Effects of dietary ractopamine hydrochloride and zilpaterol hydrochloride supplementation on performance, carcass traits, and carcass cutability in beef steers.

British × Continental steers (initial BW = 484.6 kg) were fed at a commercial feed yard to evaluate the effects of ß-agonists on live performance, carcass characteristics, and carcass subprimal yield. Weights and ultrasonic measurements were used to allocate steers to pens (n = 40) divided equally into 4 blocks, with 2 treatment replicates per block. Pens were randomly assigned to 1 of 5 treatments: control; ractopamine-HCl (RH) fed at 200 or 300 mg • steer(-1) • d(-1), or 400 mg • steer(-1) • d(-1) top dress for the final 30 d of feeding; or zilpaterol-HCl (ZH) fed at 7.5 mg/kg beginning 23 d before slaughter with a 3-d withdrawal period. Steers were harvested by block at a commercial facility over 4 wk. Carcass based performance measures were calculated using initial pen weights and actual DMI. From each pen, eight carcasses that were within ± 13.6 kg of the mean pen HCW were selected such that two carcasses were within each of the following four Yield Grade (YG) ranges: YG ≤ 2.8; 2.9-3.2; 3.3-3.5; YG > 3.5. Carcasses were fabricated by plant personnel to determine subprimal yield. Steers fed ZH had higher carcass-based ADG and carcass-based G:F compared with all other treatments (P < 0.05). Carcass-based ADG and carcass-based G:F were higher in RH treatments compared with controls (P < 0.05). Steers fed ZH had higher dressing percentages (1.0 to 1.6%) and larger LM area (4.3 to 6.7 cm(2)) than all other treatments (P < 0.05). Use of RH 400 and ZH increased HCW 6.3 and 11.1 kg, respectively compared with controls (P < 0.05). Compared with controls, RH 300 and ZH decreased marbling score and the frequency of carcasses qualifying for upper 2/3 Choice premiums (P < 0.05). Beta-agonists increased subprimal yield from the round and loin; however, blade meat was the only cut from the rib or chuck affected by ß-agonists. Results from this study indicated improvements in performance and carcass traits as a result of ß-agonist use; however, differences between ZH, RH 400, and RH 300 treatments were minimal for carcass traits and cutability. Increases in saleable yield following ß-agonist use were not uniformly distributed across the four major primals and the majority of weight gain occurred in the lower priced cuts of the round and chuck. Increased response of the lower priced cuts to ß-agonists could have economic implications to packers.

A reference-beam autocollimator with nanoradian sensitivity from mHz to kHz and dynamic range of 10(7).

We describe an autocollimating optical angle sensor with a dynamic range of 9 mrad and nrad/√Hz sensitivity at frequencies from 5 mHz to 3 kHz. This work improves the standard multi-slit autocollimator design by adding two optical components, a reference mirror and a condensing lens. This autocollimator makes a differential measurement between a reference mirror and a target mirror, suppressing common-mode noise sources. The condensing lens reduces optical aberrations, increases intensity, and improves image quality. To further improve the stability of the device at low frequencies the body of the autocollimator is designed to reduce temperature variations and their effects. A new data processing technique was developed in order to suppress the effects of imperfections in the CCD.

Effects of ractopamine hydrochloride and zilpaterol hydrochloride supplementation on longissimus muscle shear force and sensory attributes of beef steers.

Effect of ractopamine hydrochloride (RH) and zilpaterol hydrochloride (ZH) on LM shear force and sensory attributes was determined using pens (n = 40) British × Continental crossbred steers randomly allocated to one of the following treatments: control; RH fed at 200 (RH 200) or 300 mg • steer(-1) • d(-1) (RH 300), or 400 mg • steer(-1) • d(-1) (RH 400) top-dressed for the final 30 d of feeding; or ZH fed at 7.5 mg/kg, beginning 23 d before slaughter with a 3-d withdrawal. Two replicates (pens) per treatment were represented in four blocks. Eighteen carcasses per pen were randomly selected and one 5-cm LM sample was removed from both carcass sides to be used for shear force and sensory evaluation. Samples were aged for 14 d, frozen at -28.8 °C, and cut into 2.5-cm steaks. All steaks were cooked to an internal temperature of 71.1 °C before being evaluated for Warner-Bratzler shear force (WBSF), slice shear force (SSF), or being fed to trained sensory panelists. Increasing dose and potency of ß-agonist increased WBSF by 4 to 17% and SSF by 5 to 24% (P < 0.05). Steaks from steers fed ZH had higher WBSF and SSF values compared with all other treatments (P < 0.05), whereas steaks from controls and steers fed RH 200 were not different (P > 0.05). Probability of steaks failing to meet shear force standards to be certified tender (WBSF <4.4 kg, SSF < 20 kg) was increased from an initial probability of <0.06 in steaks from steers in the control treatment to 0.10 to 0.20 in steers fed RH 400 or ZH (P < 0.05). No difference was detected in panel ratings for overall tenderness of steaks from steers fed RH 200 compared with controls (P > 0.05). Steaks from steers fed RH 300 and RH 400 were comparable for all sensory attributes; however, both RH 300 and RH 400 were rated lower for overall tenderness than controls (P < 0.05). Panelists failed to detect differences in overall tenderness of steaks from steers fed RH 400 and ZH (P < 0.05). Panelists detected no difference in flavor profile or juiciness among treatments (P > 0.05). Results from this study indicated ß-agonists negatively affected beef tenderness and these effects may be more noticeable in steers supplemented with ZH and higher doses of RH.

The cerebellum in the cerebro-cerebellar network for the control of eye and hand movements--an fMRI study.

The coordination of optical information and manipulation of objects in space by eye and hand movements is controlled by a cerebro-cerebellar network. The differential influence of prefrontal, motor, or parietal areas in combination with cerebellar areas, especially within the posterior hemispheres, on the control of eye and hand movements is not very well defined. Using fMRI we investigated the functional representation of isolated or combined eye and hand movements within the cerebellum and the impact of differential cognitive preload on the activation patterns. Each task consisted of the performance of saccades or hand movements triggered by a cue presented on a screen in front of the scanner. Saccades were tested for visually guided saccades, triple step saccades, and for visuospatial memory. Sequential finger opposition movements were tested for predictive and nonpredictive movements. Combined and isolated eye-hand reaching movements were tested toward a target presented in 5 different horizontal positions. Visually guided saccades activated the cerebellar vermis lobuli VI-VII, triple step saccades, including visuospatial memorization, in addition the cerebellar hemispheres lobuli VII-VIII. Sequential finger movements and reaching movements activated a cerebellar network consisting of the lobuli IV-VI, the vermis, and the lobuli VII-VIII with broader areas and additional regions especially within the lobus VII for more complex movements. The combined in contrast to the isolated performance of eye and hand movements demonstrated specialized activation foci within the cerebellar vermis and posterior hemispheres. We could demonstrate a differential representation of eye and hand movements within the cerebellum. Additional "cognitive" preload within a given task leads to additional activation of the posterior cerebellar hemispheres, with a subspecialization corresponding to premotor and parietal area connections.