Do we know the cellular location of heavy metals in seaweed? An up-to-date review of the techniques.

Seaweeds are dominant organisms in coastal environments. However, in the context of global change, the integrity of these organisms is threatened by metal pollution. It is therefore important to understand how seaweeds are affected by metal concentrations in the water. Measuring the concentrations of metals in seaweed provides information about the effects of metal pollution on the seaweeds themselves and their ecosystems. Nonetheless, correct interpretation of this type of analysis requires knowledge of the cellular location of the pollutants, as the effects will differ depending on whether the metals are present in particles adhered to the surface, attached to external polysaccharides or dissolved in the cytoplasm. Thus, the objectives of this study were to compile the available information on the subcellular distribution of metals in seaweeds and to conduct a critical review of the information. We found that the existing studies provide contrasting, sometimes contradictory, results. Thus, metals have been detected entirely intracellularly and also mainly outside of the cells. In all of the studies reviewed, which used different techniques (mainly extracellular elution, X-ray microanalysis and centrifugation), methodological and/or conceptual problems were identified that raise questions about the effectiveness of each approach. To obtain reliable information about the distribution of metals in algal cells, further studies must be conducted that take into consideration the differences between elements and algal species and the limits of the methods used to measure the elements.

Sequestration and efflux largely account for cadmium and copper resistance in the deep-sea Nitratiruptor sp. SB155-2 (phylum Campylobacterota).

In deep-sea hydrothermal vent environments, metal-enriched fluids and sediments abound, making these habitats ideal to study metal resistance in prokaryotes. In this investigation, we employed transcriptomics and shotgun proteomics with scanning transmission electron microscopy and energy-dispersive x-ray spectroscopy (STEM-EDX) to better understand mechanisms of tolerance for cadmium (Cd) and copper (Cu) at stress-inducing concentrations in Nitratiruptor sp. SB155-2 (phylum Campylobacterota). Transcriptomic profiles were remarkably different in the presence of these two metals, displaying 385 (19%) and 629 (31%) differentially transcribed genes (DTG) in the presence of Cd(II) and Cu(II), respectively, while only 7% of differentially transcribed (DT) genes were shared, with genes for non-specific metal transporters and genes involved in oxidative stress-response predominating. Transcriptomic and proteomic analyses confirmed that metal-specific DT pathways under Cu(II) stress, including those involving sulfur, cysteine, and methionine, are likely required for high-affinity efflux systems, while flagella formation and chemotaxis were over-represented under Cd(II) stress. Consistent with these differences, STEM-EDX analysis revealed that polyphosphate-like granules (pPLG), the formation of CdS particles, and the periplasmic space are crucial for Cd(II) sequestration. Overall, this study provides new insights regarding metal-specific adaptations of Campylobacterota to deep-sea hydrothermal vent environments.

Extreme storms cause rapid but short-lived shifts in nearshore subtropical bacterial communities.

Climate change scenarios predict tropical cyclones will increase in both frequency and intensity, which will escalate the amount of terrestrial run-off and mechanical disruption affecting coastal ecosystems. Bacteria are key contributors to ecosystem functioning, but relatively little is known about how they respond to extreme storm events, particularly in nearshore subtropical regions. In this study, we combine field observations and mesocosm experiments to assess bacterial community dynamics and changes in physicochemical properties during early- and late-season tropical cyclones affecting Okinawa, Japan. Storms caused large and fast influxes of freshwater and terrestrial sediment - locally known as red soil pollution - and caused moderate increases of macronutrients, especially SiO2 and PO4 3-, with up to 25 and 0.5 µM respectively. We detected shifts in relative abundances of marine and terrestrially derived bacteria, including putative coral and human pathogens, during storm events. Soil input alone did not substantially affect marine bacterial communities in mesocosms, indicating that other components of run-off or other storm effects likely exert a larger influence on bacterial communities. The storm effects were short-lived and bacterial communities quickly recovered following both storm events. The early- and late-season storms caused different physicochemical and bacterial community changes, demonstrating the context-dependency of extreme storm responses in a subtropical coastal ecosystem.

Differential Metal Tolerance and Accumulation Patterns of Cd, Cu, Pb and Zn in the Liverwort Marchantia polymorpha L.

In this study, we investigated the bioaccumulation, tissue distribution and physiological responses to different metal concentration (0.2 and 2 mM) and time of exposure of 1, 2 and 3 weeks with cadmium (Cd), copper (Cu), lead (Pb) and zinc (Zn) using the model liverwort Marchantia polymorpha. Our data showed, on one hand, a significant enrichment and tissue translocation of Cu, Zn, and specially Cd, reaching concentrations of 1800 µg g- 1 in 3 weeks. On the other hand, Pb exhibited the lowest concentration values (50 µg g- 1), and 90% of the total concentration in the rhizoids. We could observe a positive correlation between tissue concentration, metal translocation and an enhanced toxic response. The results obtained in this study might contribute not only in the application of this species in environmental studies with heavy metals but also as a starting point to study the evolution of metal tolerance in land plants.

Study of the air quality in industrial areas of Santa Cruz de Tenerife (Spain) by active biomonitoring with Pseudoscleropodium purum.

A biomonitoring technique with terrestrial moss transplants (50 sampling sites in a regular grid) was used in an area of the city of Santa Cruz de Tenerife, close to an oil refinery and to an area of dense road traffic for a period of 2 months. The concentration of metals and metalloids (As, Cd, Hg, Ni, Pb and V) and 16 polycyclic aromatic hydrocarbons (PAHs) were determined. The density distribution was represented, the enrichment factors calculated and multifactorial analysis applied. In addition, contamination maps were elaborated on the basis of the bioconcentration obtained, and after confirming the existence of spatial structure, the response surfaces were represented. The results showed very high levels of contamination by Ni and V in the study area, with similar dispersal patterns observed for both. The concentrations of Cd, Hg, Pb and PAHs were lower. Active biomonitoring with terrestrial mosses was found to be a suitable technique for implementing inexpensive environmental monitoring programmes in urban and industrialized areas.