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1.
Neuroscience ; 306: 18-27, 2015 Oct 15.
Article in English | MEDLINE | ID: mdl-26283024

ABSTRACT

INTRODUCTION: Neuro-vascular rearrangement occurs in brain disorders, including epilepsy. Platelet-derived growth factor receptor beta (PDGFRß) is used as a marker of perivascular pericytes. Whether PDGFRß(+) cell reorganization occurs in regions of neuro-vascular dysplasia associated with seizures is unknown. METHODS: We used brain specimens derived from epileptic subjects affected by intractable seizures associated with focal cortical dysplasia (FCD) or temporal lobe epilepsy with hippocampal sclerosis (TLE-HS). Tissues from cryptogenic epilepsy, non-sclerotic hippocampi or peritumoral were used for comparison. An in vivo rat model of neuro-vascular dysplasia was obtained by pre-natal exposure to methyl-axozy methanoic acid (MAM). Status epilepticus (SE) was induced in adult MAM rats by intraperitoneal pilocarpine. MAM tissues were also used to establish organotypic hippocampal cultures (OHC) to further assess pericytes positioning at the dysplastic microvasculature. PDGFRß and its colocalization with RECA-1 or CD34 were used to segregate perivascular pericytes. PDGFRß and NG2 or IBA1 colocalization were performed. Rat cortices and hippocampi were used for PDGFRß western blot analysis. RESULTS: Human FCD displayed the highest perivascular PDGFRß immunoreactivity, indicating pericytes, and presence of ramified PDGFRß(+) cells in the parenchyma and proximal to microvessels. Tissues deriving from human cryptogenic epilepsy displayed a similar pattern of immunoreactivity, although to a lesser extent compared to FCD. In TLE-HS, CD34 vascular proliferation was paralleled by increased perivascular PDGFRß(+) pericytes, as compared to non-HS. Parenchymal PDGFRß immunoreactivity co-localized with NG2 but was distinct from IBA1(+) microglia. In MAM rats, we found pericyte-vascular changes in regions characterized by neuronal heterotopias. PDGFRß immunoreactivity was differentially distributed in the heterotopic and adjacent normal CA1 region. The use of MAM OHC revealed microvascular-pericyte dysplasia at the capillary tree lining the dentate gyrus (DG) molecular layer as compared to control OHC. Severe SE induced PDGFRß(+) immunoreactivity mostly in the CA1 region of MAM rats. CONCLUSION: Our descriptive study points to microvascular-pericyte changes in the epileptic pathology. The possible link between PDGFRß(+) cells, neuro-vascular dysplasia and remodeling during seizures is discussed.


Subject(s)
Cerebral Cortex/blood supply , Cerebral Cortex/pathology , Epilepsy, Temporal Lobe/pathology , Malformations of Cortical Development/pathology , Pericytes/pathology , Receptor, Platelet-Derived Growth Factor beta/metabolism , Adolescent , Adult , Animals , Calcium-Binding Proteins , Cerebral Cortex/abnormalities , Cerebral Cortex/metabolism , Child , Child, Preschool , DNA-Binding Proteins/metabolism , Disease Models, Animal , Epilepsy, Temporal Lobe/complications , Epilepsy, Temporal Lobe/metabolism , Epilepsy, Temporal Lobe/physiopathology , Hippocampus/blood supply , Hippocampus/metabolism , Hippocampus/pathology , Hippocampus/physiopathology , Humans , Infant , Malformations of Cortical Development/complications , Malformations of Cortical Development/metabolism , Malformations of Cortical Development/physiopathology , Microfilament Proteins , Pericytes/metabolism , Rats , Rats, Sprague-Dawley , Seizures/complications , Young Adult
2.
Mol Psychiatry ; 18(10): 1096-105, 2013 Oct.
Article in English | MEDLINE | ID: mdl-23032875

ABSTRACT

Cognitive impairments are common in depression and involve dysfunctional serotonin neurotransmission. The 5-HT1B receptor (5-HT(1B)R) regulates serotonin transmission, via presynaptic receptors, but can also affect transmitter release at heterosynaptic sites. This study aimed at investigating the roles of the 5-HT(1B)R, and its adapter protein p11, in emotional memory and object recognition memory processes by the use of p11 knockout (p11KO) mice, a genetic model for aspects of depression-related states. 5-HT(1B)R agonist treatment induced an impairing effect on emotional memory in wild type (WT) mice. In comparison, p11KO mice displayed reduced long-term emotional memory performance. Unexpectedly, 5-HT(1B)R agonist stimulation enhanced memory in p11KO mice, and this atypical switch was reversed after hippocampal adeno-associated virus mediated gene transfer of p11. Notably, 5-HT(1B)R stimulation increased glutamatergic neurotransmission in the hippocampus in p11KO mice, but not in WT mice, as measured by both pre- and postsynaptic criteria. Magnetic resonance spectroscopy demonstrated global hippocampal reductions of inhibitory GABA, which may contribute to the memory enhancement and potentiation of pre- and post-synaptic measures of glutamate transmission by a 5-HT(1B)R agonist in p11KO mice. It is concluded that the level of hippocampal p11 determines the directionality of 5-HT(1B)R action on emotional memory processing and modulates hippocampal functionality. These results emphasize the importance of using relevant disease models when evaluating the role of serotonin neurotransmission in cognitive deficits related to psychiatric disorders.


Subject(s)
Annexin A2/physiology , Avoidance Learning/physiology , Emotions/physiology , Hippocampus/physiology , Memory/physiology , Receptor, Serotonin, 5-HT1B/physiology , S100 Proteins/physiology , Animals , Annexin A2/deficiency , Annexin A2/genetics , Avoidance Learning/drug effects , Depression/physiopathology , Disease Models, Animal , Exploratory Behavior/drug effects , Exploratory Behavior/physiology , Female , Genes, Reporter , Glutamic Acid/metabolism , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Nuclear Magnetic Resonance, Biomolecular , Phosphorylation/drug effects , Presynaptic Terminals/metabolism , Protein Processing, Post-Translational/drug effects , Pyridines/pharmacology , Reaction Time , Receptors, AMPA/metabolism , Recombinant Fusion Proteins/metabolism , S100 Proteins/deficiency , S100 Proteins/genetics , Serotonin 5-HT1 Receptor Agonists/pharmacology , Synaptic Transmission/drug effects , Synaptic Transmission/physiology , Transduction, Genetic
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