An accessory muscle of flexor digitorum profundus with bipennate first lumbrical:a unique variation of clinical significance / 대한해부학회지

During the deep dissection of the front of the forearm, an anomalous accessory muscle in relation to the flexor digitorum profundus (FDP) muscle was observed in the right forearm. The accessory muscle consisted of a spindle-shaped muscle belly with a long tendon underneath the flexor pollicis longus muscle. When followed distally, the accessory muscle tendon was found lateral to the FDP tendon for the index finger and entered the palm deep to the flexor retinaculum. In the palm, we encountered the first lumbrical muscle as a bipennate muscle taking origin from the adjacent sides of the middle of the tendons of FDP and accessory muscle tendon. After giving origin to first lumbrical muscle, the accessory muscle got merged with the tendon of FDP for index finger. Understanding this kind of variation is required for radiologists and hand surgeons for diagnostic purposes and while performing corrective surgical procedures.

Blood Culture Contamination Rate as a Quality Indicator - a Prospective Observational Study.

Introduction: Blood culture test is the gold standard test to diagnose bloodstream infections, but contamination is the main problem in this valuable test. False positive results in blood cultures are mainly due to contamination that occurs mostly during pre-analytical procedures like sample collection and sometimes during sample processing. Materials and method:Our prospective observational study was undertaken at St. Theresa Hospital, Hyderabad, India, during January 2020-June 2020. Blood cultures received from inpatient departments (IPD) and outpatient departments (OPD) are included. Sample size: The contamination rate was calculated by dividing the total number of contaminated blood cultures by the total number of cultures multiplied by 100. Results:Blood culture contamination rate is 2.4%, which is within the limit as per the standard guideline. Conclusion:Contamination occurred mainly due to improper disinfection of the skin and environmental contamination.

Detection of Metallo Beta-Lactamase (MBL) Producing Pseudomonas aeruginosa in a Tertiary Care Hospital, Ghanpur, Medchal, India.

Introduction:Pseudomonas aeruginosa is a leading cause of nosocomial infections and is especially prevalent among patients with burn wounds, cystic fibrosis, acute leukemia, organ transplants and intravenous-drug addiction. Acquired metallo-â-lactamases (MBLs) are carbapenemases which require zinc in the active site and are predominantly produced by P. aeruginosa. They belong to Ambler's class B and Bush-Jacoby Medeiros Group 3 and hydrolyse virtually all â-lactam agents, including the carbapenems. In India, only blaVIM and NDM-1 have been reported in P. aeruginosa. Metallo beta-lactamases have recently emerged as one of the most worrisome resistance mechanisms owing to their capacity to hydrolyse all beta-lactams, including carbapenems. Aims and objectives: The present study aimed to investigate the prevalence and resistance patterns of MBL-producing P. aeruginosa in a tertiary care hospital of Ghanpur, Medchal, India, and to compare the effectiveness of two different methods of screening and detecting MBL-producing P. aeruginosa in order to formulate a policy of empirical therapy and to take preventive measures in hospital settings. Methodology: In the present study, 60 isolates of Pseudomonas aeruginosa were obtained from various clinical specimens, including pus, urine, burns, wound, sputum, pleural fluid, and CSF, which were taken from inpatients and outpatients admitted to MIMS, Ghanpur, India. The study period was from January 2017 to July 2018. The microbial isolates were studied for the detection of the prevalence of MBL production, including their antibiogram. Results:Of the 60 Pseudomonas aeruginosa isolates, 12 were Imipenem resistant, of which nine were MBL producers. Most isolates (14) were collected in the age group of 21-30 years, followed by that of 31-40 years (13) and 1-10 years (2). Of the total number of samples, 40 strains were isolated in male subjects, with a male-female ratio of 2:1. Total wound swabs accounted for 40% of the studied specimens, followed by ear swabs (20%) and sputum samples (18.3%). Wound swabs also included most Imipenem-resistant isolates (41.6%). Metallo beta-lactamase producers accounted for 75% of all carbapenem-resistant isolates, using the combined disc method and E-test. By comparison, DDST retrieved 41% of Pseudomonas MBL producers. Isolates were 100% sensitive to Polymyxin-B and showed a 44.4% sensitivity to Piperacillin/Tazobactam, followed by 22.2% for Amikacin and Tobramycin and 11.1% for Ciprofloxacin and Gentamicin. Conclusion:The study found a relatively high prevalence of Pseudomonas MBL producers (9/60) with 100% Polymyxin susceptibility. Hence, our results warn against an expected high use of Polymyxins in clinical settings. Additionally, the study supports the use of E-tests, CDST and DDST for the screening of Pseudomonas MBL producers in regions where PCR detection cannot be performed.

Variant pectoralis minor muscle: a case study with clinical relevance / 대한해부학회지

The pectoralis minor (PMn) muscle originates from the third, fourth, and fifth ribs near the costochondral junctionusually and gets inserted on the medial margin and upper surface of the coracoid process of the scapula. To look at the morphological insertion patterns and sites of attachment of the PMn muscle in the donated cadavers. Over all 19 limbs were included in the study (9 right and 10 left). Out of 19 limbs, 10 belonged to female and 9 belonged to male cadavers. The cadavers were meticulously dissected to determine the morphological insertion types and location of the attachment of the muscle. Unusual pattern of insertion was observed in 6 limbs (31.6%) out of total 19 limbs included in the study. The variations we observed does not fall completely in the classification by Le Double, hence variations we observed can be considered as new and rare variant which to our knowledge is not reported in literature. We propose this new variant to be type 4 of Le Double classification. The potential of ectopic PMn tendon should be taken into consideration and tested out, especially in patients with shoulder discomfort and stiffening who have ruled out the more frequent diseases. For proper surgical planning, a preoperative magnetic resonance imaging or USG examination of the shoulder joint is required considering the prevalence of variation in the insertion pattern of PMn muscle. Preoperative identification of any abnormal PMn insertion can help to reduce the risk of iatrogenic tendon injury and post-operative problems.

Student Team Achievement Division as a tool for peer assisted co-operative learning in neuroanatomy / 대한해부학회지

Student Team Achievement Division (STAD) is a co-operative learning approach premised on a group learning activity that emphazises learning as a social exchange of knowledge between students, in which each student is accountable for his or her own learning and is also encouraged to assist others in achieving their goals. It promotes the cognitive, psychomotor, and emotional growth of students involved in the team. By random sequencing, 60 participants were allocated to interventional group (n=30) and control group (n=30). The participants of the interventional group were subjected to STAD strategy and participants of the control group were instructed to do a conventional self learning on the ventricles of brain. The outcomes were statistically analysed. It was found that the performance of the students is far better with STAD approach than conventional self learning. Our study has shown that Students team Achievement Division can be used as an effective tool for Peer assisted Co-operative Learning in Anatomy. Further studies can be done to investigate the contribution of STAD to teaching other disciplines of Anatomy and other basic medical sciences.