ABSTRACT
The effect of 10 days of interval hypoxic training (IHT) on the mRNA expression and protein content of antioxidant enzymes (superoxide dismutase, catalase and glutathione-S-transferase) in leukocytes of children with bronchial asthma (BA) was investigated. It was shown that after sessions of IHT the mRNA expression of superoxide dismutase decreased by 32.5% (P < 0,05), but the level of protein was unchanged. The level of catalase gene mRNA and protein content in leukocytes after IHT increased by 67% and 13% accordingly. We detected a 90% increase in glutathione S-transferase level following IHT.
Subject(s)
Adaptation, Physiological/physiology , Antioxidants/metabolism , Asthma/therapy , Hypoxia/physiopathology , RNA, Messenger/biosynthesis , Adolescent , Asthma/blood , Asthma/enzymology , Asthma/physiopathology , Catalase/biosynthesis , Catalase/genetics , Child , Female , Gene Expression , Glutathione Transferase/biosynthesis , Glutathione Transferase/genetics , Humans , Hypoxia/enzymology , Leukocytes/enzymology , Male , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain Reaction , Superoxide Dismutase/biosynthesis , Superoxide Dismutase/genetics , Treatment OutcomeABSTRACT
Disorders found by Spirography have been analysed. These data were obtained from 137 children with bronchial asthma and with wheezing syndrome and who live in ecologically not unfavorable regions of Ukraine. Decrease in patency of airways of small and big caliber was detected in patients with bronchial asthma from Dneprodzerzhinsk and the middle and Decrease in patency of airways of middle and small caliber small caliber of patients from Mariupol. The pharmacological test with salbuthamol demonstrated considerable changes in the reactivity of bronchial apparatus that shows necessity of administration of drugs correction in children with bronchial asthma and necessity to detail indications for this correction to patients with an isolated decreased patency of airways of big caliber.
