mmHg for diastolic blood pressure (sensitivity: 79.4%; specificity: 72.3%). The agreement in the diagnosis of control between clinic-based measurement and ABPM was 58.9% (Kappa: 0.418). CONCLUSION: The optimal cut-off value of the diastolic BP for follow-up is lower than the values currently established.

[Local variability in the guidelines for hypertension screening, diagnosis and baseline assessment in 40 health centers]. / Variabilidad en la protocolización de la captación, diagnóstico y valoración inicial del paciente hipertenso en 40 centros de salud.

BACKGROUND: To identify and to compare the recommendations of the currently existing clinical protocols for arterial hypertension (HT) screening, diagnosis and baseline study in the Murcia Region, Spain, in order to assess their validity and reliability. MATERIAL AND METHODS: Structured comparative description of guidelines in Clinical Protocols for HT (n = 40) from all Health Centers in the Region. Comparative description of the Health Centers Clinical Protocols and national and international reference documents regarding HT screening, diagnosis and baseline assessment is performed. RESULTS: Target population for screening ranged from aged > or = 14 years (24 centers) to > 20 (1 center). Screening procedure was not described in 3 centers and screening periodicity varied from 6 months to 5 years, with differences by age and by concurrent risk factors in some protocols. Diagnosis norms differed by age in only 11 protocols; blood pressure (BP) exams for diagnosis varied from 3 to 5 and there were 12 different HT figures for immediate diagnosis and treatment. Baseline study varied greatly; we found a total of 7 recommended items for anamnesis, 11 for physical exam, 19 for blood tests, 4 for urine tests, and 7 for other tests; only the electrocardiogram was common to all protocols. There was also some disagreement in the national and international reference guidelines whose scientific evidence was not always explicitly considered. CONCLUSIONS: There is a wide local variation in norms for HT screening, diagnosis, and baseline study. If the Centers follow their own protocols, the same patient may be or may not be diagnosed depending on the Center he visits; additionally, there would be big differences in the process and resources needed for HT control. This variation casts doubt on the protocols validity and reliability at regional level and underscores the need for critically reviewing the validity of existing protocols.

[Diagnosis of mild arterial hypertension: 3 or 5 measurements?]. / Diagnóstico de hipertensión arterial ligera: tres o cinco tomas?

OBJECTIVE: To determine the differences in the diagnosis of light arterial hypertension between using three tension measurements (over 2 or 3 weeks) or five (6 or 7 weeks). DESIGN: A descriptive, longitudinal study. SETTING: Santomera Health Centre, Murcia. PARTICIPANTS: In 245 patients initially diagnosed as suffering from light Hypertension (with three takes of blood pressure), two more tension measurements were taken, over four weeks, to make a definitive diagnosis of light arterial Hypertension. 156 patients completed the series, for whom the differences in diagnosis between three and five takes, and variations in their blood pressure figures on repetition of the measurements, were studied. There were no differences in patients' basic characteristics between those who completed the tests and those who didn't. But there were differences between different professionals regarding their compliance with the procedure. RESULTS: 19.9% of the patients diagnosed initially after three blood pressure measurements did not have the diagnosis confirmed after five. Repetition of the measurements signified decrease in diastolic (p < 0.00005) and systolic (p < 0.00005) pressures. Average age of patients definitely diagnosed as having light hypertension was higher than the age of those not confirmed (p < 0.005). The older the patient, the higher the systolic pressure (p < 0.0001). CONCLUSIONS: The diagnosis of light arterial hypertension differed in 19.9% between three measurements and five.

SwaI, a unique restriction endonuclease from Staphylococcus warneri, which recognizes 5'-ATTTAAAT-3'.

A novel class-II restriction endonuclease designated SwaI was purified from Staphylococcus warneri. This enzyme cleaves adenovirus 2 DNA, SV40 DNA and M13mp7 at one site each, but does not cleave lambda, PhiX174, pBR322 or pBR328 DNA. SwaI recognizes the octanucleotide sequence 5'-ATTTAAAT-3', cleaving in the center of the recognition sequence creating blunt ended DNA fragments. SwaI was used to digest chromosomal DNA from various microorganisms and human cells.