ABSTRACT
The essential oil from Curcuma longa L. was analysed by GC/MS. The major components of the oil were ar-turmerone (33.2%), α -turmerone (23.5%) and ß -turmerone (22.7%). The antifungal activities of the oil were studied with regard to Aspergillus flavus growth inhibition and altered morphology, as preliminary studies indicated that the essential oil from C. longa inhibited Aspergillus flavus Link aflatoxin production. The concentration of essential oil in the culture media ranged from 0.01% to 5.0% v/v, and the concentration of curcumin was 0.01-0.5% v/v. The effects on sporulation, spore viability, and fungal morphology were determined. The essential oil exhibited stronger antifungal activity than curcumin on A. flavus. The essential oil reduced the fungal growth in a concentration-dependent manner. A. flavus growth rate was reduced by C. longa essential oil at 0.10%, and this inhibition effect was more efficient in concentrations above 0.50%. Germination and sporulation were 100% inhibited in 0.5% oil. Scanning electron microscopy (SEM) of A. flavus exposed to oil showed damage to hyphae membranes and conidiophores. Because the fungus is a plant pathogen and aflatoxin producer, C. longa essential oil may be used in the management of host plants.
Subject(s)
Aspergillus flavus/cytology , Aspergillus flavus/growth & development , Curcuma/chemistry , Curcumin/pharmacology , Oils, Volatile/pharmacology , Plant Oils/pharmacology , Apoptosis/drug effects , Apoptosis/physiology , Aspergillus flavus/drug effects , Cell Proliferation/drug effects , Cell Size/drug effects , Dose-Response Relationship, DrugABSTRACT
Aflatoxins are highly toxic, mutagenic, teratogenic and carcinogenic mycotoxins. Consumption of aflatoxin-contaminated food and commodities poses serious hazards to the health of humans and animals. Turmeric, Curcuma longa L., is a native plant of Southeast Asia and has antimicrobial, antioxidant and antifungal properties. This paper reports the antiaflatoxigenic activities of the essential oil of C. longa and curcumin. The medium tests were prepared with the oil of C. longa, and the curcumin standard at concentrations varied from 0.01% to 5.0%. All doses of the essential oil of the plant and the curcumin standard interfered with mycotoxin production. Both the essential oil and curcumin significantly inhibited the production of aflatoxins; the 0.5% level had a greater than 96% inhibitory effect. The levels of aflatoxin B(1) (AFB(1)) production were 1.0 and 42.7 µg/mL, respectively, for the samples treated with the essential oil of C. longa L. and curcumin at a concentration of 0.5%.
Subject(s)
Aflatoxins/biosynthesis , Antifungal Agents/pharmacology , Aspergillus flavus/drug effects , Curcuma/chemistry , Oils, Volatile/pharmacology , Plant Oils/pharmacology , Antifungal Agents/chemistry , Antioxidants/chemistry , Antioxidants/pharmacology , Aspergillus flavus/metabolism , Down-Regulation/drug effects , Oils, Volatile/chemistry , Plant Oils/chemistryABSTRACT
Zearalenone, a mycotoxin produced by fungi of the genus Fusarium, including F. graminearum, triggers reproduction disorders in certain animals and hyperestrogen syndromes in humans. Current research investigates three concentrations of neem oil extract (0.1, 0.25 and 0.5 percent) in reducing the production of zearalenone. Neem oil extract decreased zearalenone amount in the three concentrations but highest inhibition (59.05 percent) occurred at 0.1 percent.
ABSTRACT
Zearalenone, a mycotoxin produced by fungi of the genus Fusarium, including F. graminearum, triggers reproduction disorders in certain animals and hyperestrogen syndromes in humans. Current research investigates three concentrations of neem oil extract (0.1, 0.25 and 0.5%) in reducing the production of zearalenone. Neem oil extract decreased zearalenone amount in the three concentrations but highest inhibition (59.05%) occurred at 0.1%.
ABSTRACT
A Região Sul do Brasil é grande produtora de maçã, sendo 80 por cento destinada ao consumo in natura. As micotoxinas são metabólitos secundários de fungos presentes na cadeia alimentar como contaminantes, causando diversos efeitos toxicológicos e imunológicos. Considerando que a patulina seja uma micotoxina produzida por Penicillium expansum, principal contaminante da maçã propôs-se investigar a ação de extratos aquoso e oleoso de Nim (Azadirachta indica) em maçã artificialmente contaminada. Foram testados dois tipos de extratos: o extrato aquoso obtido de maceração de folhas de Nim nas concentrações de 5, 10, 20 e 30 por cento, e o extrato oleoso comercial (DalNeem®), obtido de sementes de Nim, nas concentrações de 0,125, 0,25, 0,5, 1, 2 e 5 por cento. Os extratos aquosos não alteram a produção da patulina em maçãs contaminadas artificialmente por P. expansum, mas o extrato oleoso obtido das sementes da planta diminuiu acentuadamente a produção de patulina, inclusive em concentrações inferiores a 0,5 por cento.
The South region of Brazil is a major apple producer, where 80 percent is destined to the "in natura" consumption. The mycotoxins are fungal secondary metabolites present as contaminants in the food chain, causing several toxicological and immunological effect. Patulin is a mycotoxin mainly produced by Penicillium expansum and well known as the main contaminant in apples. Due to this contamination, the aim of this work was to access the effect of Neem extract (Azadirachta indica) on artificially contaminated apples. Two types of Neem extracts were tested: the aqueous extracts of Neem leafs at 5, 10, 20 and 30 percent concentrations, and commercial seed oil of Neem (DalNeem®) at 0.125, 0.25, 0.5, 1, 2 and 5 percent in water. Although the addition of Neem aqueous extracts in artificially contaminated apples with P. expansum was unable to affect the patulin production, the Neem seed oil extract at concentrations as lower as 0.5 percent, caused a pronounced diminution over patulin production.
