Early detection and differential serodiagnosis of Mycoplasma hyorhinis and Mycoplasma hyosynoviae infections under experimental conditions.

Mycoplasma hyorhinis (MHR) and Mycoplasma hyosynoviae (MHS) are common opportunistic pathogens in the upper respiratory tract and tonsils of swine. The identification of the specific species involved in clinical cases using conventional diagnostic methods is challenging. Therefore, a recombinant chimeric polypeptide based on the seven known variable lipoproteins (A-G) specific of MHR and a cocktail of surface proteins detergent-extracted from MHS cultures were generated and their suitability as antemortem biomarkers for serodiagnosis of MHR- and MHS-infection were evaluated by ELISA. M. hyorhinis and MHS ELISA performance, evaluated using serum samples collected over a 56-day observation period from pigs inoculated with MHR, MHS, M. hyopneumoniae, M. flocculare, or Friis medium, varied by assay, targeted antibody isotype, and cutoffs. The progressions of MHR and MHS clinical diseases were evaluated in relation to the kinetics of the isotype-specific antibody response in serum and bacterial shedding in oral fluids during the observation period. In pigs inoculated with MHR, bacterial DNA was detected in one or more of the 5 pens at all sampling points throughout the study, IgA was first detected at DPI 7, one week before the first clinical signs, with both IgA and IgG detected in all samples collected after DPI 14. The peak of MHS shedding (DPI 8) coincided with the onset of the clinical signs, with both IgA and IgG detected in all serum samples collected ≥ DPI 14. This study demonstrated, under experimental conditions, that both ELISAs were suitable for early detection of specific antibodies against MHR or MHS. The diagnostic performance of the MHR and MHS ELISAs varied depending on the selected cutoff and the antibody isotype evaluated. The high diagnostic and analytical specificity of the ELISAs was particularly remarkable. This study also provides insights into the infection dynamics of MHR-associated disease and MHS-associated arthritis not previously described.

Outbreak of H5N2 highly pathogenic avian Influenza A virus infection in two commercial layer facilities: lesions and viral antigen distribution.

The largest outbreak of highly pathogenic avian Influenza A virus (HPAIV) infection in U.S. history began in December 2014 resulting in the euthanasia of millions of birds and collateral economic consequences to the U.S. poultry industry. We describe 2 cases of H5N2 HPAIV infection in laying hens in Iowa. Following a sharp increase in mortality with minimal clinical signs, 15 dead birds, from 2 unrelated farms, were submitted to the Iowa State University Veterinary Diagnostic Laboratory. Common lesions included diffuse edema and multifocal hemorrhage of the comb, catarrhal exudate in the oropharynx, and multifocal tracheal hemorrhage. Less common lesions included epicardial petechiae, splenic hemorrhage, and pancreatic necrosis. Influenza A virus nucleoprotein was detected by immunohistochemistry in multiple cell types including ependymal cells, the choroid plexus, neurons, respiratory epithelium and macrophages in the lung, cardiac myocytes, endothelial cells, necrotic foci in the spleen, Kupffer cells in the liver, and necrotic acinar cells in the pancreas. Real-time polymerase chain reaction and sequencing confirmed H5N2 HPAIV with molecular characteristics similar to other contemporary U.S. H5N2 HPAIVs in both cases.

Bacterial probiotics as an aid in the control of Clostridium difficile disease in neonatal pigs.

Although Clostridium difficile infection (CDI) is a common disease in swine, there is a lack of prevention strategies. The objectives of this study were to evaluate: i) the effectiveness of Lactobacillus spp. and ii) non-toxigenic C. difficile (NTCD) as prevention for the development of CDI in piglets. Cesarean-derived piglets (N = 150) were randomly assigned to 6 groups: GROUP 1 - negative control (n = 10); GROUP 2 - NTCD only (n = 13); GROUP 3 - Lactobacillus spp. only (n = 14); GROUP 4 - positive control (challenged with toxigenic C. difficile strain) (n = 35); GROUP 5 - NTCD and challenged with the toxigenic C. difficile strain (n = 34); and GROUP 6 - Lactobacillus spp. and challenged with the toxigenic C. difficile strain (n = 44). Piglets which received NTCD showed lower prevalence of toxin-positive feces, mesocolonic edema, and microscopic lesions compared with positive control piglets. Administration of Lactobacillus spp. did not reveal clear benefits.

Probiotiques bactériens pour faciliter le contrôle de la maladie àClostridium difficilechez les porcelets néonataux. Même si l'infection par Clostridium difficile (ICD) est une maladie commune chez les porcs, il existe une absence de stratégies de prévention. Les objectifs de cette étude consistaient à évaluer: i) l'efficacité de Lactobacillus sp. et de ii) C. difficile non toxinogène (CDNT) comme méthode de prévention contre le développement de l'ICD chez les porcelets. Les porcelets délivrés par césarienne (N = 150) ont été assignés au hasard à 6 groupes: GROUPE 1 ­ groupe témoin négatif (n = 10); GROUPE 2 ­ CDNT seulement (n = 13); GROUPE 3 ­ Lactobacillus sp. seulement (n = 14); GROUPE 4 ­ groupe témoin positif (avec épreuve pour la souche toxinogène de C. difficile) (n = 35); GROUPE 5 ­ CDNT et avec épreuve pour la souche toxinogène de C. difficile (n = 34); et GROUPE 6 ­ Lactobacillus sp. et avec épreuve pour la souche toxinogène de C. difficile (n = 44). Les porcelets ayant reçu CDNT ont affiché une prévalence inférieure de fèces positives pour les toxines, de l'œdème du mésocôlon et de lésions microscopiques comparativement aux porcelets du groupe témoin positif. L'administration de Lactobacillus sp. n'a pas révélé de bienfaits évidents.(Traduit par Isabelle Vallières).

Full-Length Genome Sequence of a Plaque-Cloned Virulent Porcine Epidemic Diarrhea Virus Isolate (USA/Iowa/18984/2013) from a Midwestern U.S. Swine Herd.

Porcine epidemic diarrhea (PED) was recognized in U.S. swine for the first time in early 2013. A plaque-purified PED virus (PEDV) isolate (USA/Iowa/18984/2013) was obtained from a diarrheic piglet. The isolate is genetically close to other previously reported U.S. PEDVs and recent Chinese PEDVs and was virulent when inoculated into neonatal pigs.

Effect of age, dose and antibiotic therapy on the development of Clostridium difficile infection in neonatal piglets.

Piglet diarrhea is associated with increased pre-weaning mortality, poor growth rates, and variation in weight at weaning. Clostridium difficile is a known cause of enteric disease in neonatal piglets, yet risk factors associated with C. difficile infection in piglets are unknown. The objectives of this study were (1) to evaluate the consistency and severity of lesions in piglets challenged with C. difficile at different bacterial doses (DOSAGE experiment), (2) evaluate the use of antibiotics as a contributing risk factor in 1-day-old piglets (ANTIMICROBIAL experiment), and (3) to provide a clinical and histological evaluation of C. difficile infection in 10-day-old piglets (AGE experiment). One hundred and eleven conventional neonatal pigs were snatch farrowed and divided into experimental groups addressing the objectives. In the DOSAGE experiment, 40 1-day-old piglets were sham inoculated or challenged with varying amounts of C. difficile heat shocked spores and euthanized 72 h post infection. Results indicate a clear trend for disease development as bacterial numbers increase. In the ANTIMICROBIAL experiment, 39 1-day-old piglets were challenged and then treated with one of four different antibiotics after 16 h. No significant difference in disease development was found. Thirty-three 10-day-old piglets were given varying doses of C. difficile in the AGE experiment. Disease and lesions were reproduced in 10-day-old piglets. Combined results indicate that C. difficile dosage appears to be an important factor that influences the appearance and severity of lesions, 10-day-old pigs can develop disease associated with C. difficile, and antibiotic administration following inoculation may not be a major contributor for disease in neonatal piglets.