[The influence of extracorporeal laser radiation on structural indices of erythrocytes].

Object of the research was to study the diffractometric indices of erythrocytes, while 1 ml of the blood of the experimental animals was irradiated extracorporally by helium-neon laser. For this purpose 1 ml blood was taken from normal weight, (1200 gr) grown up shinshila rabbits, that we divided into 7 groups and irradiated with 10 vat helium-neon laser during 0.5-1 minutes. After irradiation blood was injected back to the organism of rabbits. After 2-6 hours from irradiation blood was taken from veins, to study by electronic microscope and later to be processed by diffractometric analysis method. The examinations testify that after extracorporeal irradiation diffractometric characteristics of erythrocytes' membranes are lower than after general irradiation, which indicates to the different energetic possibilities of laser. The extracorporeal irradiation, performed by laser and input of radiated blood back to organism is considered to be a shock therapy from the side of erythrocytes, which promote the defense function of the organism itself. The base for the shock therapy should be important factors such as homeostasis, compensative-adaptive mechanisms and so on. Exactly this above mentioned should be manifested after the sensitized cells are led back to the body (1 ml of blood) and with their existence they should promote display of the defense mechanisms.

[Age related changes of physical-chemical parameters of erythrocytes].

The aim of our study was to investigate age related changes of physical-chemical parameters of erythrocytes. It is known that insufficiency of erythrocytes antioxidant defence system in newborns is a keystone of peculiarities manifested in homeostasis among this group. The excess volume of reactive oxygen determines disorders related to the functioning of cation channels that are represented by decrease of K(+) ions, moderate hydration of erythrocytes and may cause accumulation of intraerythrocyte calcium. Excessive calcium may also cause activation of Ca(2+)-dependent erythrocytes' NOS and increase of nitric oxide content in newborns' erythrocytes. However, in spite of high NO content, the insufficiency of antioxidant system is conductive to intensive formation of methaemoglobin in newborns' blood. The other cause of intensive haemolysis of erythrocytes in this age group may be also the activation of Ca(2+)-dependent mechanism, which contributes to the exposition of phosphatidilserin on the surface of the erythrocyte's membrane and elimination of erythrocytes by macrophages. The increasing activity of antioxidant enzymes among aged persons seems to be a compensatory mechanism against excessively reactive oxygen in old age and this process ensures maintenance of intensity of hemolysis within the physiological frames.