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Biosci Biotechnol Biochem ; 77(6): 1310-2, 2013.
Article in English | MEDLINE | ID: mdl-23748766

ABSTRACT

Rabbit polyclonal antibody produced by a major royal jelly protein 1 (MRJP1) specific peptide reacted only with a MRJP1. Indirect ELISA with the antibody revealed a MRJP1 level of 4.12-4.67 g/100 g in different company's royal jelly, which almost agreed with that of a hexametric form of MRJP1 (apisin) measured by high performance liquid chromatography. These results suggest that MRJP1 exists mainly as apisin in royal jelly.


Subject(s)
Antibodies/immunology , Fatty Acids/chemistry , Glycoproteins/isolation & purification , Insect Proteins/isolation & purification , Animals , Annexin A2/chemistry , Chromatography, High Pressure Liquid , Enzyme-Linked Immunosorbent Assay , Fatty Acids/metabolism , Glycoproteins/chemistry , Glycoproteins/immunology , Insect Proteins/chemistry , Insect Proteins/immunology
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