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Clavulanic acid biosynthesis in Streptomyces clavuligerus: gene cloning and characterization.
Hodgson, J E; Fosberry, A P; Rawlinson, N S; Ross, H N; Neal, R J; Arnell, J C; Earl, A J; Lawlor, E J.
Gene ; 166(1): 49-55, 1995 Dec 01.
Article in English | MEDLINE | ID: mdl-8529893

ABSTRACT

Seven classes of Streptomyces clavuligerus mutants defective in clavulanic acid (CLA) biosynthesis have been identified and used to clone the chromosomal DNA encoding eight CLA biosynthetic genes. The complete sequences of three and the partial sequences of two of these biosynthetic genes are reported, together with their known or predicted functions.


Subject(s)
Anti-Bacterial Agents/biosynthesis , Clavulanic Acids/biosynthesis , Streptococcus/genetics , Acetyltransferases/genetics , Amino Acid Sequence , Base Sequence , Clavulanic Acid , Cloning, Molecular , DNA, Bacterial/genetics , Genes, Bacterial , Genetic Complementation Test , Molecular Sequence Data , Promoter Regions, Genetic , Restriction Mapping , Ureohydrolases/genetics
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