ABSTRACT
The genotoxic effect of two tanshinones isolated from roots of Hyptis martiussi Benth (Labiatae) was studied using V79 (Chinese hamster lung) cells by the alkaline comet assay and micronucleus test. Tanshinones were incubated with the cells at concentrations of 1, 3, 6 and 12 microg/mL for 3 h. Tanshinones were shown to be quite strongly genotoxic against V79 cells at all tested concentrations. The data obtained provide support to the view that tanshinones has DNA damaging activity in cultured V79 cells under the conditions of the assays.
Subject(s)
Antioxidants/therapeutic use , Carbon Tetrachloride Poisoning/prevention & control , Chemical and Drug Induced Liver Injury/prevention & control , Flavonoids/therapeutic use , Animals , Blood Chemical Analysis , Carbon Tetrachloride Poisoning/pathology , Catalase/metabolism , Chemical and Drug Induced Liver Injury/pathology , Fatty Liver/chemically induced , Fatty Liver/pathology , Glutathione/metabolism , Glutathione Transferase/metabolism , Hepatocytes/drug effects , Hepatocytes/pathology , Lipid Peroxidation/drug effects , Liver/pathology , Male , Malondialdehyde/metabolism , Oxidative Stress/drug effects , Plant Extracts , Rats , Rats, Sprague-Dawley , Superoxide Dismutase/metabolism , Thiobarbituric Acid Reactive Substances/metabolismABSTRACT
Two abietane diterpenes were isolated from a hexane extract of Hyptis martiusii roots and identified as carnasol 11,14-dihidroxy-8,11,13-abietatrien-7-one. These compounds were tested for their antiproliferative effects on tumor cell lines using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide and on the sea urchin egg development. Both compounds displayed cytotoxic activity against tumor cell lines, but only carnasol was able to inhibit the sea urchin egg cleavages.
