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Evaluation of MALDI-TOF MS as a tool for detection of Listeria spp. directly from selective enrichment broth from food and stool samples.

Araújo, Thais Martins Campos; Pereira, Rodrigo de Castro Lisbôa; Freitag, Isabelle Geoffroy Ribeiro; Rusak, Leonardo Alves; Botelho, Larissa Alvarenga Batista; Hofer, Ernesto; Hofer, Cristina Barroso; Vallim, Deyse Christina.

J Microbiol Methods ; 173: 105936, 2020 06.

Article in English | MEDLINE | ID: mdl-32360382

ABSTRACT

We evaluated the detection of Listeria spp. using MALDI-TOF MS directly in enrichment broths, without isolated colonies, with naturally contaminated food and stool samples. The success rate was 77%. Considering the reduced time for diagnosis and the success rate, this is a promising screening tool, but more tests are needed to determine its viability.

Subject(s)

Bacteriological Techniques/methods , Feces/microbiology , Food Microbiology/methods , Listeria/isolation & purification , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , Algorithms , Brazil , Culture Media , Food , Foodborne Diseases/diagnosis , Foodborne Diseases/microbiology , Humans , Listeria monocytogenes/isolation & purification

ABSTRACT

Subject(s)

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