ABSTRACT
We studied new immunological and physicochemical properties of K-activin, immunotropic preparation from porcine skin isolated by the acetone method. The preparation restored the sensitivity of background rosette-forming cells in the spleen of thymectomized mice to the inhibitory effect of azathioprine in vivo and practically normalized serum thymic activity reduced in thymectomized mice. The molecular weight of proteins present in K-activin and previously detected by SDS-PAAG electrophoresis was determined by MALDI mass spectrometry
Subject(s)
Dermatologic Agents/pharmacology , Skin , Spleen/drug effects , Adjuvants, Immunologic/chemistry , Adjuvants, Immunologic/pharmacology , Animals , Azathioprine/metabolism , Dermatologic Agents/chemistry , Dermatologic Agents/immunology , Humans , Immunosuppressive Agents/metabolism , Male , Mice , Mice, Inbred C57BL , Skin/chemistry , Skin/immunology , Spleen/cytology , SwineABSTRACT
We studied pharmacological properties of immunotropic preparation from porcine skin (skin resorption effect and acute and chronic toxicity). It was demonstrated that the preparation did not damage the skin during evaluation of skin-resorption effect; evaluation of acute and chronic toxicity revealed no changes in the general state of experimental animals and histological structure of visceral organs and skin; evaluation of chronic toxicity showed that the preparation induced no changes in blood biochemical parameters compared to the control.
Subject(s)
Dermatologic Agents/toxicity , Skin , Activins , Adjuvants, Immunologic/pharmacology , Adjuvants, Immunologic/toxicity , Animals , Dermatologic Agents/pharmacology , Guinea Pigs , Humans , Mice , Ointments/pharmacology , Ointments/toxicity , Rats , Skin/chemistry , Skin/drug effects , Skin/immunology , SwineABSTRACT
The level of natural apoptosis in rat thymus on day 18 of embryo development attained 25%, while at subsequent terms it was about 5%. In the spleen, this parameter gradually decreased from 15 to 37% starting from day 18 of embryo development to postnatal day 30. Tactivin prevented the development of dexamethasone-induced apoptosis in thymocytes of 30-day-old rats, but had no effect on spontaneous apoptosis. Tactivin can be used as a modulator of apoptotic processes.
Subject(s)
Adjuvants, Immunologic/metabolism , Apoptosis/drug effects , Dexamethasone/toxicity , Peptides/metabolism , Thymus Extracts/metabolism , Thymus Gland , Animals , Cells, Cultured , Rats , Spleen/cytology , Spleen/drug effects , Thymus Gland/cytology , Thymus Gland/drug effectsABSTRACT
We studied the effects of ribonucleic immunotropic preparation Ribotim obtained from the thymus on functional activity of peripheral blood neutrophils and wound healing after burn trauma. Immunotherapy normalized immunological parameters, attenuated demarcation inflammation in burn wound, and promoted epithelialization of the wound surface preserving skin structures.
Subject(s)
Adjuvants, Immunologic/pharmacology , Burns/physiopathology , Neutrophils/drug effects , RNA/pharmacology , Tissue Extracts/pharmacology , Wound Healing/drug effects , Adjuvants, Immunologic/therapeutic use , Animals , Burns/drug therapy , Male , Neutrophils/cytology , RNA/therapeutic use , Rats , Rats, Wistar , Thymus Gland/chemistry , Tissue Extracts/therapeutic useABSTRACT
We proposed a method for isolation of low-molecular-weight RNA from mammalian thymus. Physicochemical properties and regulatory activity of these RNA in immunological test of T cell-dependent antibody formation were evaluated.
