ABSTRACT
We reproduced mouse model of bronchial asthma, evaluated metabolic shifts of serum homeostasis during disease development, and compared these results with the data on metabolic shifts in bronchial asthma patients. In experimental mice pronounced pathological changes in the lungs, considerable lymphocytosis and decrease in neutrophil count were detected. Metabolic shifts were studied using method of laser correlation spectroscopy (LCS) of blood serum and urine. Method of LCS allows determining the dispersion composition of the studied fluid by the relative contribution of particle components into light scattering. The relative content of particles of different size.characterizes the direction of metabolic shifts in the organism. Similar patterns of particle distribution in biological fluids of experimental animals and patients suggest that LCS can be used as an additional method for evaluation of the severity of the disease and efficiency of therapy.
Subject(s)
Asthma/blood , Intracellular Space/chemistry , Adult , Animals , Asthma/diagnosis , Asthma/urine , Bronchi/pathology , Case-Control Studies , Female , Humans , Lymphocytes/pathology , Male , Mice , Mice, Inbred C57BL , Middle Aged , Neutrophils/pathologyABSTRACT
This study was designed to investigate the effects of simulated microgravity on the plasma kinetics of ceftriaxone in rats. Comparative analysis of pharmacokinetic parameters in control and experimental groups has revealed the influence of antiorthostatic hypokinesia (ANOH) on the pharmacokinetics of ceftriaxone. Decreased level of maximum plasma concentration (C(max)) and bioavailability of ceftriaxone after single intramuscular (IM) injection were revealed in rats with antiorthostatic hypokinesia. The rate of absorption of the drug was higher respectively.
Subject(s)
Anti-Infective Agents/pharmacokinetics , Ceftriaxone/pharmacokinetics , Weightlessness , Animals , Immobilization , RatsABSTRACT
The method of laser correlation spectroscopy (LCS) was used for the examination of the subfractional compound of tear fluid in patients who have been using soft contact lens (SCL) for a long period and before and after the excimer laser correction with transepithelial photorefractive keratectomy (Trans PRK). Based on the analysis of biological material of 141 patients aged from 18 to 33 years old, we can conclude that prolonged usage of SCL leads to catabolic processes in surface of the anterior segment of the eye and, as a result, to changes in the qualitative composition of the tear fluid. The amount of low molecular weight fractions and medium weight ingredients increases after transepithelial photorefractive keratectomy, which can characterize reparation processes after cornea ablation. Based on the results of our study we can conclude that LCS--is express analysis, with which we can diagnose and observe the initial changes in local metabolism in the anterior segment of the eye, when the objective breaches have not seen yet.
Subject(s)
Contact Lenses, Hydrophilic/adverse effects , Cornea/pathology , Keratectomy, Subepithelial, Laser-Assisted/adverse effects , Photorefractive Keratectomy/adverse effects , Tears/chemistry , Adolescent , Adult , Case-Control Studies , Cornea/surgery , Corneal Injuries , Female , Humans , Lasers, Excimer , Male , Postoperative Complications/diagnosisABSTRACT
We studied radiosensitivity of 101/Hf, C3H/Sn, and C57Bl mice exposed to sublethal doses of γ-radiation. C57Bl mice responded to radiation much later than 101/Hf and C3H/Sn mice, while their adaptability was high enough. 101/Hf and C3H/Sn mice developed acute radiation sickness in a similar way. However, C3H/Sn mice showed better physiological indicators after radiation crisis. There was no noticeable improvement after the development of radiation sickness in 101/Hf mice. To assess individual radiosensitivity, integrated approach should be applied using the data obtained by different methods on several physiological levels.
Subject(s)
Radiation Tolerance , Animals , Dose-Response Relationship, Radiation , Gamma Rays , Mice , Mice, Inbred C57BL , Radiation Injuries, ExperimentalABSTRACT
The effects of TRAIL at various physiological levels were studied on 101/Hf and C3H/Sn mice during acute γ-irradiation with 750 R. An intravenous injection of TRAIL attenuated the effects of γ-irradiation on the body weight, subfractions of blood plasma, horizontal and vertical activity in the open-field test, tissue injury in the small intestine and liver, and content of Hassall's corpuscles in the thymus. The degree of these changes differed in mice of study strains.
Subject(s)
Gamma Rays , Radiation Injuries, Experimental/prevention & control , Radiation-Protective Agents/administration & dosage , TNF-Related Apoptosis-Inducing Ligand/administration & dosage , Animals , Drug Evaluation, Preclinical , Male , Mice, Inbred C3H , Motor Activity/radiation effects , Radiation Tolerance/drug effectsABSTRACT
On the cells of neuroendocrine tumor of rats (line PC12) in culture was estimating of the governing mechanisms of the cytotoxicity of the oxidative stress and the role of the amyloids in increasing this stress. Using flowcytometric assessment of the cytotoxicity H2O2 and fragment beta-amyloid (Abeta) peptide (25-35) has been shown the dose-dependent increasing of the quote of the cells with DNA content < 2c. Isoeffective consentrations were 1 MM H2O2 and 5 MkappaM Abeta. The cytotoxicity H2O2 and A were accompanig with the increasing of the intracellular level of O2-. The treatment of the cells GSNO (donor of NO) and o-phenantrolin (chelators of Fe ions) significantly decreased the intracellular level of O2- as well as the cytotoxicity H2O2 and Abeta. Thus, in direct experiments has been shown of the part amyloids in the increasing of the oxidative stress and participation of the reactive oxide radicals in the cytotoxic effect of the Abeta. The addition argument which confirmed contribution of the oxidative stress in the cytotoxic effect of the Abeta was the similarity of the cellular response on the action of the oxidative agent - H2O2 and Abeta.
Subject(s)
Amyloid beta-Peptides/toxicity , Hydrogen Peroxide/toxicity , Iron/pharmacology , Nitric Oxide/pharmacology , Superoxides/pharmacology , Animals , Apoptosis/drug effects , Cell Survival/drug effects , Oxidation-Reduction , Oxidative Stress , PC12 Cells , RatsABSTRACT
The cytotoxic effects of human blood serum were studied under normal and pathological conditions. The contribution of the complement system to the realization of this effect was demonstrated. Significant differences in the serum capacity to kill foreign cells were detected for healthy subjects and asthmatics. Bovine sperm suspension can serve as the test object for rapid evaluation of functional activity of the complement system.
Subject(s)
Asthma/immunology , Complement System Proteins/pharmacology , Cytotoxins/pharmacology , Serum/immunology , Animals , Athletes , Cattle , Complement System Proteins/immunology , Complement System Proteins/metabolism , Cytotoxins/immunology , Cytotoxins/metabolism , Furosemide , Heparin , Humans , Male , Serum/metabolism , Sperm Motility/drug effectsABSTRACT
Computed tomography images are analyzed in patients with and without lacrimal drainage system abnormalities. Potential of functional multidetector computed tomography in dacryostenosis is showed.
Subject(s)
Lacrimal Apparatus Diseases/diagnosis , Lacrimal Apparatus , Multidetector Computed Tomography/methods , Adult , Aged , Diagnostic Techniques, Ophthalmological , Female , Humans , Lacrimal Apparatus/anatomy & histology , Lacrimal Apparatus/pathology , Lacrimal Apparatus/physiopathology , Lacrimal Apparatus Diseases/pathology , Lacrimal Apparatus Diseases/physiopathology , Male , Middle Aged , Patient Positioning , Patient Selection , Reproducibility of ResultsABSTRACT
Digital radiological images of normal and patients with lacrimal pathways disorders were analyzed. Potentials of digital X-ray in diagnosis of lacrimal pathways disorders are demonstrated.
