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FEBS Lett ; 519(1-3): 30-4, 2002 May 22.
Article in English | MEDLINE | ID: mdl-12023013

ABSTRACT

Ripe tomato fruits accumulate significant amounts of the linear carotene lycopene, but only trace amounts of xanthophylls (oxygenated carotenoids). We overexpressed the lycopene beta-cyclase (b-Lcy) and beta-carotene hydroxylase (b-Chy) genes under the control of the fruit-specific Pds promoter. Transgene and protein expression was followed through semi-quantitative reverse transcription-PCR, Western blotting, and enzyme assays. Fruits of the transformants showed a significant increase of beta-carotene, beta-cryptoxanthin and zeaxanthin. The carotenoid composition of leaves remained unaltered. The transgenes and the phenotype are inherited in a dominant Mendelian fashion. This is the first example of successful metabolic engineering of xanthophyll content in tomato fruits.


Subject(s)
Genetic Engineering/methods , Intramolecular Lyases/genetics , Mixed Function Oxygenases/genetics , Solanum lycopersicum/genetics , Solanum lycopersicum/metabolism , Xanthophylls/metabolism , beta Carotene/analogs & derivatives , Arabidopsis/genetics , Blotting, Western , Cryptoxanthins , Fruit/chemistry , Fruit/metabolism , Gene Expression , Genes, Dominant , Intramolecular Lyases/metabolism , Solanum lycopersicum/chemistry , Mixed Function Oxygenases/metabolism , Phenotype , Plant Leaves/chemistry , Plant Leaves/metabolism , Plants, Genetically Modified , Reverse Transcriptase Polymerase Chain Reaction , Transgenes , Xanthophylls/analysis , Zeaxanthins , beta Carotene/analysis , beta Carotene/metabolism
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