Psychometry and Cut-off Points of the Jefferson Scale of Empathy in Medical Students in Central America and the Caribbean.

OBJECTIVE: The objective of this study was to determine cut-off points that can be used to differentiate measures of empathy, which would then be classified as high, medium, or low. To do so, we used data from students from 7 medical schools in Colombia, El Salvador, and the Dominican Republic, after determining the psychometric properties of the 3-dimensional model of empathy in the Jefferson Scale of Empathy, S-version (for medical students). MATERIALS AND METHODS: This non-experimental descriptive study had a sample that consisted of 6291 students. The structure and factor invariance were analyzed by country and sex. A hierarchical cluster analysis and a bifactorial analysis of variance were applied. RESULTS: The measure of empathy was reliable on the global scale (α = .82; ω = .88). A confirmatory factor analysis showed that the original model was replicable and adjusted to the data (comparative fit index [CFI] = .90; goodness of fit index = .94), while the multigroup analysis allowed to assume an invariant factor structure by country and gender (ΔCFI < .01). Tables were constructed with cut off points for empathy and its dimensions. DISCUSSION AND CONCLUSION: Our study solves the problem of comparing the scores and the levels of empathy observed in the medical students at different schools of medicine, making said comparisons within and between countries and between genders. The instrument used has adequate psychometric properties and the cut-off values obtained allow the classifying of people with lower or higher levels of empathy.

In silico analysis of cross reactivity among phospholipases from Hymenoptera species.

Background: Phospholipases are enzymes with the capacity to hydrolyze membrane lipids and have been characterized in several allergenic sources, such as hymenoptera species. However, cross-reactivity among phospholipases allergens are little understood. The objective of this study was to determine potential antigenic regions involved in cross-reactivity among allergens of phospholipases using an in silico approach. Methods: In total, 18 amino acids sequences belonging to phospholipase family derived from species of the order hymenoptera were retrieved from the UniProt database to perform phylogenetic analysis to determine the closest molecular relationship. Multialignment was done to identify conserved regions and matched with antigenic regions predicted by ElliPro server. 3D models were obtained from modeling by homology and were used to locate cross-reactive antigenic regions. Results: Phylogenetic analysis showed that the 18 phospholipases split into four monophyletic clades (named here as A, B, C and D). Phospholipases from A clade shared an amino acid sequences' identity of 79%. Antigenic patches predicted by Ellipro were located in highly conserved regions, suggesting that they could be involved in cross-reactivity in this group (Ves v 1, Ves a 1 and Ves m 1). Conclusions: At this point, we advanced to the characterization of potential antigenic sites involved in cross-reactivity among phospholipases. Inhibition assays are needed to confirm our finding.

Levels of empathy, empathy decline and differences between genders in medical students of Cartagena (Colombia) / Niveles de empatía, declinación empática y diferencias entre géneros en estudiantes de medicina en Cartagena, Colombia

INTRODUCTION: Empathy helps to develop an appropriate doctor-patient relationship, which, according to the literature is susceptible to decline. Therefore, the purpose of the present study was to estimate the levels of empathy in general, and the potential for the growth of empathy in medical students. MATERIALS AND METHODS: This exploratory and cross-sectional study included students from the first to fifth academic year of the University Corporation Rafael Nunez (Cartagena, Colombia) (N = 971, n = 756, 77.86% of the population studied). Participants were given the Jefferson Scale of Empathy, Spanish version for medical students, validated and adapted in Colombia. A bifactorial variance analysis (model III) was used to find the mean differences between courses and genders, and the interaction between these two factors. Data was described using simple arithmetic graphs and processed with SPSS 20.0 to estimate the total potential for growth. RESULTS: Differences were found between academic years and gender in empathy in general and in the component of "compassionate care". CONCLUSION: The levels of empathy are relatively low, and the behavior of empathy levels does not match the concept of empathy decline. There are no gender differences and there is a considerable potential for growth of empathy, as well as its components

INTRODUCCIÓN: La empatía permite la adecuada relación médico-paciente y se plantea que es susceptible de disminuir según lo informado en la literatura. El propósito del presente estudio fue estimar los niveles de empatía en general y el potencial para el crecimiento empático en estudiantes de medicina. MATERIALES Y MÉTODOS: Este estudio exploratorio y transversal, incluyó estudiantes desde el primero al quinto año académico de la Corporación Universitaria Rafael Núñez, Cartagena, Colombia (n = 971, n = 756, 77,86% de la población estudiada). Los participantes recibieron la escala de empatía de Jefferson, versión en español para estudiantes de medicina, validada y adaptada en Chile. Se aplicó un análisis de varianza bifactorial (modelo III) para encontrar diferencias de medias entre cursos, géneros y la interacción entre estos 2 factores. Los datos se describieron usando gráficos aritméticos simples y se procesaron con SPSS® Statistics V.20.0 para estimar el potencial total de crecimiento. RESULTADOS: Se encontraron diferencias entre los años académicos y el género en la empatía en general, y en el componente de «atención compasiva». CONCLUSIÓN: Los niveles de empatía son relativamente bajos, y el comportamiento de los niveles de empatía no coincide con el concepto de disminución empática. No hay diferencias de género, y la estimación del potencial de crecimiento de la empatía y la de sus componentes plantea la necesidad de un urgente cambio curricular

Allergy to Mus m 1: Allergy to Mus m 1: A review of structural, and immunological features.

The prevalence of allergies to pets has been increasing over the past decades. Some of the most important animal-derived allergens are members of the lipocalin protein family, which are found in dander, saliva, and urine. These allergens disperse effectively and are widely present in indoor environments. Exposure to high levels of mouse urinary protein (Mus m 1, hereinafter called 'mouse allergen') has been previously linked to sensitization to mouse, and indicators of asthma severity or control in some studies. To date, this is the only known mouse allergen registered in the IUIS database. This allergen is responsible for 27% of the total T cell response, confirming the dominant role it plays in mouse allergy. Mice have a worldwide distribution affecting both rural and urban areas; hence humans are frequently exposed to mouse-derived proteins. Additionally, exposure to mouse allergens has increased since they are more frequently being made pets, and in addition, exposure of laboratory animal care personnel to mice has been associated with a high risk of developing occupational allergies. Mus m 1 has been recognized as the main mouse allergen, and several studies suggest its clinical relevance. What makes Mus m 1 such an important allergen? In this review, we explored its structural, immunological, and clinical features.

Study of interaction energies between residues of the active site of Hsp90 and geldanamycin analogues using quantum mechanics/molecular mechanics methods.

Background: Heat shock protein (Hsp90KDa) is a molecular chaperone involved in the process of cellular oncogenesis, hence its importance as a therapeutic target. Geldanamycin is an inhibitor of Hsp90 chaperone activity, which binds to the ATP binding site in the N-terminal domain of Hsp90. However, geldanamycin has shown hepatotoxic damage in clinical trials; for this reason, its use is not recommended. Taking advantage that geldanamycin binds successfully to Hsp90, many efforts have focused on the search for similar analogues, which have the same or better biological response and reduce the side effects of its predecessor; 17-AAG and 17-DMAG are examples of these analogues. Methods: In order to know the chemical factors influencing the growth or decay of the biological activity of geldanamycin analogues, different computational techniques such as docking, 3DQSAR and quantum similarity were used.  Moreover, the study quantified the interaction energy between amino acids residues of active side and geldanamycin analogues, through hybrid methodology (Autodock-PM6) and DFT indexes. Results: The evaluation of interaction energies showed that the interaction with Lys58 residue is essential for the union of the analogues to the active site of Hsp90, and improves its biological activity. This union is formed through a substituent on C-11 of the geldanamycin macrocycle. A small and attractor group was found as the main steric and electrostatic characteristic that substituents on C11 need in order to interact with Lys 58; behavior was observed with hydroxy and methoxy series of geldanamycin analogues, under study. Conclusion: This study contributes with new hybrid methodology (Autodock-PM6) for the generation of 3DQSAR models, which to consider the interactions between compounds and amino acids residues of Hsp90´s active site in the alignment generation. Additionally, quantum similarity and reactivity indices calculations using DFT were performed to know the non-covalent stabilization in the active site of these compounds.