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1.
Am J Hum Genet ; 111(1): 39-47, 2024 Jan 04.
Article in English | MEDLINE | ID: mdl-38181734

ABSTRACT

Craniofacial phenotyping is critical for both syndrome delineation and diagnosis because craniofacial abnormalities occur in 30% of characterized genetic syndromes. Clinical reports, textbooks, and available software tools typically provide two-dimensional, static images and illustrations of the characteristic phenotypes of genetic syndromes. In this work, we provide an interactive web application that provides three-dimensional, dynamic visualizations for the characteristic craniofacial effects of 95 syndromes. Users can visualize syndrome facial appearance estimates quantified from data and easily compare craniofacial phenotypes of different syndromes. Our application also provides a map of morphological similarity between a target syndrome and other syndromes. Finally, users can upload 3D facial scans of individuals and compare them to our syndrome atlas estimates. In summary, we provide an interactive reference for the craniofacial phenotypes of syndromes that allows for precise, individual-specific comparisons of dysmorphology.


Subject(s)
Face , Software , Humans , Facies , Phenotype , Syndrome
2.
Rev. Fac. Med. Hum ; 23(2)abr. 2023.
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1514800

ABSTRACT

La perforación gástrica neonatal es una patología rara pero potencialmente mortal; su recurrencia es aún más rara, son escasos los reportes en la literatura internacional. Se presenta el caso de una recién nacida prematura (32 semanas) y bajo peso al nacer (1725g) que el primer día de vida presentó una perforación gástrica corregida quirúrgicamente y al cuarto día postoperatorio es reintervenida con presunción diagnóstica de dehiscencia de rafía gástrica, hallándose una nueva perforación gástrica, la cual fue reparada quirúrgicamente. En ambas ocasiones la intervención quirúrgica fue temprana. La evolución posterior y desenlace fueron favorables.


Neonatal gastric perforation is a rare but potentially fatal pathology; its recurrence is even more infrequent, and there are few reports in the international literature. We present the case of a premature newborn (32 weeks) and low birth weight (1725g) who, on the first day of life, presented a surgically corrected gastric perforation and on the fourth postoperative day was re-operated with a presumed diagnosis of gastric raffia dehiscence, finding a new gastric perforation, which was surgically repaired. On both occasions, the surgical intervention was early. The subsequent evolution and outcome were favorable.

3.
Eur J Hum Genet ; 31(9): 1010-1016, 2023 09.
Article in English | MEDLINE | ID: mdl-36750664

ABSTRACT

Human genetic syndromes are often challenging to diagnose clinically. Facial phenotype is a key diagnostic indicator for hundreds of genetic syndromes and computer-assisted facial phenotyping is a promising approach to assist diagnosis. Most previous approaches to automated face-based syndrome diagnosis have analyzed different datasets of either 2D images or surface mesh-based 3D facial representations, making direct comparisons of performance challenging. In this work, we developed a set of subject-matched 2D and 3D facial representations, which we then analyzed with the aim of comparing the performance of 2D and 3D image-based approaches to computer-assisted syndrome diagnosis. This work represents the most comprehensive subject-matched analyses to date on this topic. In our analyses of 1907 subject faces representing 43 different genetic syndromes, 3D surface-based syndrome classification models significantly outperformed 2D image-based models trained and evaluated on the same subject faces. These results suggest that the clinical adoption of 3D facial scanning technology and continued collection of syndromic 3D facial scan data may substantially improve face-based syndrome diagnosis.


Subject(s)
Face , Image Processing, Computer-Assisted , Humans , Image Processing, Computer-Assisted/methods , Syndrome , Imaging, Three-Dimensional/methods
4.
Artif Intell Med ; 134: 102425, 2022 12.
Article in English | MEDLINE | ID: mdl-36462895

ABSTRACT

Many genetic syndromes are associated with distinctive facial features. Several computer-assisted methods have been proposed that make use of facial features for syndrome diagnosis. Training supervised classifiers, the most common approach for this purpose, requires large, comprehensive, and difficult to collect databases of syndromic facial images. In this work, we use unsupervised, normalizing flow-based manifold and density estimation models trained entirely on unaffected subjects to detect syndromic 3D faces as statistical outliers. Furthermore, we demonstrate a general, user-friendly, gradient-based interpretability mechanism that enables clinicians and patients to understand model inferences. 3D facial surface scans of 2471 unaffected subjects and 1629 syndromic subjects representing 262 different genetic syndromes were used to train and evaluate the models. The flow-based models outperformed unsupervised comparison methods, with the best model achieving an ROC-AUC of 86.3% on a challenging, age and sex diverse data set. In addition to highlighting the viability of outlier-based syndrome screening tools, our methods generalize and extend previously proposed outlier scores for 3D face-based syndrome detection, resulting in improved performance for unsupervised syndrome detection.


Subject(s)
Syndrome , Humans , Databases, Factual
5.
IEEE J Biomed Health Inform ; 26(7): 3229-3239, 2022 07.
Article in English | MEDLINE | ID: mdl-35380975

ABSTRACT

One of the primary difficulties in treating patients with genetic syndromes is diagnosing their condition. Many syndromes are associated with characteristic facial features that can be imaged and utilized by computer-assisted diagnosis systems. In this work, we develop a novel 3D facial surface modeling approach with the objective of maximizing diagnostic model interpretability within a flexible deep learning framework. Therefore, an invertible normalizing flow architecture is introduced to enable both inferential and generative tasks in a unified and efficient manner. The proposed model can be used (1) to infer syndrome diagnosis and other demographic variables given a 3D facial surface scan and (2) to explain model inferences to non-technical users via multiple interpretability mechanisms. The model was trained and evaluated on more than 4700 facial surface scans from subjects with 47 different syndromes. For the challenging task of predicting syndrome diagnosis given a new 3D facial surface scan, age, and sex of a subject, the model achieves a competitive overall top-1 accuracy of 71%, and a mean sensitivity of 43% across all syndrome classes. We believe that invertible models such as the one presented in this work can achieve competitive inferential performance while greatly increasing model interpretability in the domain of medical diagnosis.


Subject(s)
Diagnosis, Computer-Assisted , Face , Diagnosis, Computer-Assisted/methods , Face/diagnostic imaging , Humans
6.
PLoS One ; 15(6): e0233377, 2020.
Article in English | MEDLINE | ID: mdl-32502155

ABSTRACT

The biology of how faces are built and come to differ from one another is complex. Discovering normal variants that contribute to differences in facial morphology is one key to untangling this complexity, with important implications for medicine and evolutionary biology. This study maps quantitative trait loci (QTL) for skeletal facial shape using Diversity Outbred (DO) mice. The DO is a randomly outcrossed population with high heterozygosity that captures the allelic diversity of eight inbred mouse lines from three subspecies. The study uses a sample of 1147 DO animals (the largest sample yet employed for a shape QTL study in mouse), each characterized by 22 three-dimensional landmarks, 56,885 autosomal and X-chromosome markers, and sex and age classifiers. We identified 37 facial shape QTL across 20 shape principal components (PCs) using a mixed effects regression that accounts for kinship among observations. The QTL include some previously identified intervals as well as new regions that expand the list of potential targets for future experimental study. Three QTL characterized shape associations with size (allometry). Median support interval size was 3.5 Mb. Narrowing additional analysis to QTL for the five largest magnitude shape PCs, we found significant overrepresentation of genes with known roles in growth, skeletal and facial development, and sensory organ development. For most intervals, one or more of these genes lies within 0.25 Mb of the QTL's peak. QTL effect sizes were small, with none explaining more than 0.5% of facial shape variation. Thus, our results are consistent with a model of facial diversity that is influenced by key genes in skeletal and facial development and, simultaneously, is highly polygenic.


Subject(s)
Bone Development/genetics , Facial Bones/anatomy & histology , Maxillofacial Development/genetics , Alleles , Animals , Bone and Bones/anatomy & histology , Chromosome Mapping/methods , Collaborative Cross Mice/genetics , Face/anatomy & histology , Female , Genetic Variation/genetics , Genotype , Male , Mice , Phenotype , Polymorphism, Single Nucleotide/genetics , Quantitative Trait Loci/genetics
7.
Sensors (Basel) ; 20(11)2020 Jun 03.
Article in English | MEDLINE | ID: mdl-32503190

ABSTRACT

3D facial landmarks are known to be diagnostically relevant biometrics for many genetic syndromes. The objective of this study was to extend a state-of-the-art image-based 2D facial landmarking algorithm for the challenging task of 3D landmark identification on subjects with genetic syndromes, who often have moderate to severe facial dysmorphia. The automatic 3D facial landmarking algorithm presented here uses 2D image-based facial detection and landmarking models to identify 12 landmarks on 3D facial surface scans. The landmarking algorithm was evaluated using a test set of 444 facial scans with ground truth landmarks identified by two different human observers. Three hundred and sixty nine of the subjects in the test set had a genetic syndrome that is associated with facial dysmorphology. For comparison purposes, the manual landmarks were also used to initialize a non-linear surface-based registration of a non-syndromic atlas to each subject scan. Compared to the average intra- and inter-observer landmark distances of 1.1 mm and 1.5 mm respectively, the average distance between the manual landmark positions and those produced by the automatic image-based landmarking algorithm was 2.5 mm. The average error of the registration-based approach was 3.1 mm. Comparing the distributions of Procrustes distances from the mean for each landmarking approach showed that the surface registration algorithm produces a systemic bias towards the atlas shape. In summary, the image-based automatic landmarking approach performed well on this challenging test set, outperforming a semi-automatic surface registration approach, and producing landmark errors that are comparable to state-of-the-art 3D geometry-based facial landmarking algorithms evaluated on non-syndromic subjects.


Subject(s)
Face , Genetic Diseases, Inborn/diagnostic imaging , Imaging, Three-Dimensional , Algorithms , Face/diagnostic imaging , Humans
8.
Genet Med ; 22(10): 1682-1693, 2020 10.
Article in English | MEDLINE | ID: mdl-32475986

ABSTRACT

PURPOSE: Deep phenotyping is an emerging trend in precision medicine for genetic disease. The shape of the face is affected in 30-40% of known genetic syndromes. Here, we determine whether syndromes can be diagnosed from 3D images of human faces. METHODS: We analyzed variation in three-dimensional (3D) facial images of 7057 subjects: 3327 with 396 different syndromes, 727 of their relatives, and 3003 unrelated, unaffected subjects. We developed and tested machine learning and parametric approaches to automated syndrome diagnosis using 3D facial images. RESULTS: Unrelated, unaffected subjects were correctly classified with 96% accuracy. Considering both syndromic and unrelated, unaffected subjects together, balanced accuracy was 73% and mean sensitivity 49%. Excluding unrelated, unaffected subjects substantially improved both balanced accuracy (78.1%) and sensitivity (56.9%) of syndrome diagnosis. The best predictors of classification accuracy were phenotypic severity and facial distinctiveness of syndromes. Surprisingly, unaffected relatives of syndromic subjects were frequently classified as syndromic, often to the syndrome of their affected relative. CONCLUSION: Deep phenotyping by quantitative 3D facial imaging has considerable potential to facilitate syndrome diagnosis. Furthermore, 3D facial imaging of "unaffected" relatives may identify unrecognized cases or may reveal novel examples of semidominant inheritance.


Subject(s)
Face , Imaging, Three-Dimensional , Face/diagnostic imaging , Humans , Syndrome
9.
Space Sci Rev ; 214(1)2018 02.
Article in English | MEDLINE | ID: mdl-30713357

ABSTRACT

OSIRIS-REx will return pristine samples of carbonaceous asteroid Bennu. This article describes how pristine was defined based on expectations of Bennu and on a realistic understanding of what is achievable with a constrained schedule and budget, and how that definition flowed to requirements and implementation. To return a pristine sample, the OSIRIS-REx spacecraft sampling hardware was maintained at level 100 A/2 and <180 ng/cm2 of amino acids and hydrazine on the sampler head through precision cleaning, control of materials, and vigilance. Contamination is further characterized via witness material exposed to the spacecraft assembly and testing environment as well as in space. This characterization provided knowledge of the expected background and will be used in conjunction with archived spacecraft components for comparison with the samples when they are delivered to Earth for analysis. Most of all, the cleanliness of the OSIRIS-REx spacecraft was achieved through communication among scientists, engineers, managers, and technicians.

11.
N Engl J Med ; 373(21): 2025-2037, 2015 Nov 19.
Article in English | MEDLINE | ID: mdl-26488565

ABSTRACT

BACKGROUND: The RTS,S/AS01 vaccine targets the circumsporozoite protein of Plasmodium falciparum and has partial protective efficacy against clinical and severe malaria disease in infants and children. We investigated whether the vaccine efficacy was specific to certain parasite genotypes at the circumsporozoite protein locus. METHODS: We used polymerase chain reaction-based next-generation sequencing of DNA extracted from samples from 4985 participants to survey circumsporozoite protein polymorphisms. We evaluated the effect that polymorphic positions and haplotypic regions within the circumsporozoite protein had on vaccine efficacy against first episodes of clinical malaria within 1 year after vaccination. RESULTS: In the per-protocol group of 4577 RTS,S/AS01-vaccinated participants and 2335 control-vaccinated participants who were 5 to 17 months of age, the 1-year cumulative vaccine efficacy was 50.3% (95% confidence interval [CI], 34.6 to 62.3) against clinical malaria in which parasites matched the vaccine in the entire circumsporozoite protein C-terminal (139 infections), as compared with 33.4% (95% CI, 29.3 to 37.2) against mismatched malaria (1951 infections) (P=0.04 for differential vaccine efficacy). The vaccine efficacy based on the hazard ratio was 62.7% (95% CI, 51.6 to 71.3) against matched infections versus 54.2% (95% CI, 49.9 to 58.1) against mismatched infections (P=0.06). In the group of infants 6 to 12 weeks of age, there was no evidence of differential allele-specific vaccine efficacy. CONCLUSIONS: These results suggest that among children 5 to 17 months of age, the RTS,S vaccine has greater activity against malaria parasites with the matched circumsporozoite protein allele than against mismatched malaria. The overall vaccine efficacy in this age category will depend on the proportion of matched alleles in the local parasite population; in this trial, less than 10% of parasites had matched alleles. (Funded by the National Institutes of Health and others.).


Subject(s)
Malaria Vaccines/immunology , Malaria, Falciparum/prevention & control , Plasmodium falciparum/genetics , Africa , Female , Genetic Variation , Humans , Infant , Malaria, Falciparum/immunology , Malaria, Falciparum/parasitology , Male , Treatment Outcome
12.
Tech Coloproctol ; 18(2): 173-8, 2014 Feb.
Article in English | MEDLINE | ID: mdl-23686679

ABSTRACT

BACKGROUND: Surgical management of complete rectal prolapse is challenging. We present our results with the novel technique stapled transanal longitudinal posterior proctectomy (STALPP) in patients with complete rectal prolapse. METHODS: We performed a retrospective study in two hospitals from January 2005 to December 2012. Twenty-one patients with complete rectal prolapse were included. In all patients, STALPP was performed. The study variables were operative time, intraoperative bleeding, number of cartridges used, length of rectum prolapsed through the anus, length of rectal wall resected, length of hospital stay and preoperative and postoperative Wexner continence score and manometric measurement of anal canal resting tone and squeeze pressure. RESULTS: The median length of prolapsed tissue was 13 cm; the mean Wexner score in the preoperative and postoperative period was 15.95 and 4.95, respectively (p = 0.025). The mean resting tone improved from 23.3 to 32.85 mmHg postoperatively (p = 0.03), as did maximal squeeze pressure from 31 to 62.7 mmHg (p = 0.003). Median operative time was 65 min; median intraoperative bleeding was 12 ml; there was no postoperative bleeding, and no reinterventions were required. The median number of cartridges used was 4. The median length of resected wall in the right posterolateral sector was 8 and 6 cm in the left. The median length of hospital stay was 4 days, and the mean follow-up period was 2 years. No mortality was reported. CONCLUSIONS: Stapled transanal longitudinal posterior proctectomy is a safe and feasible surgical alternative for patients with complete rectal prolapse.


Subject(s)
Digestive System Surgical Procedures/methods , Rectal Prolapse/surgery , Surgical Stapling , Adult , Aged , Aged, 80 and over , Anal Canal/physiopathology , Blood Loss, Surgical , Fecal Incontinence/etiology , Female , Humans , Length of Stay , Male , Manometry , Middle Aged , Operative Time , Rectal Prolapse/complications , Rectal Prolapse/pathology , Retrospective Studies , Young Adult
13.
Rev Gastroenterol Mex ; 77(1): 9-14, 2012.
Article in Spanish | MEDLINE | ID: mdl-22450015

ABSTRACT

INTRODUCTION: Postoperative pain after open cholecystectomy is associated with reduced respiratory function, longer recovery period before deambulation and oral food intake, and prolonged hospital stay. Intercostal nerve block provides satisfactory analgesia and ropivacaine is the most widely used local anesthetic agent in intercostal nerve block due to its excellent effectiveness, lower cardiovascular toxicity, and longer half-life. AIMS: To evaluate intercostal nerve block effectiveness with ropivacaine in patients undergoing emergency open cholecystectomy under general anesthesia compared with conventional management. MATERIAL AND METHODS: A controlled clinical trial was carried out on 50 patients undergoing open cholecystectomy, 25 patients without intercostal nerve block versus 25 patients with intercostal nerve block using ropivacaine at 0.5% combined with epinephrine. Intraoperative minimum alveolar concentration and inhalation anesthetic use were evaluated. Tramadol as rescue analgesic agent and pain were evaluated during immediate postoperative period by means of the Visual Analog Scale at 8, 16, and 24 hours. RESULTS: Mean inhalation anesthetic use was lower in the intercostal nerve block group with 13% vs 37% in the group without intercostal nerve block (p= 0.01). Rescue tramadol requirement was lower in the intercostal nerve block group than in the group without intercostal nerve block at 8 hours (8% vs 67%), 16 hours (0% vs 83%), and 24 hours (12% vs 79%) (p<0.0001). Visual Analog Scale for Pain results were similar in both groups. CONCLUSIONS: Intercostal nerve block reduces intraoperative inhalation anesthetic use, immediate postoperative pain, and tramadol intake as rescue analgesic agent in patients undergoing open cholecystectomy.


Subject(s)
Amides/therapeutic use , Analgesia/methods , Anesthesia, General , Anesthetics, Local/therapeutic use , Cholecystectomy , Intercostal Nerves , Nerve Block/methods , Pain Management/methods , Pain, Postoperative/prevention & control , Adolescent , Adult , Aged , Cholecystectomy/methods , Cross-Sectional Studies , Emergency Treatment , Female , Humans , Male , Middle Aged , Prospective Studies , Ropivacaine , Young Adult
14.
Pharmacogenomics J ; 12(5): 425-31, 2012 Oct.
Article in English | MEDLINE | ID: mdl-21606947

ABSTRACT

Elevated plasma levels of lipoprotein-associated phospholipase A(2) (Lp-PLA2) activity have been shown to be associated with increased risk of coronary heart disease and an inhibitor of this enzyme is under development for the treatment of that condition. A Val279Phe null allele in this gene, that may influence patient eligibility for treatment, is relatively common in East Asians but has not been observed in Europeans. We investigated the existence and functional effects of low frequency alleles in a Western European population by re-sequencing the exons of PLA2G7 in 2000 samples. In all, 19 non-synonymous single-nucleotide polymorphisms (nsSNPs) were found, 14 in fewer than four subjects (minor allele frequency <0.1%). Lp-PLA2 activity was significantly lower in rare nsSNP carriers compared with non-carriers (167.8±63.2 vs 204.6±41.8, P=0.01) and seven variants had enzyme activities consistent with a null allele. The cumulative frequency of these null alleles was 0.25%, so <1 in 10,000 Europeans would be expected to be homozygous, and thus not potentially benefit from treatment with an Lp-PLA2 inhibitor.


Subject(s)
Amino Acid Substitution/genetics , Coronary Disease/genetics , Mutation , Phospholipases A2/genetics , 1-Alkyl-2-acetylglycerophosphocholine Esterase , Alleles , Coronary Disease/drug therapy , Enzyme Inhibitors/therapeutic use , Genetics, Population , Homozygote , Humans , Phospholipase A2 Inhibitors , Phospholipases A2/metabolism , Polymorphism, Single Nucleotide , Sequence Analysis, DNA , White People/genetics
15.
Mol Vis ; 17: 3137-46, 2011.
Article in English | MEDLINE | ID: mdl-22171160

ABSTRACT

PURPOSE: The corneal wound healing response to an alkali burn results in dysregulated inflammation and opacity. Transient receptor potential vanilloid type1 (TRPV1) ion channel activation by such a stress contributes to this unfavorable outcome. Accordingly, we sought to identify potential drug targets for mitigating this response, in human corneal epithelial cells (HCEC). METHODS: SV40-immmortalized HCEC were transduced with lentiviral vectors to establish stable c-Jun N-terminal kinase1 (JNK1), nuclear factor-κB1 (NF-κB1), and dual specificity phsophatase1 (DUSP1) shRNAmir sublines. Immunoblotting evaluated the expression of NF-κB1, DUSP1, protein kinase Cδ (PKCδ), and the phosphorylation status of cell signaling mediators. Enzyme-linked immunosorbent assay (ELISA) evaluated interleukin-6 (IL-6) and interleukin-8 (IL-8) release. RESULTS: Capsaicin (CAP; a selective TRPV1 agonist), induced time-dependent activation of transforming growth factor-activated kinase 1 (TAK1) and mitogen-activated protein kinase (MAPK) cascades temporally followed by increased nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα) phosphorylation, rises in both PKCδ protein levels and IL-6 and IL-8 release. All of these responses were blocked by the TAK1 inhibitor 5z-7-oxozeaenol (5z-OX). In the JNK1 subline, CAP failed to increase IL-6/8 release, but still stimulated NF-κB by 50%. In the NF-κB1 subline, these IL-6/8 responses were absent, JNK1 activation was attenuated and there was a concomitant increase in DUSP1 expression compared to the control. In the DUSP1 subline, JNK1 phosphorylation was enhanced and prolonged and accompanied by larger increases in IL-6/8 release. CONCLUSIONS: TRPV1 induced increases in IL-6/IL-8 release occur through TAK1 activation of JNK1-dependent and JNK1-independent signaling pathways. Their joint activation is required for NF-κB to elicit sufficient positive feedback control of JNK1/2 phosphorylation to elicit increases in IL-6/8 release. Such regulation depends on NF-κB modulation of DUSP1 expression levels and associated changes in PKCδ protein levels.


Subject(s)
Epithelial Cells/enzymology , Feedback, Physiological , Interleukin-6/metabolism , Interleukin-8/metabolism , Mitogen-Activated Protein Kinase 8/metabolism , NF-kappa B/metabolism , TRPV Cation Channels/metabolism , Capsaicin/pharmacology , Dual Specificity Phosphatase 1/metabolism , Enzyme Activation/drug effects , Epithelial Cells/drug effects , Epithelium, Corneal/cytology , Feedback, Physiological/drug effects , Humans , MAP Kinase Kinase Kinases/metabolism , MAP Kinase Signaling System/drug effects , Phosphorylation/drug effects , Protein Kinase C-delta/metabolism
16.
Rev Gastroenterol Mex ; 76(3): 251-4, 2011.
Article in Spanish | MEDLINE | ID: mdl-22041316

ABSTRACT

Heteropropic ossification was initially described by Riedel in 1883. This is a rare disease but well documented in gastrointestinal tract. Several mechanisms have been proposed to determine the etiology of this metaplasia. We present a 59 year old male that was treated in the National Institute of Medicine Sciences and Nutrition Salvador Zubirán in whom after various abdominal surgical procedures the presence of bowel osseous metaplasia was documented.


Subject(s)
Ileostomy , Intestine, Small/pathology , Ossification, Heterotopic/pathology , Postoperative Complications/pathology , Abdomen/surgery , Humans , Male , Mesentery , Metaplasia , Middle Aged
18.
HIV Med ; 12(8): 500-5, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21794055

ABSTRACT

INTRODUCTION: The collection of incidence data on HIV infection is necessary to evaluate the status and dynamics of the epidemic and the effectiveness of intervention strategies. However, this is usually difficult in low-income countries. METHODS: Five yearly point HIV prevalence estimations (in 1999, 2003, 2004, 2005 and 2008) were obtained for women between 15 and 45 years of age participating in three studies carried out for other purposes at the Antenatal Clinic (ANC) in Manhiça, Mozambique. HIV incidence was estimated between prevalence points using a previously validated methodology. Two methods were used, one based on mortality rates for three HIV epidemic scenarios, and the other based on survival information after infection. The pattern over time was captured by fitting a log-regression model. RESULTS: The prevalence of HIV infection ranged from 12% in 1999 to 49% in 2008. The HIV incidence increased from approximately 3.5 cases per 100 person-years in 2001 to 14 per 100 person-years in 2004, with stabilization thereafter to levels of around 12 cases per 100 person-years. The incidence estimates were comparable for the two methods used. CONCLUSION: These findings indicate an increase in the prevalence and incidence of HIV infection among women of reproductive age over the 9 years of the analysis, with a plateau in the incidence of infection since 2005. However, the very high figures for both prevalence and incidence highlight the importance of the continuation of the prevention and treatment programmes that already exist, and suggest that implementation of preventive measures is needed in this area.


Subject(s)
HIV Infections/epidemiology , Adolescent , Adult , Developing Countries/statistics & numerical data , Female , Humans , Incidence , Logistic Models , Middle Aged , Mozambique/epidemiology , Pregnancy , Prevalence , Rural Population , Young Adult
19.
Rev Gastroenterol Mex ; 75(4): 369-73, 2010.
Article in Spanish | MEDLINE | ID: mdl-21169102

ABSTRACT

BACKGROUND AND AIM: Disturbances in intestinal motility have been described since XIX century, with a not very well understood pathogenesis and few therapeutic approaches. Considering chewing as an important stimulus to promote intestinal motility we designed this study to assess the efficacy of chewing gum to improve the postoperative ileus, and the clinical relevance of this intervention. METHODS: Were included patients who underwent for elective left hemicolectomy during January 2007 to December 2008. The patients were randomly assigned to receive chewing gum or nothing during the post-surgery period. The main outcomes assessed were duration of postoperative ileus, and reduction in hospitalization days. RESULTS: During the period of the study were included 32 patients, were included mainly men (11 and 9 in treated and control patients, respectively). The first defecation after surgery procedure occurs at (44.2 ± 17.6 h) in the treated group and (55.5 ± 14.8 h) in the control group (p = 0.05). In the same way oral tolerance was achieved faster in the treated group (p= 0.05). The hospital stay duration was not different among treated and not treated patients. CONCLUSIONS: The use of chewing gum in patients after elective left hemicolectomy, reduce the duration of the postoperative ileus. However, this does not reduce the hospital stay duration.


Subject(s)
Chewing Gum , Colectomy , Ileus/therapy , Postoperative Complications/therapy , Adult , Aged , Aged, 80 and over , Female , Humans , Length of Stay , Male , Middle Aged
20.
Exp Eye Res ; 91(3): 462-71, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20619260

ABSTRACT

Corneal epithelial injury induces release of endogenous metabolites that are cannabinoid receptor 1 (CB1) and transient receptor potential vanilloid 1 (TRPV1) agonists. We determined the functional contributions by CB1 and TRPV1 activation to eliciting responses underlying wound healing in human corneal epithelial cells (HCEC). Both the selective CB1 and TRPV1 agonists (i.e., WIN55,212-2 [WIN] and capsaicin [CAP], respectively) induced EGFR phosphorylation whereas either inhibition of its tyrosine kinase activity with AG1478 or functional blockage eliminated this response. Furthermore, EGFR transactivation was abolished by inhibitors of proteolytic release of heparin bound EGF (HB-EGF). CB1-induced Ca(2+) transients were reduced during exposure to either the CB1 antagonist, AM251 or AG1478. Both CAP and WIN induced transient increases in Erk1/2, p38, JNK1/2 MAPK and Akt/PI-3K phosphorylation status resulting in cell proliferation and migration increases which mirrored those elicited by EGF. Neither EGF nor WIN induced any increases in IL-6 and IL-8 release. On the other hand, CAP-induced 3- and 6-fold increases, which were fully attenuated during exposure to CPZ, but AG1478 only suppressed them by 21%. The mixed CB1 and TRPV1 antagonist, AM251, enhanced the CAP-induced rise in IL-8 release to a higher level than that elicited by CAP alone. In conclusion, CB1 and TRPV1 activation induces increases in HCEC proliferation and migration through EGFR transactivation leading to global MAPK and Akt/PI-3K pathway stimulation. On the other hand, the TRPV1-mediated increases in IL-6 and IL-8 release are elicited through both EGFR dependent and EGFR-independent signaling pathways.


Subject(s)
Epithelium, Corneal/metabolism , ErbB Receptors/metabolism , Receptor, Cannabinoid, CB1/metabolism , TRPV Cation Channels/metabolism , Transcriptional Activation/physiology , Benzoxazines/pharmacology , Blotting, Western , Capsaicin/pharmacology , Cell Movement , Cell Proliferation , Cells, Cultured , Enzyme-Linked Immunosorbent Assay , Epithelium, Corneal/drug effects , ErbB Receptors/antagonists & inhibitors , Fluorescent Antibody Technique, Indirect , Humans , Interleukin-6/metabolism , Interleukin-8/metabolism , Mitogen-Activated Protein Kinase 1/metabolism , Mitogen-Activated Protein Kinase 3/metabolism , Mitogen-Activated Protein Kinase 8/metabolism , Mitogen-Activated Protein Kinase 9/metabolism , Morpholines/pharmacology , Naphthalenes/pharmacology , Phosphorylation , Piperidines/pharmacology , Pyrazoles/pharmacology , Quinazolines , Receptor, Cannabinoid, CB1/antagonists & inhibitors , TRPV Cation Channels/antagonists & inhibitors , Tyrphostins/pharmacology , p38 Mitogen-Activated Protein Kinases/metabolism
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