ABSTRACT
This technique has been developed for the estimation of the infectivity of herpes simplex virus type I passage III on a tissue culture of Vero and HBK21. The plaque assay method was performed and the number of plaques on the two culture media was counted per each dilution from (10(-5) to 10(-8)). It was found that the number and the size of plaques in 10(-5) dilution are the best in the two types of tissue cultures. The number indicates the number of virus particle (per each dilution point), which can be used for infection of experimental animals to demonstrate its infectivity character.
Subject(s)
Simplexvirus/growth & development , Viral Plaque Assay/methods , Animals , Colony Count, Microbial , Culture Techniques , Virus CultivationABSTRACT
The Cunningham and Sezenberg Technique (using mice sensitized lymphoid cells to SRBCs) was tested together with Trump's gel technique (using non sensitized mice lymphoid cell mixed with SRBCs and incubated in Marbrook culture chamber for sensitization). The study showed that the number of PFCs by the Cunningham slid was greater than that observed by the gel technique of Trump at the same lymphocyte concentration. Also it was observed that the Trump's method using the Marbrook tissue culture chamber was a time consuming technique than that obtained by the Cunningham technique.
