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4.
Biull Eksp Biol Med ; 89(5): 601-3, 1980 May.
Article in Russian | MEDLINE | ID: mdl-6772256

ABSTRACT

The factors potentiating the efficacy of fusion of protoplasts of Bacillus subtilis were studied. A 50-fold increase in the yield of primary prototrophs was provided by 90-minute preincubation of protoplasts in a liquid hyperbaric medium and 10-minute treatment with trypsin. Increased efficacy of fusion of protoplasts depended on their regeneration percentage.


Subject(s)
Bacillus subtilis/genetics , Protoplasts/physiology , Amino Acids/pharmacology , Bacillus subtilis/drug effects , Hybridization, Genetic , Hypertonic Solutions , Protoplasts/drug effects , Trypsin/pharmacology
5.
Vestn Khir Im I I Grek ; 122(5): 11-5, 1979 May.
Article in Russian | MEDLINE | ID: mdl-452260

ABSTRACT

The experience with the surgical treatment of 97 patients subjected to late repeated operations on the lungs and pleura is generalized in this work. 68% of these patients had pleural empyema and bronchial fistulae. The authors prefer the radical surgery to pulmonectomy, pleuropulmonectomy, decortication, lung resection and repeated amputation of the main bronchus. Fatality in the late repeated operations was noted in 3.09% of cases and complications--in 10.3%.


Subject(s)
Bronchial Fistula/surgery , Bronchiectasis/surgery , Empyema/surgery , Pleura/surgery , Pneumonectomy/methods , Postoperative Complications/surgery , Aged , Drainage/methods , Evaluation Studies as Topic , Humans , Middle Aged , Thoracoplasty
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