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J Cell Sci ; 126(Pt 14): 2997-3009, 2013 Jul 15.
Article in English | MEDLINE | ID: mdl-23613469

ABSTRACT

Mating yeast cells interpret complex pheromone gradients and polarize their growth in the direction of the closest partner. Chemotropic growth depends on both the pheromone receptor and its associated G-protein. Upon activation by the receptor, Gα dissociates from Gßγ and Gß is subsequently phosphorylated. Free Gßγ signals to the nucleus via a MAPK cascade and recruits Far1-Cdc24 to the incipient growth site. It is not clear how the cell establishes and stabilizes the axis of polarity, but this process is thought to require local signal amplification via the Gßγ-Far1-Cdc24 chemotropic complex, as well as communication between this complex and the activated receptor. Here we show that a mutant form of Gß that cannot be phosphorylated confers defects in directional sensing and chemotropic growth. Our data suggest that phosphorylation of Gß plays a role in localized signal amplification and in the dynamic communication between the receptor and the chemotropic complex, which underlie growth site selection and maintenance.


Subject(s)
Chemotaxis , GTP-Binding Protein alpha Subunits/metabolism , GTP-Binding Protein beta Subunits/metabolism , Saccharomyces cerevisiae/physiology , Aldehyde Oxidoreductases/metabolism , Cell Cycle Proteins/metabolism , Cell Polarity/genetics , Cyclin-Dependent Kinase Inhibitor Proteins/metabolism , GTP-Binding Protein beta Subunits/genetics , Guanine Nucleotide Exchange Factors/metabolism , MAP Kinase Signaling System/genetics , Mutation/genetics , Phosphorylation/genetics , Protein Binding , Receptors, Pheromone/metabolism , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae Proteins/metabolism
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