Electroanalysis from the past to the twenty-first century: challenges and perspectives.

A personal mini-review is presented on the history of electroanalysis and on their present achievements and future challenges. The manuscript is written from the subjective view of two generations of electroanalytical chemists that have witnessed for many years the evolution of this discipline.

Screen-Printed Electrodes for the Voltammetric Sensing of Benzotriazoles in Water.

Benzotriazoles (BZTs) are high production volume industrial chemicals that are used in various applications such as corrosion inhibitors, antifreeze agents, and UV radiation stabilizers. Given their potential ecotoxicological implications for different ecosystems and in human health, as well as their poor biodegradability, they are of increasing concern. In this study, a new voltammetric method using commercial screen-printed electrodes (SPEs) has been developed for the sensing of BZTs in water samples to help in their environmental monitoring. To this end, different types of SPEs based on carbon nanoallotropes and copper were tested under several experimental conditions to determine the two BZTs most frequently detected in the environment: 1H-benzotriazole (BZT) and 5-methyl-1H-benzotriazole (Me-BZT, tolyltriazole) as model compounds for BZTs. Carbon nanofibers electrodes exhibited the best performance, allowing detection limits as low as 0.4 mg L-1 for both BZTs, with repeatability and reproducibility of ca. 5%. The applicability of the method was tested through the determination of BZT in spiked drinking water samples, suggesting its suitability for the sensing of samples heavily polluted with BZTs.

New Approach to Multivariate Standard Addition Based on Multivariate Curve Resolution by Alternating Least-Squares: Application to Voltammetric Data.

A multivariate version of the classical univariate standard addition method is proposed for the analysis of samples generating overlapping signals in the presence of notorious matrix effects. Unlike previous versions based on multivariate calibration by partial least-squares (PLS), the proposed strategy takes advantage of a self-modeling methodology: multivariate curve resolution by alternating least-squares (MCR-ALS) enhanced with signal shape constraints based on parametric functions. In this way, there is no need for the full multivariate response of a blank solution, and in multianalyte determinations, the standard additions can be made with a solution containing all of the analytes, which constitutes a clear advance as compared to PLS approach. The proposed method has been successfully tested in the voltammetric determination of hydroquinone and catechol in solutions of increasing complexity and appears to be a promising tool in the field of electroanalysis.

Voltammetric Electronic Tongues in Food Analysis.

A critical revision is made on recent applications of voltammetric electronic tongues in the field of food analysis. Relevant works are discussed dealing with the discrimination of food samples of different type, origin, age and quality and with the prediction of the concentration of key substances and significant indexes related to food quality.

Commercial Screen-Printed Electrodes Based on Carbon Nanomaterials for a Fast and Cost-Effective Voltammetric Determination of Paracetamol, Ibuprofen and Caffeine in Water Samples.

Carbon screen-printed electrode (SPCE), multi-walled carbon nanotubes modified screen-printed electrode (SPCNTE), carbon nanofibers modified screen-printed electrode (SPCNFE), and graphene modified screen-printed electrode (SPGPHE) were in a pioneer way tested as sensors for the simultaneous determination of the two most consumed pain-killers, paracetamol (PA) and ibuprofen (IB), and the stimulant caffeine (CF) in water by differential pulse voltammetry (DPV). Their analytical performances were compared, and the resulting sensitivities (2.50, 0.074, and 0.24 µA V mg-1 L for PA, IB, and CF, respectively), detection limits (0.03, 0.6, and 0.05 mg L-1 for PA, IB, and CF, respectively) and quantification limits (0.09, 2.2, and 0.2 mg L-1 for PA, IB, and CF, respectively) suggested that the SPCNFE was the most suitable carbon-based electrode for the voltammetric determination of the selected analytes in water at trace levels. The methodology was validated using both spiked tap water and hospital wastewater samples. The results were compared to those achieved by liquid chromatography-tandem mass spectrometry (LC-MS/MS), the technique of choice for the determination of the target analytes.

A new multivariate standard addition strategy for stripping voltammetric electronic tongues: Application to the determination of Tl(I) and In(III) in samples with complex matrices.

A new multivariate standard addition strategy applicable to stripping methods was proposed as an extention of the classical univariate standard addition method for the resolution of complex samples involving overlapped peaks and complex matrices. The proposed strategy consists in alternate additions of the considered analytes and the further extrapolation to a simulated blank solution measured by skipping the preconcentration step (deposition time = 0). This calibration approach was successfully tested in tonic water samples spiked with Tl(I) and In(III) using a sensor array based on a SeCyst-SPCNFE and an ex-situ-BiSPCE, providing good concordance between replicates and much better accuracy than the usual multivariate external calibration method.

Characterization and classification of Spanish paprika (Capsicum annuum L.) by liquid chromatography coupled to electrochemical detection with screen-printed carbon-based nanomaterials electrodes.

Screen-printed electrodes based on graphite, carbon nanotubes, carbon nanofibers, and graphene were tested as amperometric detectors for the determination of phenolic compounds by high performance liquid chromatography (HPLC). The chromatographic performance as well as the obtained sensitivity, detection and quantification limits suggest that carbon nanofibers modified screen-printed electrode (SPCE-CNF) is the amperometric sensor that provides the best analytical performance. Upon this confirmation, chromatographic data obtained using SPCE-CNF were exploited by means of linear discriminant analysis (LDA) to successfully characterize and classify 96 Spanish paprika (Capsicum annuum L.) samples with different origin and type: from La Vera (including sweet, bittersweet and spicy types) and from Murcia (including sweet and spicy types).

Voltammetric determination of metal ions beyond mercury electrodes. A review.

For a long time mercury electrodes have been the main choice for the analysis of metal ions and some metalloids. However, in the last years, safety and environmental considerations have restricted their use and encouraged the search for alternative materials more environmentally friendly and with more possibilities for in-situ and flow analysis. This research has been reinforced by the popularisation of nanomaterials, biomolecules and screen-printed electrodes, as well as for the new advances in sensor miniaturization and integration of the electrodes in multi-sensor platforms and electronic tongues. The present review critically summarizes and discusses the progress made since 2010 in the development and application of new electrodes for the analysis of metals and metalloids.

Selenocystine modified screen-printed electrode as an alternative sensor for the voltammetric determination of metal ions.

A novel selenium based screen-printed electrode was developed based on the immobilization of selenocystine on aryl diazonium salt monolayers anchored to a carbon-nanofiber screen-printed electrode support (SeCyst-SPCNFE). SeCyst-SPCNFE was analytically compared to a screen-printed carbon nanofiber electrode modified with L-Cystine (Cyst-SPCNFE) for the determination of Pb(II) and Cd(II) by stripping voltammetric techniques. Their analytical performance suggests that SeCyst-SPCNFE could be a much better alternative for metal ion determination at trace levels than Cyst-SPCNFE. The proposed electrode was successfully applied for the simultaneous voltammetric determination of trace Pb(II) and Cd(II) in a wastewater reference material with a very high reproducibility (3.2%) and good trueness (2.6%).

Ag Nanoparticles Drop-Casting Modification of Screen-Printed Electrodes for the Simultaneous Voltammetric Determination of Cu(II) and Pb(II).

A new silver nanoparticle modified screen-printed electrode was developed and applied to the simultaneous determination of Pb(II) and Cu(II). Two different types of silver nanoparticles with different shapes and sizes, Ag nanoseeds and Ag nanoprisms, were microscopically characterized and three different carbon substrates, graphite, graphene and carbon nanofibers, were tested. The best analytical performance was achieved for the combination of Ag nanoseeds with a carbon nanofiber modified screen-printed electrode. The resulting sensor allowed the simultaneous determination of Pb(II) and Cu(II) at trace levels and its applicability to natural samples was successfully tested with a groundwater certified reference material, presenting high reproducibility and trueness.

Phytochelatin synthesis in response to Hg uptake in aquatic plants near a chlor-alkali factory.

The effects of mercury (Hg) released from a chlor-alkali factory in aquatic plants along the Ebro River basin (NE Spain) were analysed considering the phytochelatins (PCn) and their isoforms content in these plants. These compounds were analyzed using HPLC with amperometric detection, and the macrophytes species Ceratophyllum demersum and Myriopyllum spicatum were collected in two sampling campaigns, autumn and spring, respectively. To correlate the PCn content in macrophytes with the Hg contamination, analysis of total Hg (THg) content in plants and suspended particulate matter, as well as the dissolved-bioavailable fraction of Hg in water measured by the diffusive gradient in thin film (DGT) technique were done. The results confirm the presence of PC2-Ala in extracts of C. demersum and PC2-desGly in M. spicatum, and the concentration of these thiol compounds depends clearly on the distance between the hot spot and the downstream sites: the higher the levels are, the closer the hot spot is. Since most of the Hg is hypothesized to be associated with SPM and transported downstream, our results of the DGT suggest that trace amounts of Hg in water can be released as free metal ions yielding a certain accumulation in plants (reaching the ppb level) that are enough for activation of induction of PCs. A few PCs species have been determined, at different seasons, indicating that they can be used as good indicators of the presence of bioavailable Hg in aquatic media throughout the year.

Determination of Pd(II) using an antimony film coated on a screen-printed electrode by adsorptive stripping voltammetry.

The use of an antimony film coated on a screen-printed carbon electrode (ex-situ SbSPCE) is proposed for the determination of Pd(II) at ppb levels in natural samples by adsorptive stripping voltammetry using dimethylglyoxime as chelating agent. Ex-situ SbSPCE produces a better analytical performance as compared to a commercially sputtered bismuth screen-printed electrode (BispSPE). The detection and quantification limits were 2.7 and 9.0µgL-1 respectively with a good linear behaviour in the wide examined concentration range (from 1µgL-1 up to 100.0µgL-1, R2=0.998). The proposed ex-situ SbSPCE showed an excellent repeatability with a relative standard deviation of 0.5% for ten successive measurements and a very good reproducibility (1.6% for three different ex-situ SbSPCE units within series of ten repetitive assays). Moreover, the ex-situ SbSPCE was successfully applied for the determination of low concentration levels of Pd(II) in spiked tap water with a very high reproducibility (0.2%) and providing equivalent results to those achieved by ICP-MS measurements.

Simultaneous determination of hydroquinone, catechol and resorcinol by voltammetry using graphene screen-printed electrodes and partial least squares calibration.

Catechol (CC), resorcinol (RC) and hydroquinone (HQ) are dihydroxybenzene isomers that usually coexist in different samples and can be determined using voltammetric techniques taking profit of their fast response, high sensitivity and selectivity, cheap instrumentation, simple and timesaving operation modes. However, a strong overlapping of CC and HQ signals is observed hindering their accurate analysis. In the present work, the combination of differential pulse voltammetry with graphene screen-printed electrodes (allowing detection limits of 2.7, 1.7 and 2.4µmolL(-1) for HQ, CC and RC respectively) and the data analysis by partial least squares calibration (giving root mean square errors of prediction, RMSEP values, of 2.6, 4.1 and 2.3 for HQ, CC and RC respectively) has been proposed as a powerful tool for the quantification of mixtures of these dihydroxybenzene isomers. The commercial availability of the screen-printed devices and the low cost and simplicity of the analysis suggest that the proposed method can be a valuable alternative to chromatographic and electrophoretic methods for the considered species. The method has been applied to the analysis of these isomers in spiked tap water.

Determination of Sb(III) using an ex-situ bismuth screen-printed carbon electrode by adsorptive stripping voltammetry.

The determination of Sb(III) on an ex-situ bismuth screen-printed carbon electrode (ex-situ BiSPCE) by means of adsorptive stripping voltammetry (AdSV) using quercetin-5'-sulfonic acid as chelating agent was optimized. The effect of different experimental parameters such pH, ligand concentration (CQSA), accumulation potential (Eacc) and accumulation time (tacc) were studied to obtain a wide linear range, the highest sensitivity and the lowest detection limit. Ex-situ BiSPCE was analytically compared with a sputtered bismuth screen-printed electrode (BispSPE) under optimal conditions. The obtained analytical parameters suggest that ex-situ BiSPCE behaves much better than BispSPE and the first was selected for this study. Optimal parameters were pH=4.6; CQSA=10.0 to 20.0×10(-6)molL(-1); Eacc=-0.5V and tacc=60s. Peak area is proportional to Sb(III) concentration up to 100.0µgL(-1) (tacc 60s) and 45.0µgL(-1) (tacc 120s) range, with detection limits of 1.2µgL(-)(1) (tacc 60s) and 0.8µgL(-1) (tacc 120s). The relative standard deviation for a Sb(III) solution (20.0µgL(-1)) was 3.9% for ten successive assays. Thus, the effect of various interfering metal ions was studied and the methodology was validated using a spiked groundwater reference material with very satisfactory results.

Glutathione modified screen-printed carbon nanofiber electrode for the voltammetric determination of metal ions in natural samples.

This work reports the development of a glutathione modified electrode via electrografting on a screen-printed carbon nanofiber substrate (GSH-SPCNFE). GSH-SPCNFE was compared to a classical screen-printed carbon electrode modified with glutathione (GSH-SPCE) for the simultaneous voltammetric determination of Cd(II) and Pb(II). Their electrochemical characterization and analytical performance suggest that SPCNFE could be a much better support for GSH immobilization. The applicability of GSH-SPCNFE for the determination of low concentration levels of Pb(II) and Cd(II) ions in environmental samples was successfully tested in a certified wastewater reference material by means of stripping voltammetry with a very high reproducibility and good trueness.

New approaches to antimony film screen-printed electrodes using carbon-based nanomaterials substrates.

Three different commercial carbon nanomaterial-modified screen-printed electrodes based on graphene, carbon nanotubes and carbon nanofibers were pioneeringly tested as electrode platforms for the plating with Sb film. They were microscopically and analytically compared to each other and to the most conventional unmodified carbon screen-printed electrode (SPCE). The obtained detection and quantification limits suggest that the in-situ antimony film electrode prepared from carbon nanofibers modified screen-printed electrode (SbSPCE-CNF) produces a better analytical performance as compared to the classical SPCE modified with antimony for Pb(II) and Cd(II) determination, approving its appropriateness for measuring low µg L(-1) levels of the considered metals. In-situ SbSPCE-CNF was successfully used for the simultaneous determination of Pb(II) and Cd(II) ions, by means of differential pulse anodic stripping voltammetry, in a certified reference estuarine water sample with a very high reproducibility and good trueness.

Penicillamine-modified sensor for the voltammetric determination of Cd(II) and Pb(II) ions in natural samples.

A new penicillamine-GCE was developed based on the immobilization of d-penicillamine on aryl diazonium salt monolayers anchored to the glassy carbon electrode (GCE) surface and it was applied for the first time to the simultaneous determination of Cd(II) and Pb(II) ions by stripping voltammetric techniques. The detection and quantification limits at levels of µg L(-1) suggest that the penicillamine-GCE could be fully suitable for the determination of the considered ions in natural samples.

Carbon nanotubes and graphene modified screen-printed carbon electrodes as sensitive sensors for the determination of phytochelatins in plants using liquid chromatography with amperometric detection.

Nanomaterials are of great interest for the development of electrochemical sensors. Multi-walled carbon nanotubes and graphene were used to modify the working electrode surface of different screen-printed carbon electrodes (SPCE) with the aim of improving the sensitivity of the SPCE and comparing it with the conventional glassy carbon electrode. To assay the usability of these sensors, a HPLC methodology with amperometric detection was developed to analyze several phytochelatins in plants of Hordeum vulgare and Glycine max treated with Hg(II) or Cd(II) giving detection limits in the low µmolL(-1) range. Phytochelatins are low molecular weight peptides with the general structure γ-(Glu-Cys)n-Gly (n=2-5) which are synthesized in plants in the presence of heavy metal ions. These compounds can chelate heavy metal ions by the formation of complexes which, are transported to the vacuoles, where the toxicity is not threatening. For this reason phytochelatins are essential in the detoxification of heavy metal ions in plants. The developed HPLC method uses a mobile phase of 1% of formic acid in water with KNO3 or NaCl (pH=2.00) and 1% of formic acid in acetonitrile. Electrochemical detection at different carbon-based electrodes was used. Among the sensors tested, the conventional glassy carbon electrode offers the best sensitivity although modification improves the sensitivity of the SPCE. Glutathione and several isoforms of phytochelatin two were found in plant extracts of both studied species.

Antimony film screen-printed carbon electrode for stripping analysis of Cd(II), Pb(II), and Cu(II) in natural samples.

An in-situ antimony film screen-printed carbon electrode (in-situ SbSPCE) was successfully used for the determination of Cu(II) simultaneously with Cd(II) and Pb(II) ions, by means of differential pulse anodic stripping voltammetry (DPASV), in a certified reference groundwater sample with a very high reproducibility and good trueness. This electrode is proposed as a valuable alternative to in-situ bismuth film electrodes, since no competition between the electrodeposited copper and antimony for surface sites was noticed. In-situ SbSPCE was microscopically characterized and experimental parameters such as deposition potential, accumulation time and pH were optimized. The best voltammetric response for the simultaneous determination of Cd(II), Pb(II) and Cu(II) ions was achieved when deposition potential was -1.2 V, accumulation time 120 s and pH 4.5. The detection and quantification limits at levels of µg L(-1) suggest that the in-situ SbSPCE could be fully suitable for the determination of Cd(II), Pb(II) and Cu(II) ions in natural samples.

Evaluation of mercury stress in plants from the Almadén mining district by analysis of phytochelatins and their Hg complexes.

To evaluate plant response to Hg stress, glutathione, phytochelatins, and their Hg complexes were analyzed using HPLC with amperometric detection in samples of Asparagus acutifolius grown in the Almadén mining district (Ciudad Real, Spain), one of the most Hg-contaminated sites in the world. Soils of the Almadén mining district, and specifically from the Almadenejos zone, are highly contaminated, with some zones having values above 4,000 µg Hg g(-1) soil. Although soils have an extremely high concentration of mercury, generally less than 2% is available for plants, as is shown by various soil extractions simulating bioavailability. In plants, Hg concentration increases depending on the content of Hg in soils. In addition, Hg levels in roots are higher than in aerial parts, which is a strategy of plants for protecting their more sensitive aerial parts from the deleterious effects of metal stress. The total content of phytochelatins (PCs) and their complexes are directly related with the amount of mercury in soils. These findings highlight the important role of thiol compounds and their metal complexes in capturing and fixing Hg from soils, giving plants the capacity to deal with the heavy metal toxicity of polluted soils.