ABSTRACT
Immunohistochemical detection of the LYVE-1 marker in healthy human full-thickness skin (the epidermis and the dermis) was carried out. LYVE-1 expression was found in the endothelium of lymphatic capillaries located in the papillary dermis, in the endothelium of larger lymphatic vessels of the reticular dermis, and in fibroblasts, which indicates their joint participation in hyaluronan metabolism. LYVE-1+ staining detected for the first time in cells of the stratum basale, the stratum spinosum, and the stratum granulosum of healthy human epidermis indicates their participation in hyaluronan metabolism and allows us to consider the spaces between epidermis cells as prelimphatics.
Subject(s)
Epidermis , Hyaluronic Acid , Lymphatic Vessels , Skin , Vesicular Transport Proteins , Humans , Hyaluronic Acid/metabolism , Vesicular Transport Proteins/metabolism , Vesicular Transport Proteins/genetics , Skin/metabolism , Lymphatic Vessels/metabolism , Epidermis/metabolism , Ligands , Fibroblasts/metabolism , Dermis/metabolism , Lymphatic System/metabolism , Adult , Female , Male , ImmunohistochemistryABSTRACT
We studied the formation and cytophysiological characteristics of polynuclear macrophages in primary cultures of peritoneal cells from C57Bl/6 mice. Production of reactive oxygen species and phagocytic activity in polynuclear macrophages were higher than in mononuclear macrophages. The formation of polynuclear macrophages in cultures of peritoneal cells is realized via amitosis.
