Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
BMC Neurosci ; 14: 92, 2013 Aug 30.
Article in English | MEDLINE | ID: mdl-23987566

ABSTRACT

BACKGROUND: Sleep restriction alters pain perception in animals and humans, and many studies have indicated that paradoxical sleep deprivation (PSD) promotes hyperalgesia. The hyperalgesia observed after mechanical nociceptive stimulus is reversed through nitric oxide synthase (NOS) inhibition. Both nitric oxide (NO) and the dorsolateral periaqueductal gray matter (dlPAG) area of the brainstem are involved in hyperalgesia. Thus, in this work, we investigated the pain-related behavior response after mechanical noxious stimuli (electronic von Frey test), and the activity of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d), an indicator of NOS activity, within the dlPAG of paradoxical sleep-deprived rats. We also evaluated the effects of pre-treatment with L-NAME on these parameters. RESULTS: These data revealed that PSD reduced the hindpaw withdrawal threshold (-47%, p < 0.0001) confirming the hyperalgesic effect of this condition. In addition, there were more NADPH-d positive cells in dlPAG after PSD than in control rats (+ 59%, p < 0.0001). L-NAME treatment prevented the reduction in the hindpaw withdrawal threshold (+ 93%, p < 0.0001) and the increase in the NADPH-d positive cells number in the dlPAG of PSD-treated rats (-36%, p < 0.0001). CONCLUSION: These data suggest that the hyperalgesic response to mechanical noxious stimuli in paradoxical sleep-deprived rats is associated with increased NOS activity in the dlPAG, which presumably influences the descending antinociceptive pathway.


Subject(s)
Hyperalgesia/metabolism , Nitric Oxide/metabolism , Periaqueductal Gray/metabolism , Sleep Deprivation/metabolism , Animals , Male , Physical Stimulation , Rats , Rats, Wistar
2.
Pharmacol Biochem Behav ; 94(1): 51-5, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19619573

ABSTRACT

Sleep deprivation has been associated with hyperalgesia in humans and in animal models. The tricyclic antidepressant amitriptyline is used as an analgesic drug in patients and in animal models of chronic pain, including that associated with spinal nerve injury. Pain hypersensitivity following paradoxical sleep deprivation (PSD) and that following peripheral nerve injury seem to share common spinal mechanisms. Accordingly, we evaluated the effects of amitriptyline (acutely and chronically administered) on the increased thermal response observed in PSD rats (72 or 96 h). Rats were evaluated for thermal sensitivity using a hot plate (52 degrees C or 46 degrees C) at 1 or 24 h after the last administration of the drug. Following the hot plate test, motor behavior was analyzed in an open field arena for a period of 5 min. Paw withdrawal latency response to temperatures of 46 degrees C and 52 degrees C was significantly lower in PSD and in 24-hour post-PSD rats than in controls and it was not modified by amitriptyline (3, 10 and 30 mg/kg). Analgesic effects and reduced motor behavior were only observed in control groups. Overall, these findings indicate that a period of PSD can influence pain modulatory mechanisms, and that amitriptyline action is insufficient to reduce PSD-enhanced thermal sensitivity.


Subject(s)
Amitriptyline/therapeutic use , Analgesics, Non-Narcotic/therapeutic use , Body Temperature Regulation/drug effects , Hyperalgesia/etiology , Sleep Deprivation/complications , Amitriptyline/administration & dosage , Analgesics, Non-Narcotic/administration & dosage , Analysis of Variance , Animals , Behavior, Animal/drug effects , Hindlimb , Hot Temperature , Hyperalgesia/drug therapy , Locomotion/drug effects , Male , Pain Measurement , Pain Threshold/drug effects , Rats , Rats, Wistar , Reaction Time/drug effects , Time Factors
SELECTION OF CITATIONS
SEARCH DETAIL
...