Plant triterpenoid saponins function as susceptibility factors to promote the pathogenicity of Botrytis cinerea.

The gray mold fungus Botrytis cinerea is a necrotrophic pathogen that causes diseases in hundreds of plant species, including high-value crops. Its polyxenous nature and pathogenic success are due to its ability to perceive host signals in its favor. In this study, we found that laticifer cells of Euphorbia lathyris are a source of susceptibility factors required by B. cinerea to cause disease. Consequently, poor-in-latex (pil) mutants, which lack laticifer cells, show full resistance to this pathogen, whereas lot-of-latex mutants, which produce more laticifer cells, are hypersusceptible. These S factors are triterpenoid saponins, which are widely distributed natural products of vast structural diversity. The downregulation of laticifer-specific oxydosqualene cyclase genes, which encode the first committed step enzymes for triterpene and, therefore, saponin biosynthesis, conferred disease resistance to B. cinerea. Likewise, the Medicago truncatula lha-1 mutant, compromised in triterpenoid saponin biosynthesis, showed enhanced resistance. Interestingly, the application of different purified triterpenoid saponins pharmacologically complemented the disease-resistant phenotype of pil and hla-1 mutants and enhanced disease susceptibility in different plant species. We found that triterpenoid saponins function as plant cues that signal transcriptional reprogramming in B. cinerea, leading to a change in its growth habit and infection strategy, culminating in the abundant formation of infection cushions, the multicellular appressoria apparatus dedicated to plant penetration and biomass destruction in B. cinerea. Taken together, these results provide an explanation for how plant triterpenoid saponins function as disease susceptibility factors to promote B. cinerea pathogenicity.

Practical high-dimensional quantum key distribution protocol over deployed multicore fiber.

Quantum key distribution (QKD) is a secure communication scheme for sharing symmetric cryptographic keys based on the laws of quantum physics, and is considered a key player in the realm of cyber-security. A critical challenge for QKD systems comes from the fact that the ever-increasing rates at which digital data are transmitted require more and more performing sources of quantum keys, primarily in terms of secret key generation rate. High-dimensional QKD based on path encoding has been proposed as a candidate approach to address this challenge. However, while proof-of-principle demonstrations based on lab experiments have been reported in the literature, demonstrations in realistic environments are still missing. Here we report the generation of secret keys in a 4-dimensional hybrid time-path-encoded QKD system over a 52-km deployed multicore fiber link forming by looping back two cores of a 26-km 4-core optical fiber. Our results indicate that robust high-dimensional QKD can be implemented in a realistic environment by combining standard telecom equipment with emerging multicore fiber technology.

Dependence of nonlinear interference on mode dispersion and modulation format in strongly-coupled SDM transmissions.

We study the role of the modulation format in the interaction between mode dispersion and the fiber nonlinear interference (NLI) in space-division multiplexed (SDM) systems with strongly-coupled spatial modes. We show that the interplay between mode dispersion and the modulation format has a significant impact on the magnitude of cross-phase modulation (XPM). We propose a simple formula that accounts for the modulation-format dependence of the XPM variance in the presence of arbitrary levels of mode dispersion, thus extending the ergodic Gaussian noise model.

Enhancing the Kramers-Kronig receiver via dispersion-based spatial diversity.

We report a scheme for reconstructing the complex envelope of an optical signal from two decorrelated measurements of its intensity. The decorrelation is achieved by splitting the received optical signal into two copies, and by dispersing one of the copies prior to photo detection. The reconstructed complex-valued signal is obtained by means of an iterative algorithm that requires only a few tens of iterations. The starting point of the search procedure is produced by Kramers-Kronig (KK) reconstruction. With this procedure, the continuous-wave tone that accompanies the received signal is reduced by 5 dB to 6 dB compared to the requirement of a KK receiver alone.

Role of polarization-mode coupling in the crosstalk between cores of weakly-coupled multi-core fibers.

We develop a theory of crosstalk in weakly-coupled multi-core fibers that accounts for the effect of intra-core polarization-mode coupling. We show that random polarization-mode coupling plays a critical role, just like other polarization-independent effects such as fiber bending and twist, in explaining the observed incoherent crosstalk in weakly-coupled multi-core fibers.

Simple method for optimizing the DC bias of Kramers-Kronig receivers based on AC-coupled photodetectors.

We propose and evaluate a method to estimate the DC bias required for AC-coupled Kramers-Kronig receivers. The proposed method is based on a spectral analysis of the reconstructed signal without requiring an evaluation of the signal quality. The proposed method is described analytically and demonstrated experimentally using 12.5 GBaud 16-ary quadrature-amplitude modulated signals in back-to-back and after 100 km transmission.

Characterization of the Copper Transporters from Lotus spp. and Their Involvement under Flooding Conditions.

Forage legumes are an important livestock nutritional resource, which includes essential metals, such as copper. Particularly, the high prevalence of hypocuprosis causes important economic losses to Argentinian cattle agrosystems. Copper deficiency in cattle is partially due to its low content in forage produced by natural grassland, and is exacerbated by flooding conditions. Previous results indicated that incorporation of Lotus spp. into natural grassland increases forage nutritional quality, including higher copper levels. However, the biological processes and molecular mechanisms involved in copper uptake by Lotus spp. remain poorly understood. Here, we identify four genes that encode putative members of the Lotus copper transporter family, denoted COPT in higher plants. A heterologous functional complementation assay of the Saccharomyces cerevisiae ctr1∆ctr3∆ strain, which lacks the corresponding yeast copper transporters, with the putative Lotus COPT proteins shows a partial rescue of the yeast phenotypes in restrictive media. Under partial submergence conditions, the copper content of L. japonicus plants decreases and the expression of two Lotus COPT genes is induced. These results strongly suggest that the Lotus COPT proteins identified in this work function in copper uptake. In addition, the fact that environmental conditions affect the expression of certain COPT genes supports their involvement in adaptive mechanisms and envisages putative biotechnological strategies to improve cattle copper nutrition.

Interspecific hybridization improves the performance of Lotus spp. under saline stress.

Salinity is one of the most frequent limiting conditions in pasture production for grazing livestock. Legumes, such as Lotus spp. with high forage quality and capable of adapting to different environments, improves pasture performance in restrictive areas. In order to determine potential cultivars with better forage traits, the current study assess the response to salt stress of L. tenuis, L. corniculatus and a novel L. tenuis x L. corniculatus accession. For this purpose, chlorophyll fluorescence, biomass production, ion accumulation and anthocyanins and proanthocyanidins levels have been evaluated in control and salt-treated plants PSII activity was affected by salt in L. tenuis, but not in L. corniculatus or hybrid plants. Analyzed accessions showed similar values of biomass, Na+ and K+ levels after salt treatment. Increasing Cl- concentrations were observed in all accessions. However, hybrid plants accumulate Cl- in stems at higher levels than their parental. At the same time, the levels of anthocyanins considerably increased in L. tenuis x L. corniculatus stems. Chloride and anthocyanin accumulation in stems could explain the best performance of hybrid plants after a long saline treatment. Finally, as proanthocyanidins levels were no affected by salt, L. tenuis x L. corniculatus plants maintained adequate levels to be used as ruminant feed. In conclusion, these results suggest that hybrid plants have a high potential to be used as forage on salt-affected lands. High Cl- and anthocyanins accumulation in Lotus spp. stems seems to be a trait associated to salinity tolerance, with the possibility of being used in legume breeding programs.

Nonlinear interference noise in space-division multiplexed transmission through optical fibers.

We study the nonlinear interference noise (NLIN) generated in SDM systems, and generalize the NLIN model introduced in the context of single-mode fibers to the multi-mode case. The generalized model accounts for the modulation-format dependence of the NLIN, and gives the scaling of the NLIN power with the number of transmitted modes. It also provides the tools for extending the results of the NLIN Wizard to SDM. Unlike in the case of single-mode systems, the effect of MD cannot in general be ignored in the SDM case. We show that inclusion of MD erases the contribution of FWM effects, and significantly suppresses the effect of XPM.

The R2R3-MYB TT2b and the bHLH TT8 genes are the major regulators of proanthocyanidin biosynthesis in the leaves of Lotus species.

MAIN CONCLUSION: By exploiting interspecific hybrids and their progeny, we identified key regulatory and transporter genes intimately related to proanthocyanidin biosynthesis in leaves of Lotus spp. Proanthocyanidins (PAs), known as condensed tannins, are polymeric flavonoids enriching forage legumes of key nutritional value to prevent bloating in ruminant animals. Unfortunately, major forage legumes such as alfalfa and clovers lack PAs in edible tissues. Therefore, engineering the PA trait in herbage of forage legumes is paramount to improve both ecological and economical sustainability of cattle production system. Progresses on the understanding of genetic determinants controlling PA biosynthesis and accumulation have been mainly made studying mutants of Arabidopsis, Medicago truncatula and Lotus japonicus, model species unable to synthesize PAs in the leaves. Here, we exploited interspecific hybrids between Lotus corniculatus, with high levels of PAs in the leaves, and Lotus tenuis, with no PAs in these organs, and relative F2 progeny, to identify among candidate PA regulators and transporters the genes mainly affecting this trait. We found that the levels of leaf PAs significantly correlate with the expression of MATE1, the putative transporter of glycosylated PA monomers, and, among the candidate regulatory genes, with the expression of the MYB genes TT2a, TT2b and MYB14 and the bHLH gene TT8. The expression levels of TT2b and TT8 also correlated with those of all key structural genes of the PA pathways investigated, MATE1 included. Our study unveils a different involvement of the three Lotus TT2 paralogs to the PA trait and highlights differences in the regulation of this trait in our Lotus genotypes with respect to model species. This information opens new avenues for breeding bloat safe forage legumes.

The alkaline tolerance in Lotus japonicus is associated with mechanisms of iron acquisition and modification of the architectural pattern of the root.

The response of fifty-four Lotus japonicus ecotypes, and of six selected ecotypes was investigated under alkaline conditions. Sensitive, but not tolerant ecotypes, showed interveinal chlorosis under all alkalinity conditions and high mortality under extreme alkalinity. Interveinal chlorosis was associated with Fe deficiency, as a reduced Fe2+ shoot content was observed in all sensitive ecotypes. In addition, some showed a decline in photosynthesis rate and PSII performance compared to the control. In contrast, some tolerant ecotypes did not change these parameters between treatments. Alkaline tolerance could be explained by a mechanism of Fe acquisition and a root structural modification. This conclusion was based on the fact that all tolerant, but not the sensitive ecotypes, presented high ferric reductase oxidase activity under alkaline stress compared to the control, and a Herringbone root pattern modification. On this basis, the analysis of these mechanisms of alkaline tolerance could be used in screening programs for the selection of new tolerant genotypes in the Lotus genus.

The delay spread in fibers for SDM transmission: dependence on fiber parameters and perturbations.

Contrary to single mode fibers, where random imperfections are responsible for polarization-mode dispersion, modal dispersion (MD) in multi-mode fiber structures for space-division multiplexed (SDM) transmission, originates chiefly from the intrinsic non-degeneracy of the propagating modes, also known as modal birefringence. The presence of random imperfections in such fibers has a positive aspect, as it reduces the intrinsic MD, and in the limit of strong coupling it causes the signal delay spread to increase with the square root of the propagation distance, rather than linearly, as would be the case in an ideal fiber. In this paper we derive a formula that relates the signal delay spread to the fiber geometry and to the statistical properties of the structural fiber perturbations. The derived formula provides insight into the MD phenomenon and facilitates the design of low-MD multi-mode fiber structures.

Modeling and performance metrics of MIMO-SDM systems with different amplification schemes in the presence of mode-dependent loss.

Mode-dependent loss (MDL) is a major factor limiting the achievable information rate in multiple-input multiple-output space-division multiplexed systems. In this paper we show that its impact on system performance, which we quantify in terms of the capacity reduction relative to a reference MDL-free system, may depend strongly on the operation of the inline optical amplifiers. This dependency is particularly strong in low mode-count systems. In addition, we discuss ways in which the signal-to-noise ratio of the MDL-free reference system can be defined and quantify the differences in the predicted capacity loss. Finally, we stress the importance of correctly accounting for the effect of MDL on the accumulation of amplification noise.

Intensity impulse response of SDM links.

We study the response of space-division multiplexed fiber links to an excitation by a short impulse of the optical intensity. We show that, in the presence of full mixing, the intensity impulse response is Gaussian, confirming recently reported experimental observations, and relate its variance to the mean square of the mode dispersion vector of the link τ(->). The good agreement between our theory and the previously published experiments provides solid foundations to the random coupling model of SDM fiber links, and provides a tool for efficient design of MIMO-DSP receivers.

Response to long-term NaHCO3-derived alkalinity in model Lotus japonicus Ecotypes Gifu B-129 and Miyakojima MG-20: transcriptomic profiling and physiological characterization.

The current knowledge regarding transcriptomic changes induced by alkalinity on plants is scarce and limited to studies where plants were subjected to the alkaline salt for periods not longer than 48 h, so there is no information available regarding the regulation of genes involved in the generation of a new homeostatic cellular condition after long-term alkaline stress. Lotus japonicus is a model legume broadly used to study many important physiological processes including biotic interactions and biotic and abiotic stresses. In the present study, we characterized phenotipically the response to alkaline stress of the most widely used L. japonicus ecotypes, Gifu B-129 and MG-20, and analyzed global transcriptome of plants subjected to 10 mM NaHCO3 during 21 days, by using the Affymetrix Lotus japonicus GeneChip®. Plant growth assessment, gas exchange parameters, chlorophyll a fluorescence transient (OJIP) analysis and metal accumulation supported the notion that MG-20 plants displayed a higher tolerance level to alkaline stress than Gifu B-129. Overall, 407 and 459 probe sets were regulated in MG-20 and Gifu B-129, respectively. The number of probe sets differentially expressed in roots was higher than that of shoots, regardless the ecotype. Gifu B-129 and MG-20 also differed in their regulation of genes that could play important roles in the generation of a new Fe/Zn homeostatic cellular condition, synthesis of plant compounds involved in stress response, protein-degradation, damage repair and root senescence, as well as in glycolysis, gluconeogenesis and TCA. In addition, there were differences between both ecotypes in the expression patterns of putative transcription factors that could determine distinct arrangements of flavonoid and isoflavonoid compounds. Our results provided a set of selected, differentially expressed genes deserving further investigation and suggested that the L. japonicus ecotypes could constitute a useful model to search for common and distinct tolerance mechanisms to long-term alkaline stress response in plants.

Random coupling between groups of degenerate fiber modes in mode multiplexed transmission.

We study random coupling induced crosstalk between groups of degenerate modes in spatially multiplexed optical transmission. Our analysis shows that the average crosstalk is primarily determined by the wavenumber mismatch, by the correlation length of the random perturbations, and by the coherence length of the degenerate modes, whereas the effect of a deterministic group velocity difference is negligible. The standard deviation of the crosstalk is shown to be comparable to its average value, implying that crosstalk measurements are inherently noisy.

Raman amplification in multimode fibers with random mode coupling.

We present the theory of Raman amplification in long multimode optical fibers, where strong random mode coupling within groups of quasi-degenerate modes is unavoidable. In such fibers, the signal components in modes that belong to the same strongly coupled group experience the same Raman amplification, where the differential gain is linearly dependent on the aggregate powers of the pump in each of the mode groups. The equations that we derive significantly facilitate the numerical and analytical study of Raman amplification in long multimode fibers.

Coupled Manakov equations in multimode fibers with strongly coupled groups of modes.

We derive the fundamental equations describing nonlinear propagation in multi-mode fibers in the presence of random mode coupling within quasi-degenerate groups of modes. Our result generalizes the Manakov equation describing mode coupling between polarizations in single-mode fibers. Nonlinear compensation of the modal dispersion is predicted and tested via computer simulations.

Nonlinear propagation in multi-mode fibers in the strong coupling regime.

We show that light propagation in a group of degenerate modes of a multi-mode optical fiber in the presence of random mode coupling is described by a multi-component Manakov equation, thereby making multi-mode fibers the first reported physical system that admits true multi-component soliton solutions. The nonlinearity coefficient appearing in the equation is expressed rigorously in terms of the multi-mode fiber parameters.

Stokes-space analysis of modal dispersion in fibers with multiple mode transmission.

Modal dispersion (MD) in a multimode fiber may be considered as a generalized form of polarization mode dispersion (PMD) in single mode fibers. Using this analogy, we extend the formalism developed for PMD to characterize MD in fibers with multiple spatial modes. We introduce a MD vector defined in a D-dimensional extended Stokes space whose square length is the sum of the square group delays of the generalized principal states. For strong mode coupling, the MD vector undertakes a D-dimensional isotropic random walk, so that the distribution of its length is a chi distribution with D degrees of freedom. We also characterize the largest differential group delay, that is the difference between the delays of the fastest and the slowest principal states, and show that it too is very well approximated by a chi distribution, although in general with a smaller number of degrees of freedom. Finally, we study the spectral properties of MD in terms of the frequency autocorrelation functions of the MD vector, of the square modulus of the MD vector, and of the largest differential group delay. The analytical results are supported by extensive numerical simulations.