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1.
FEMS Microbiol Rev ; 45(6)2021 11 23.
Article in English | MEDLINE | ID: mdl-34160574

ABSTRACT

The metalloenzyme arginase hydrolyzes l-arginine to produce l-ornithine and urea. In bacteria, arginase has important functions in basic nitrogen metabolism and redistribution, production of the key metabolic precursor l-ornithine, stress resistance and pathogenesis. We describe the regulation and specific functions of the arginase pathway as well as summarize key characteristics of related arginine catabolic pathways. The use of arginase-derived ornithine as a precursor molecule is reviewed. We discuss the biochemical and transcriptional regulation of arginine metabolism, including arginase, with the latter topic focusing on the RocR and AhrC transcriptional regulators in the model organism Bacillus subtilis. Finally, we consider similarities and contrasts in the structure and catalytic mechanism of the arginases from Bacillus caldovelox and Helicobacter pylori. The overall aim of this review is to provide a panorama of the diversity of physiological functions, regulation and biochemical features of arginases in a variety of bacterial species.


Subject(s)
Arginase , Helicobacter pylori , Arginase/genetics , Bacillus subtilis/genetics , Bacterial Proteins/genetics , Helicobacter pylori/genetics , Ornithine
2.
Microbiology (Reading) ; 166(3): 278-287, 2020 03.
Article in English | MEDLINE | ID: mdl-31935179

ABSTRACT

In nitrogen-fixing rhizobia, emerging evidence shows significant roles for polyamines in growth and abiotic stress resistance. In this work we show that a polyamine-deficient ornithine decarboxylase null mutant (odc2) derived from Sinorhizobium meliloti Rm8530 had significant phenotypic differences from the wild-type, including greatly reduced production of exopolysaccharides (EPS; ostensibly both succinoglycan and galactoglucan), increased sensitivity to oxidative stress and decreased swimming motility. The introduction of the odc2 gene borne on a plasmid into the odc2 mutant restored wild-type phenotypes for EPS production, growth under oxidative stress and swimming. The production of calcofluor-binding EPS (succinoglycan) by the odc2 mutant was also completely or mostly restored in the presence of exogenous spermidine (Spd), norspermidine (NSpd) or spermine (Spm). The odc2 mutant formed about 25 % more biofilm than the wild-type, and its ability to form biofilm was significantly inhibited by exogenous Spd, NSpd or Spm. The odc2 mutant formed a less efficient symbiosis with alfalfa, resulting in plants with significantly less biomass and height, more nodules but less nodule biomass, and 25 % less nitrogen-fixing activity. Exogenously supplied Put was not able to revert these phenotypes and caused a similar increase in plant height and dry weight in uninoculated plants and in those inoculated with the wild-type or odc2 mutant. We discuss ways in which polyamines might affect the phenotypes of the odc2 mutant.


Subject(s)
Medicago sativa/microbiology , Ornithine Decarboxylase/genetics , Polyamines/metabolism , Root Nodules, Plant , Sinorhizobium meliloti/genetics , Bacterial Proteins/genetics , Medicago sativa/growth & development , Medicago sativa/metabolism , Mutation , Nitrogen/metabolism , Phenotype , Polysaccharides, Bacterial/metabolism , Root Nodules, Plant/metabolism , Root Nodules, Plant/microbiology , Sinorhizobium meliloti/metabolism
3.
Clin Sci (Lond) ; 128(9): 609-18, 2015 May.
Article in English | MEDLINE | ID: mdl-25442240

ABSTRACT

Iron overload (IO) has been associated with glucose metabolism alterations and increased risk of cardiovascular disease (CVD). Primary IO is associated with mutations in the HFE gene. To which extent HFE gene mutations and metabolic alterations contribute to the presence of atherogenic lipoprotein modifications in primary IO remains undetermined. The present study aimed to assess small, dense low-density lipoprotein (LDL) levels, chemical composition of LDL and high-density lipoprotein (HDL) particles, and HDL functionality in IO patients. Eighteen male patients with primary IO and 16 sex- and age-matched controls were recruited. HFE mutations (C282Y, H63D and S65C), measures of insulin sensitivity and secretion (calculated from the oral glucose tolerance test), chemical composition and distribution profile of LDL and HDL subfractions (isolated by gradient density ultracentrifugation) and HDL functionality (as cholesterol efflux and antioxidative activity) were studied. IO patients compared with controls exhibited insulin resistance (HOMA-IR (homoeostasis model assessment-estimated insulin resistance): +93%, P< 0.001). Metabolic profiles differed across HFE genotypes. C282Y homozygotes (n=7) presented a reduced ß-cell function and insulin secretion compared with non-C282Y patients (n=11) (-58% and -73%, respectively, P< 0.05). In addition, C282Y homozygotes featured a predominance of large, buoyant LDL particles (C282Y: 43±5; non-C282Y: 25±8; controls: 32±7%; P< 0.001), whereas non-C282Y patients presented higher amounts of small, dense LDL (C282Y: 23±5; non-C282Y: 39±10; controls: 26±4%; P< 0.01). HDL particles were altered in C282Y homozygotes. However, HDL functionality was conserved. In conclusion, metabolic alterations and HFE gene mutations are involved in the presence of atherogenic lipoprotein modifications in primary IO. To what extent such alterations could account for an increase in CVD risk remains to be determined.


Subject(s)
Atherosclerosis/etiology , Blood Glucose/metabolism , Cholesterol, HDL/blood , Histocompatibility Antigens Class I/genetics , Insulin/blood , Iron Overload/blood , Iron Overload/genetics , Membrane Proteins/genetics , Mutation , Adult , Aged , Atherosclerosis/blood , Atherosclerosis/genetics , Biomarkers/blood , Case-Control Studies , Cell Line , Cholesterol, LDL/blood , DNA Mutational Analysis , Genetic Predisposition to Disease , Glucose Tolerance Test , Hemochromatosis Protein , Heterozygote , Homozygote , Humans , Insulin Resistance , Insulin-Secreting Cells/metabolism , Iron Overload/complications , Iron Overload/diagnosis , Male , Middle Aged , Phenotype , Risk Factors
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