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1.
Development ; 141(8): 1757-66, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24715464

ABSTRACT

Multiple cell types involved in the regulation of angiogenesis express Wnt ligands. Although ß-catenin dependent and independent Wnt signaling pathways have been shown to control angiogenesis, the contribution of individual cell types to activate these downstream pathways in endothelial cells (ECs) during blood vessel formation is still elusive. To investigate the role of ECs in contributing Wnt ligands for regulation of blood vessel formation, we conditionally deleted the Wnt secretion factor Evi in mouse ECs (Evi-ECKO). Evi-ECKO mice showed decreased microvessel density during physiological and pathological angiogenesis in the postnatal retina and in tumors, respectively. The reduced microvessel density resulted from increased vessel regression accompanied by decreased EC survival and proliferation. Concomitantly, survival-related genes were downregulated and cell cycle arrest- and apoptosis-inducing genes were upregulated. EVI silencing in cultured HUVECs showed similar target gene regulation, supporting a mechanism of EC-derived Wnt ligands in controlling EC function. ECs preferentially expressed non-canonical Wnt ligands and canonical target gene expression was unaffected in Evi-ECKO mice. Furthermore, the reduced vascularization of Matrigel plugs in Evi-ECKO mice could be rescued by introduction of non-canonical Wnt5a. Treatment of mouse pups with the non-canonical Wnt inhibitor TNP470 resulted in increased vessel regression accompanied by decreased EC proliferation, thus mimicking the proliferation-dependent Evi-ECKO remodeling phenotype. Taken together, this study identified EC-derived non-canonical Wnt ligands as regulators of EC survival, proliferation and subsequent vascular pruning during developmental and pathological angiogenesis.


Subject(s)
Endothelial Cells/metabolism , Neovascularization, Pathologic/metabolism , Neovascularization, Physiologic , Wnt Proteins/metabolism , Animals , Animals, Newborn , Apoptosis/drug effects , Apoptosis/genetics , Autocrine Communication/drug effects , Autocrine Communication/genetics , Cell Count , Cell Proliferation/drug effects , Cell Survival/drug effects , Cyclohexanes/pharmacology , DNA-Binding Proteins/metabolism , Endothelial Cells/drug effects , Gene Expression Regulation/drug effects , Human Umbilical Vein Endothelial Cells/drug effects , Human Umbilical Vein Endothelial Cells/metabolism , Humans , Ligands , MDS1 and EVI1 Complex Locus Protein , Mice , Mice, Transgenic , Models, Biological , Neovascularization, Pathologic/genetics , Neovascularization, Pathologic/pathology , Neovascularization, Physiologic/drug effects , Neovascularization, Physiologic/genetics , O-(Chloroacetylcarbamoyl)fumagillol , Phenotype , Proto-Oncogenes , Retina/growth & development , Retina/metabolism , Sesquiterpenes/pharmacology , Signal Transduction/drug effects , Signal Transduction/genetics , Transcription Factors/metabolism
2.
J Neurosci ; 30(44): 14835-42, 2010 Nov 03.
Article in English | MEDLINE | ID: mdl-21048142

ABSTRACT

Dicer-dependent noncoding RNAs, including microRNAs (miRNAs), play an important role in a modulation of translation of mRNA transcripts necessary for differentiation in many cell types. In vivo experiments using cell type-specific Dicer1 gene inactivation in neurons showed its essential role for neuronal development and survival. However, little is known about the consequences of a loss of miRNAs in adult, fully differentiated neurons. To address this question, we used an inducible variant of the Cre recombinase (tamoxifen-inducible CreERT2) under control of Camk2a gene regulatory elements. After induction of Dicer1 gene deletion in adult mouse forebrain, we observed a progressive loss of a whole set of brain-specific miRNAs. Animals were tested in a battery of both aversively and appetitively motivated cognitive tasks, such as Morris water maze, IntelliCage system, or trace fear conditioning. Compatible with rather long half-life of miRNAs in hippocampal neurons, we observed an enhancement of memory strength of mutant mice 12 weeks after the Dicer1 gene mutation, before the onset of neurodegenerative process. In acute brain slices, immediately after high-frequency stimulation of the Schaffer collaterals, the efficacy at CA3-to-CA1 synapses was higher in mutant than in control mice, whereas long-term potentiation was comparable between genotypes. This phenotype was reflected at the subcellular and molecular level by the elongated filopodia-like shaped dendritic spines and an increased translation of synaptic plasticity-related proteins, such as BDNF and MMP-9 in mutant animals. The presented work shows miRNAs as key players in the learning and memory process of mammals.


Subject(s)
DEAD-box RNA Helicases/deficiency , Endoribonucleases/deficiency , Gene Deletion , Hippocampus/metabolism , Learning/physiology , Memory/physiology , MicroRNAs/genetics , Animals , DEAD-box RNA Helicases/biosynthesis , DEAD-box RNA Helicases/genetics , Dendritic Spines/metabolism , Dendritic Spines/ultrastructure , Electric Stimulation/methods , Endoribonucleases/biosynthesis , Endoribonucleases/genetics , Hippocampus/ultrastructure , Long-Term Potentiation/genetics , Mice , Mice, Knockout , Mice, Transgenic , MicroRNAs/antagonists & inhibitors , MicroRNAs/biosynthesis , Organ Culture Techniques , Ribonuclease III , Synapses/metabolism , Synapses/ultrastructure
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