ABSTRACT
Background: Rotary nickel-titanium files have become the most commonly used engine-powered endodontic instruments due to their improvement in fatigue resistance and flexibility. The autoclaving is the most commonly used method for sterilization. However, the risk of instrument fracture, mainly due to cyclic fatigue during rotation within a curved canal, is still a matter of critical concern. Aims: This study aimed to measure and compares the cyclic fatigue resistance of EdgeFile X7, 2Shape, and F-One files in a single curved simulated canal before and after autoclave sterilization. Materials and Methods: Twenty-four files each of EdgeFile X7, 2Shape, and F-One files were used in this study. They were grouped as group A: EdgeFile X7, group B: 2Shape, and group C: F-One files. Then, each group was subdivided into two, sterilized and nonsterilized, subgroups. The files were tested using a custom-made artificial canal. The number of cycles to fracture (NCF) was calculated. Statistical Analysis Used: One-way analysis of variance, post hoc Tukey's test, and independent t-test were used. Results: In nonsterilized instruments, the NCF of the EdgeFile X7 group was significantly higher than that in the other groups (P < 0.05). In sterilized instruments, there was a nonsignificant difference in NCF between EdgeFile X7 and F-One files (P > 0.05). The NCF of 2Shape was significantly lower than other files (P ≤ 0.05) in sterilized and nonsterilized groups. The autoclaving significantly decreased (P ≤ 0.05) the cyclic fatigue resistance of the tested files. Conclusions: EdgeFile X7 was the most fatigue resistant. Autoclaving reduced the cyclic fatigue resistance of the tested files.
ABSTRACT
Morbid obesity (MO) is associated with an increase in circulating levels of systemic acute phase proteins such as C-reactive protein (CRP). Toll-like receptor is possible candidate for inflammatory responses which is mainly mediated by NFKB1. The aim of this study was to investigate the relationship between NFKB1 and Toll-like receptor (TLR) 2 polymorphisms and the risk of MO in a Turkish population in the context of CRP serum levels which may contribute to susceptibility to the disease. We analysed the distribution of NFKB1-94 ins/del ATTG rs28362491 and TLR2 Arg753Gln rs5743708 polymorphisms using PCR-RFLP method and CRP serum levels using ELISA method in 213 MO and 200 healthy controls. The frequency of the ins/ins genotype and ins allele of rs28362491 was significantly higher in the patients compared to control group (P: 0.0309; P: 0.0421, respectively). Additionally, the frequency of GG genotype and G allele of rs5743708 was found to be statistically higher in the patient group (P: 0.0421; P < 0.0001, respectively). In addition, serum CRP levels (>20 mg/l) in MO patients with ins/ins genotype were significantly higher than in patients with del/ins genotype (P: 0.0309). Serum CRP levels were also higher in MO patients with GG genotype and G allele (P: 0.0001). According to combined analysis, the wild type of rs28362491 and rs5743708 polymorphisms (ins/ins/GG genotype) was also significantly higher in the patient group versus the control group when compared with the combined ins/ins/GA and del/ins/GA genotype (P < 0.0001). Therefore, our findings suggest that rs28362491 and rs5743708 polymorphisms were significantly associated with MO disease through acting by modulating serum CRP levels.
Subject(s)
C-Reactive Protein/genetics , Genetic Predisposition to Disease , NF-kappa B p50 Subunit/genetics , Obesity, Morbid/genetics , Polymorphism, Genetic , Toll-Like Receptor 2/genetics , Adult , Alleles , C-Reactive Protein/immunology , Case-Control Studies , Female , Gene Expression , Gene Frequency , Haplotypes , Humans , Male , Middle Aged , NF-kappa B p50 Subunit/immunology , Obesity, Morbid/diagnosis , Obesity, Morbid/immunology , Odds Ratio , Toll-Like Receptor 2/immunologyABSTRACT
The larvae of Lucilia sericata have been used for centuries as medicinal maggots in the healing of wounds. The present study aimed to screen potential microRNAs related to ES-induced wound healing in rat skin wounds and to investigate the potential mechanisms contributing to accelerated wound healing. Healthy, male, 12 weeks old Wistar albino rats weighing 250-300 g were supplied by the Animal Experimental Center. All animal studies were performed in accordance with the NIH Guide for the Care and Use of Laboratory Animals. Wistar albino rats were treated by ES after post wounding and the differentially expressed miRNAs in wound biopsies were screened by microarray analysis at the end of treatments for 4,7 and 10 days. In addition, bioinformatics approaches were used to identify the potential target genes of differentially expressed miRNAs and the functions of their target genes. We found a significant up-regulation of rno-miR-99a* and rno-mir-877 in response to ES treatment. Further investigation of rno-miR-99a* and rno-mir-877 and their target genes (TGFa, TNF, TAGLN, MAPK1, MMP-9) implicated in present study could provide new insight for an understanding lead to the development of new treatment strategies. The identified miRNAs can be new biomarkers for ES- induced wound healing.
