ABSTRACT
Contact-resonance atomic force microscopy (CR-AFM) is of great interest and very valuable for a deeper understanding of the mechanics of biological materials with moduli of at least a few GPa. However, sample surfaces can present a high topography range with significant slopes, where the local angle can be as large as ⯱â¯50°. The non-trivial correlation between surface slope and CR-frequency hinders a straight-forward interpretation of CR-AFM indentation modulus measurements on such samples. We aim to demonstrate the significant influence of the surface slope on the CR-frequency that is caused by the local angle between sample surface and the AFM cantilever and present a practical method to correct the measurements. Based on existing analytical models of the effect of the AFM set-up's intrinsic cantilever tilt on CR-frequencies, we compute the non-linear variation of the first two (eigen)modes CR-frequency for a large range of surface angles. The computations are confirmed by CR-AFM experiments performed on a curved surface. Finally, the model is applied to directly correct contact modulus measurements on a durum wheat starch granule as an exemplary sample.
Subject(s)
Microscopy, Atomic Force/methods , Starch/chemistry , Triticum/chemistry , VibrationABSTRACT
The goal of this paper is to propose an experimental method allowing the identification of the complete elastic tensor of anisotropic biological materials such as wood using only one sample. To do so, two complementary methods are used. First, the wood eigen-directions are defined from a sample of spherical shape that is then cut into a cube in a way to perform resonant ultrasound spectroscopy (RUS). The method is successfully applied on a reference beech sample with known orthotropic directions. A comparison of the identified elastic constants with those from the literature and some inferred from ultrasonic transmission measurements is given.
ABSTRACT
In this work, we propose a study dedicated to the influence of the delay line nature in transverse ultrasonic sensors, dedicated to dynamic high frequency elastic moduli of viscoelastic materials estimation. In literature, these shear ultrasonic rheometers are using delay lines in glass or quartz and normal or oblique incidence of ultrasonic rays. The oblique incidence is used in order to improve the sensitivity of the measurements. We theoretically demonstrate in this work that the use of delay lines in polymers is recommended to improve the sensitivity. Due to modifications, performed on a 10 MHz commercial ultrasonic sensor, we experimentally show on glycerin (which is a Newtonian material) that it is possible to multiply by a factor 10 the sensitivity; compared to delay lines in quartz using a normal incidence of rays. Hence, we overpass the accuracy of the oblique incidence approach with a simpler experimental setup.
