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1.
Diagn Microbiol Infect Dis ; 95(3): 114854, 2019 Nov.
Article in English | MEDLINE | ID: mdl-31366440

ABSTRACT

We analyzed sequences of graSR, vraSR, walKR and rpoB genes in hVISA from Brazil. Five isolates showed mutations in at least one gene. rpoB H481N and graS T224I were the most frequent mutations, followed by graR D148Q and walK A468T. Our study reinforces the heterogeneity of genetic patterns among hVISA.


Subject(s)
Staphylococcus aureus/genetics , Vancomycin Resistance/genetics , Anti-Bacterial Agents/pharmacology , Bacterial Proteins/genetics , Humans , Microbial Sensitivity Tests , Mutation , Staphylococcal Infections/microbiology , Staphylococcus aureus/drug effects , Staphylococcus aureus/isolation & purification , Vancomycin/pharmacology
2.
J Forensic Odontostomatol ; 20(1): 6-9, 2002 Jun.
Article in English | MEDLINE | ID: mdl-12085524

ABSTRACT

Human identification can be accomplished by several technical procedures, especially by the comparative analysis of dental documents. Recent advances in molecular biology have now widened the scope for human identification and defined the protocols for the collection of biological material. Allele patterns of a population are particularly useful and they should be verified since they vary in different populations. We have studied the frequency of the alleles in three STR loci of the Y chromosome (DYS390, DYS391 and DYS393) in a group of Brazilian caucasian subjects. Results presented alleles 21, 22, 23, 24, 25 and 26 in locus DYS390, alleles 8, 9, 10, 11 12 and 13 in STR DYS391 and alleles in STR DYS393 were 11, 12, 13 14 and 15. The highest frequencies were 24 (0.46), 11 (0.37) and 13 (0.45). Each of the three STR systems had a PE (power of exclusion) of 0.6764 (DYS390), 0.5988 (DYS391) and 0.6136 (DYS393), The combination of the three STR systems revealed a PE of 0.9498, suggesting that this data can help in human identification.


Subject(s)
Forensic Anthropology/methods , Y Chromosome , Alleles , Brazil , Chromosome Mapping , Databases, Factual , Gene Frequency , Humans , Male , Minisatellite Repeats , Polymerase Chain Reaction
3.
Pesqui Odontol Bras ; 15(4): 314-9, 2001.
Article in Portuguese | MEDLINE | ID: mdl-11787320

ABSTRACT

There are several protocols reported in the literature for the extraction of genomic DNA from formalin-fixed paraffin-embedded samples. Genomic DNA is utilized in molecular analyses, including PCR. This study compares three different methods for the extraction of genomic DNA from formalin-fixed paraffin-embedded (inflammatory fibrous hyperplasia) and non-formalin-fixed (normal oral mucosa) samples: phenol with enzymatic digestion, and silica with and without enzymatic digestion. The amplification of DNA by means of the PCR technique was carried out with primers for the exon 7 of human keratin type 14. Amplicons were analyzed by means of electrophoresis in an 8% polyacrylamide gel with 5% glycerol, followed by silver-staining visualization. The phenol/enzymatic digestion and the silica/enzymatic digestion methods provided amplicons from both tissue samples. The method described is a potential aid in the establishment of the histopathologic diagnosis and in retrospective studies with archival paraffin-embedded samples.


Subject(s)
DNA/isolation & purification , Polymerase Chain Reaction/methods , Humans , Nucleic Acid Amplification Techniques , Paraffin Embedding
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