ABSTRACT
Serological follow-ups were performed in 19 newborns carrying HIV 1 antibodies over a period ranging between 9 and 62 months. Eight children developed AIDS ans 11 remained asymptomatic. Anti-HIV 1 antibodies were determined by ELISA and Western blot and p 24 antigen was detected by ELISA. Antibodies to HIV 1 disappeared in most children without AIDS symptoms between 10 and 12 months after birth, with antibodies against gp 41 being lost first. Children with AIDS remained positive during follow-up, although in 3 cases with positive was absent in all the asymptomatic children, while it was present in 75% of the patients with AIDS.
Subject(s)
Acquired Immunodeficiency Syndrome/diagnosis , HIV Seropositivity/diagnosis , Acquired Immunodeficiency Syndrome/enzymology , Acquired Immunodeficiency Syndrome/epidemiology , Acquired Immunodeficiency Syndrome/immunology , Enzyme-Linked Immunosorbent Assay , Follow-Up Studies , HIV Antibodies/immunology , HIV Seropositivity/enzymology , HIV Seropositivity/immunology , Humans , Infant, Newborn , Spain/epidemiologyABSTRACT
Sera from 38 tuberculous patients and 62 healthy controls (31 PPD skin test positive and 31 negative) were assayed, by enzyme-linked immunosorbent assay (ELISA), to test the activity of IgG and IgM antibodies against purified protein derivative (PPD) antigen and a phenolglycolipid antigen (PLG-Tb 1) isolated and purified from Mycobacterium tuberculosis strain Canetti. Using PPD antigen, the sensitivity and specificity were respectively, 50 and 93.5% for IgG and 71.1 and 59.7% for IgM antibody activity. Against PGL-Tb 1 antigen, IgG had a sensitivity of 94.7% and the specificity was 96.8%, for IgM antibody they were 65.8% and 75.8% respectively. The ELISA using PGL-Tb 1 antigen could be a useful way to develop a rapid technique to aid in the diagnosis of tuberculosis.
Subject(s)
Antigens, Bacterial , Glycolipids/immunology , Mycobacterium tuberculosis/immunology , Tuberculosis/diagnosis , Antibodies, Bacterial/isolation & purification , Diagnostic Errors , Enzyme-Linked Immunosorbent Assay , Evaluation Studies as Topic , Humans , Immunoglobulin G/isolation & purification , Immunoglobulin M/isolation & purification , Serologic Tests , Tuberculin , Tuberculosis/immunology , Tuberculosis/microbiologySubject(s)
Cytomegalovirus Infections/microbiology , Cytomegalovirus/isolation & purification , Adolescent , Adult , Cells, Cultured , Child , Child, Preschool , Embryo, Mammalian , Female , Humans , Infant , Infant, Newborn , Male , Middle Aged , Postoperative Complications/microbiology , Pregnancy , Pregnancy Complications, Infectious/microbiology , Virus CultivationABSTRACT
Two patients who had a huge pericardial effusion of at least 9 and 14 years' duration caused by cardiac toxoplasmosis are reported. Toxoplasma gondii were seen in the pericardial fluid, and serologic evidence also demonstrated the activity of the infection. These cases illustrate both the need to exclude toxoplasmosis in chronic pericardial effusion of unknown cause and the possibility of seeing toxoplasma in the pericardial fluid of patients with active toxoplasmic pericarditis. Moreover, in endemic areas, cardiac toxoplasmosis may not be an exceptional cause of chronic pericardial effusion.
