1.
N Biotechnol
; 27(6): 844-50, 2010 Dec 31.
Article
in English
| MEDLINE
| ID: mdl-20667519
ABSTRACT
Enzyme preparations of Candida antarctica B lipase (CAL-B) - immobilized on Eupergit C and partially modified Eupergit C supports - were tested for kinetic resolution of (R/S)-propranolol, using vinyl acetate as acyl donor, and toluene as organic solvent. The effects of (R/S)-propranolol concentration, vinyl acetate concentration and biocatalyst loading on the esterification and resolution of propranolol were studied. Additionally, different types of immobilized lipase derivatives were also evaluated in terms of its selectivity on kinetic resolution of (R,S)-propranolol. These derivatives showed different enantiomeric ratios (E), with high enantiomeric ratios (E=57) with CAL-B immobilized on Eupergit C supports.