ABSTRACT
Alginate and galactomannan-derived oligosaccharides enhanced the production of penicillin G when added to stirred tank reactor cultures of Penicillium chrysogenum. The addition of oligomannuronate and oligoguluronate blocks increased penicillin G yield by 47% and 49%, respectively. The effect of mannan oligosaccharides was found to be more pronounced with 69% higher yield than the control cultures. The maximum increase in the average specific productivity of the oligosaccharide augmented cultures was 55% after addition of mannan oligosaccharides. In addition, a difference was observed in all cases in the accumulation pattern of the intermediate of penicillin biosynthesis, delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine.
Subject(s)
Penicillin G/metabolism , Penicillins/biosynthesis , Penicillium chrysogenum/metabolism , Alginates/pharmacology , Bioreactors , Fermentation , Galactose/analogs & derivatives , Kinetics , Mannans/pharmacology , Oligopeptides/metabolism , Oligosaccharides/pharmacology , Penicillium chrysogenum/drug effects , Penicillium chrysogenum/growth & developmentABSTRACT
Oligosaccharide fragments were prepared by partial acid hydrolysis of sodium alginate and consisted of oligomannuronate (OM) and oligoguluronate (OG) blocks. Effects of the OM and OG blocks on penicillin G production by P. chrysogenum were investigated. The oligosaccharides were found to cause significant increases in penicillin G yields. OM blocks at concentrations 10 to 100 microg/mL were used to further evaluate the effects of the oligosaccharides, and were found to enhance the production of penicillin G in shaken flask cultures of P. chrysogenum P2 (high penicillin producer) and NRRL 1951 (low penicillin producer) at the test concentrations. There was an approximately 50% maximum increase in penicillin G yield from biomass in P. chrysogenum P2 cultures and 150% in P. chrysogenum NRRL 1951 cultures, when compared to control cultures without the oligosaccharides. (c) 1997 John Wiley & Sons, Inc.