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Increased interaction of proteins in nuclear extract from mouse liver and lung tumors with TATA-containing oligonucleotide.
Lysova, M V; Arshinov, T V; Drachkova, I A; Kobzev, V F; Kaledin, V I; Savinkova, L K.
Bull Exp Biol Med ; 145(1): 93-5, 2008 Jan.
Article in English | MEDLINE | ID: mdl-19024013

ABSTRACT

Increased TATA-binding activity of proteins in nuclear extracts from murine hepatocarcinoma HA-1 and murine Lewis lung adenocarcinoma was demonstrated. The dependence of the amount of formed complexes on protein concentration, displacement of labeled 32P-TATA-containing oligonucleotide by its unlabeled analog, and weak interaction with an oligonucleotide containing damaged TATA box confirm specificity of the formed complexes.


Subject(s)
Liver Neoplasms/metabolism , Lung Neoplasms/metabolism , Oligonucleotides , TATA Box , Tissue Extracts/chemistry , Adenocarcinoma/genetics , Adenocarcinoma/metabolism , Animals , Liver Neoplasms/genetics , Lung Neoplasms/genetics , Mice , Mice, Inbred C57BL , Nuclear Proteins/genetics , Nuclear Proteins/metabolism , Oligonucleotides/genetics , Oligonucleotides/metabolism , TATA-Box Binding Protein/genetics , TATA-Box Binding Protein/metabolism
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