ABSTRACT
This article is about how to improve the positional dispensing accuracy of low-volume (microliters) drops and to understand the factors affecting them. These same parameters can be investigated to reduce deleterious effects on dispensing performance. Many applications, such as those in immunoassay diagnostics, require accurate volumetric dispensing and are also less tolerant of other phenomena that cause the reagent to be located outside the target area of interest. In this article, we work with liquid inertia. This phenomenon is present in positional dispensing and affects its accuracy. The negative effect of liquid inertia is reduced by changing parameters such as pressure and the diameter of the involved conducts.
Subject(s)
Immunologic Tests/instrumentation , Microfluidic Analytical Techniques/instrumentation , Microfluidic Analytical Techniques/statistics & numerical data , Animals , Humans , Microfluidic Analytical Techniques/standards , Reproducibility of ResultsABSTRACT
En el desarrollo de los pesquisajes, especialmente en el caso de los neonatales, es muy común que las muestras se colecten en forma de sangre seca sobre papel de filtro, lo que hace imprescindible el empleo de una placa para eluir las muestras. Este trabajo describe el desarrollo y construcción de una placa de 96 pocillos para el empleo en la elución y transferencia automatizadas de muestras, en ensayos para pesquisajes neonatales que utilizan la técnica de papel de filtro. La placa se obtiene a través de la tecnología del termoconformado de láminas de PVC(AU)
