[Bactericidal activity of human, swine and cattle serum against pseudomonas aeruginosa strains]. / Bakteriobójcza aktywnosc surowicy ludzkiej, swinskiej i bydlecej wobec szczepów Pseudomonas aeruginosa.

The aim of this study was to assess of bactericidal activity of human, swine and cattle serum against 136 of Pseudomonas aeruginosa strains isolated from people, fishes, domestic and fur animals. The mechanism of the bactericidal activity of serum against gram-negative bacteria is complex and involves the participation of complement, antibodies and lysozyme (1, 5, 7, 8, 10, 11, 13, 14, 24, 25, 27, 30). The susceptibility of gram-negative rods to serum is differentiated. Pseudomonas aeruginosa strains are the most resistant (17, 25, 30). This opportunistic pathogen produce proteases that destroy complement components and immunoglobulins (3, 18, 19). The bactericidal activity of serum was determined after 3 hours incubation of bacteria in 50% serum by the method of Jankowski (1981) (5). The results of this study indicate that 71% of this strains were resistant to swine serum action, 68% of this strains were resistant to bovine serum and 57% of the Pseudomonas aeruginosa strains were sensitive to human serum. The P. aeruginosa strains isolated from fishes were the most sensitive to serum action and the strains isolated from people and cattle were most resistant to the bactericidal activity of serum.

Pathomorphological studies in European bison (Bison bonasus Linnaeus, 1758) with seropositive reaction to Coxiella burnetii.

Comprehensive serological and histopathological examinations of 47 free living European bison (Bison bonasus Linnaeus, 1758) were performed. Of these animals, 36 were serologically positive due to Coxiella burnetii, which confirmed the presence of Q fever epizootic foci in this population of wild animals in Poland. The presence of multiple foci of mononuclear cells typical for Q fever was a consistent finding in all tissues of the majority of C. burnetii seropositive animals under study. Pathomorphological changes observed in myocardium as the focal coagulation necrosis, and in kidneys, resembled the glomerular lesion observed in humans with Q fever, as well as in the experimental Q-fever infections in laboratory animals. These changes were absent in bison showing a C. burnetii seronegative reaction.

[Is Aujeszky's disease a zoonosis?]. / Czy choroba Aujeszkyego jest zoonoza?

Several cases of human illness with symptoms suggestive of Aujeszky's Disease (AD) at an outbreak of the disease in cattle and swine were evaluated during this study. Additionally serological studies of people in the group at high risk for illness caused by Aujeszky's Disease Virus (ADV) were carried out. In a herd of 180 bulls sudden clinical disease occurred with symptoms of muscular tremors, salivation, sweating and severe pruritus of the head. The clinical disease lasted two to eight hours and finished in death. In 7 days 10 bulls died and 60 were either killed or culled. A biological test for AD was positive. ADV was identified in the brain of a dead bull. Serological studies for the bulls were negative, however 92.9% on the premises farm were asymptomatically infected. On third to the fifth day of disease in the cattle clinical signs appeared in six of seven workers which had direct contact with diseased cattle. A pruritus of the palms, which spread onto the lower and upper arms, shoulders and back lasted several days.