ABSTRACT
Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected cells, the ability of both vectors to induce a specific gD immune response was tested in BALB/c mice that were intramuscularly immunized. Specific serum antibody responses were detected in mice inoculated with both vectors, and the response against truncated gD was higher than the response against full-length gD. These results reinforce previous findings that HSV-1 amplicon vectors can potentially deliver antigens to animals and highlight the prospective use of these vectors for treating infectious bovine rhinotracheitis disease.
Subject(s)
Genetic Vectors/immunology , Herpesvirus 1, Bovine/immunology , Herpesvirus 1, Human/immunology , Infectious Bovine Rhinotracheitis/immunology , Viral Proteins/immunology , Animals , Antibodies, Viral/blood , Blotting, Western/veterinary , Cattle , Female , Herpesvirus 1, Bovine/genetics , Herpesvirus 1, Human/genetics , Immunity, Humoral/immunology , Immunization/methods , Immunization/veterinary , Infectious Bovine Rhinotracheitis/prevention & control , Infectious Bovine Rhinotracheitis/virology , Mice , Mice, Inbred BALB C , Neutralization Tests/veterinary , Specific Pathogen-Free Organisms , Viral Proteins/genetics , Viral Vaccines/immunologyABSTRACT
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Subject(s)
Humans , Male , Female , Infant , Child, Preschool , In Vitro Techniques , Respiratory Tract Infections , Streptococcus pneumoniae/isolation & purification , Haemophilus influenzae/isolation & purification , Staphylococcus aureus/isolation & purification , Respiratory Tract Infections/diagnosis , Respiratory Tract Infections/etiology , Respiratory Tract Infections/microbiology , Bronchiolitis, Viral/microbiology , Bronchiolitis, Viral/diagnosis , Lung Diseases/microbiology , Lung Diseases/diagnosisABSTRACT
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