Subject(s)
Chickens , Fowlpox/complications , Haemophilus Infections/veterinary , Poultry Diseases , Psittacosis/veterinary , Animals , Conjunctivitis/complications , Conjunctivitis/veterinary , Disease Outbreaks/veterinary , Endophthalmitis/complications , Endophthalmitis/veterinary , Female , Haemophilus Infections/complications , Male , Psittacosis/complicationsABSTRACT
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies in turkey serum to hemorrhagic enteritis virus. The ELISA antigen was extracted from turkey spleens and partially purified with fluorocarbon. Antibodies were demonstrated in serum samples of breeding and meat flocks that had been naturally exposed to infection. These samples were also examined in parallel by agar-gel precipitin (AGP); most of the sera were AGP-positive. ELISA, however, was more sensitive in detecting antibodies in day-old sera that were AGP-negative. The passively acquired antibodies were no longer detected by 4 weeks of age. A brisk but short-lived secondary response was detected by ELISA in the sera of turkeys immunized with beta-propiolactone-inactivated extract of infected spleens.
Subject(s)
Antibodies, Viral/biosynthesis , Coronaviridae/immunology , Coronavirus, Turkey/immunology , Enteritis, Transmissible, of Turkeys/immunology , Gastrointestinal Hemorrhage/veterinary , Turkeys , Animals , Antigens, Viral/isolation & purification , Enzyme-Linked Immunosorbent Assay , Female , Male , Precipitin Tests/veterinary , Spleen/immunology , Vaccination/veterinaryABSTRACT
Two hemagglutinating (HA) agents were isolated from coots (Fulica atra). One of them was isolated from a brain specimen, the specimens being taken from 8 dead birds. The other one was isolated from a tracheal swab, the tracheal and cloacal swabs being taken from 47 apparently healthy birds. Both the agents were identified as Yucaipa-like avian paramyxoviruses (PMV-2) by means of HA inhibition (HI) and neuraminidase (Nase) inhibition (NI) tests. This is the third species of feral birds and the second species of migrating waterfowl in Israel from which Yucaipa-like viruses were isolated.
Subject(s)
Birds/microbiology , Brain/microbiology , Paramyxoviridae/isolation & purification , Trachea/microbiology , Animals , Israel , Paramyxoviridae/classificationABSTRACT
An acute respiratory disease of turkeys in Israel was first reported in November 1978. Alcaligenes faecalis was isolated from sick turkeys and from chickens not affected by the disease. Plate agglutination tests with A. faecalis antigen of 1,067 turkey and 494 chicken serum samples gave variable results: healthy turkeys gave positive reactions and sick turkeys sometimes gave negative ones. All isolated strains were highly sensitive in vitro drug sensitivity tests, but chemotherapy failed in the field. Pathogenicity trials with A. faecalis, given alone or in combination with Yucaipa virus to 8-day-old turkey poults, failed to reproduce the disease.
