ABSTRACT
AIM: Comparative study of antibiotics resistance and VNTR-typing of Vibrio cholerae non O1/ non O139 strains, isolated on the territory of Rostov region in 2014. MATERIALS AND METHODS: Antibioticogramms of strains were determined by serial dilution method in dense nutrient medium according to MG 4.2.2495-09 (2009). Pheno-, sero- and VNTR-typing was carried out by conventional-methods. RESULTS: The studied strains belonged to V. cholerae species, did not agglutinate with O1 and O139 sera, were atoxigenic hemolysis-positive, did not contain genes of cholera toxin and toxin-coregulating pili of adhesion, contained genes of hemagglutinin/protease, protease PrtV, collagenase, cytotonic factor Cef, outer membrane protein-OmpW, tol- and -vps-clusters, regulatory genes toxR and hapR. Antibioticogramms of the strains have shown the presence of cultures, resistant to ampicillin, ceftazidime-furazolidone, trimethoprim/sulfamethoxazole with intermediate resistance to streptomycin, kanamycin, gentamycin, amikacin, netilmicin, Approximately 20% of isolates had multiple drug resistance. Data of VNTR- and genotyping confirmed a possibility of water transmission route of the infection. CONCLUSION: Execution of monitoring of cultures from environmental samples is necessary for timely detection of genetic characteristics, antibiotics resistance.
Subject(s)
Cholera/epidemiology , Genes, Bacterial , Vibrio cholerae O139/genetics , Vibrio cholerae non-O1/genetics , Water Microbiology , Anti-Bacterial Agents/pharmacology , Bacterial Outer Membrane Proteins/genetics , Bacterial Outer Membrane Proteins/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Cholera/drug therapy , Cholera/microbiology , Cholera/transmission , Cholera Toxin/genetics , Cholera Toxin/metabolism , Collagenases/genetics , Collagenases/metabolism , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Drug Resistance, Multiple, Bacterial , Epidemiological Monitoring , Fimbriae, Bacterial , Gene Deletion , Humans , Immune Sera/chemistry , Metalloendopeptidases/genetics , Metalloendopeptidases/metabolism , Microbial Sensitivity Tests , Peptide Hydrolases/genetics , Peptide Hydrolases/metabolism , Phylogeny , Russia/epidemiology , Serotyping , Transcription Factors/genetics , Transcription Factors/metabolism , Vibrio cholerae O139/classification , Vibrio cholerae O139/drug effects , Vibrio cholerae O139/isolation & purification , Vibrio cholerae non-O1/classification , Vibrio cholerae non-O1/drug effects , Vibrio cholerae non-O1/isolation & purificationABSTRACT
The optimal conditions of arrangement of direct alternative of flatbed enzyme-linked immunosorbent assay and dot-immunoanalysis with application of monoclonal peroxidase conjugates for express identification of comma bacillus of serogroups O1 and O139 both in hospital and field conditions without device support. The direct technique enzyme-linked immunosorbent assay in flatbed alternative shortens time of analysis up to 70-80 minutes and in case of dot enzyme-linked immunosorbent assay on membrane - up to 70-90 minutes. It is established that in case of analysis in conditions of room temperature (20-25 oC) sensitivity of techniques remains at initial level.
ABSTRACT
Analysis of the antibioticograms of 22 strains of Vibrio cholerae non O1/non O139 serogroups (ctxA- tepA-) isolated from the environment in the Rostov Region in 2011 showed that all the cultures were susceptible to ciprofloxacin, aminoglycosides, ceftriaxone, trimetoprime/sulfamethoxazole and resistant to levomycetin and furazolidone. 32%, 18% and 9% of the isolates were resistant to tetracycline, rifampicin and nalidixic acid respectively. No strains of V. cholerae susceptible to all the tested antimicrobials were detected. 37% of the V. cholerae isolates was resistant to two antibacterials and the others showed multiple resistance and contained 3-6 r-determinants of antibiotic resistance. Since the antibiotic resistance genes in Vibrio cholerae non O1/non O139 serogroups are often located on mobile genetic elements (plasmids, interferons, SXT elements), many strains of such organisms, the same as the natural environment, could serve as reservoirs of antibiotic resistance. The presence of antibiotic resistance r-determinants in the investigated strains in various combinations, the antibiotic resistance variability in the isolates collected on the same territory within a relatively short period of time require monitoring of antibiotic susceptibility in them and the use of the antibiotic for the etiotropic therapy only in strict accordance with the antibioticogram of the culture isolated from the concrete patient.
