ABSTRACT
The fact that antibiotics exert no effect on Mycobacterium tuberculosis may be attributable to the production of their persistent forms. A correlation was studied between the expression of the genes Rv3286c, Rv2626c, Rv2031, and Rv3133c and the phenotypical tolerance to antibiotics in relation to the physiological condition of M. bovis BCG. The anaerobic growth conditions ("Wayne dormancy model") were found to be attended by the increased expression of all the studied genes, by the higher tolerance to rifampicin, and by the decreased phenotypical resistance to metronidazole.
Subject(s)
Antibiotics, Antitubercular/pharmacology , Drug Resistance, Bacterial/genetics , Gene Expression/physiology , Genes, Bacterial/genetics , Metronidazole/pharmacology , Mycobacterium bovis/genetics , Rifampin/pharmacology , Animals , Colony Count, Microbial , DNA, Bacterial/genetics , Gene Expression/drug effects , Genes, Bacterial/drug effects , In Vitro Techniques , Mycobacterium bovis/drug effects , Mycobacterium bovis/growth & development , Phenotype , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
The type of human papilloma virus (HPV) was determined in 26 children aged between 1 year 10 months to 15 years 5 months suffering from recurrent respiratory papillomatosis (RRP). Polymerase chain reaction identified DNA of HPV type 6, 11, 16 and 18. HPV DNA was detected in all the patients including fifteen patients infected with HPV type 11; seven patients infected with HPV type 6; four children infected with HPV type 6 and 11. Types 16 and 18 of HPV were not detected. The analysis of RRP course has found that laryngeal papillomatosis runs a more aggressive course in cases with HPV type 11 infection than in those with HPV type 6.
Subject(s)
Laryngeal Neoplasms/pathology , Laryngeal Neoplasms/virology , Papilloma/pathology , Papilloma/virology , Papillomaviridae/isolation & purification , Adolescent , Child , Child, Preschool , DNA, Viral , Female , Humans , Infant , Laryngeal Neoplasms/epidemiology , Male , Papilloma/epidemiology , Papillomaviridae/classification , Papillomaviridae/genetics , Polymerase Chain Reaction , Prevalence , RecurrenceABSTRACT
Six Rickettsia sibirica strains isolated in Siberia and Far East (Primorje) from various sources (patient, ticks D. nuttali, D. silvarum, H. concinna) at different time (1940-1980) were studied by the RFLP and DNA probe hybridization techniques. All studied strains were found to have the identical profiles of migrating fragments in restrictograms got by using a set of endonucleases (EcoRI, PstI, PvuII, Bg1I, XbaI, HindIII, MspI) and similar zones of hybridization with a DNA probe derived from Rickettsia prowazekii DNA. The obtained data point to a close similarity between the genomes of investigated Rickettsia sibirica strains. Long-term isolation of the genetically similar Rickettsia sibirica strains testifies to their constant circulation, thus apparently determining the stability of epidemiologic manifestation of tick-borne typhus fever of Northern Asia in the central part of its area (Siberia, Far East).
Subject(s)
Rickettsia/genetics , DNA Probes , Genes, Bacterial , Genotype , Nucleic Acid Hybridization , Polymorphism, Restriction Fragment Length , Species SpecificityABSTRACT
The restriction analysis of 6 Rickettsia prowazekii strains with the use of 8 restrictases (Cfr13I, EcoRI, HindIII, MSpI, MvaI, PstI, XhoI, BamHI) has been carried out. In the presence of considerable homology in the restriction pictures of DNA in these strains some differences in 1-2 fragments within the range of 8,000-20,000 nucleotide pairs have been established. The strains under study have been divided into two groups according to the character of differences in their restrictograms: the group of virulent typing strain Breinl (Breinl, G. Anan'ev) and the group of strain E with low pathogenicity (E, EVir, Katsinian). Differences in the restrictograms of DNA do not correlate with the virulence of R. prowazekii strains and the areas of their isolation.
Subject(s)
DNA, Bacterial/genetics , Genes, Bacterial/genetics , Rickettsia prowazekii/genetics , DNA Restriction Enzymes/pharmacology , DNA, Bacterial/drug effects , DNA, Bacterial/isolation & purification , Genes, Bacterial/drug effects , Restriction Mapping , Rickettsia prowazekii/drug effects , Rickettsia prowazekii/pathogenicity , Virulence/drug effects , Virulence/geneticsABSTRACT
The pIB52 plasmid contains the ribosomal rp1A, J, K, L and RNA-polymerase genes rpo C, B of Escherichia coli. No homology has been found between the plasmid used as a molecular probe and the chromosomal DNA from Rickettsia provazekii in the blot hybridization experiments.
Subject(s)
DNA, Bacterial/genetics , Escherichia coli/genetics , Genes, Bacterial , Rickettsia prowazekii/genetics , Cloning, Molecular , DNA Restriction Enzymes , DNA, Ribosomal/genetics , DNA-Directed RNA Polymerases/genetics , Deoxyribonuclease BamHI , Nucleic Acid Hybridization , Plasmids , Rickettsia prowazekii/immunology , Rickettsial Vaccines , Species SpecificityABSTRACT
The DNA of Rickettsia provazekii strain E was cleaved by PstI restriction endonuclease under the conditions of partial restriction. The fragments were inserted into the PstI site of pBR325 and cloned in this plasmid. E. coli strain HB101 was used as a recipient for cloning. 880 clones sensitive to ampicillin and resistant to tetracycline were selected from 5120 transformants. The cloning of rickettsial DNA has been confirmed by the blot hybridization technique. Analysis of individual and net probes of the hybrid DNA by gel electrophoresis makes it possible to conclude that 90% of the selected clones harbour hybrid plasmids, the size of the cloned fragments rangers from 0.9 to 10.4 Kb, the obtained library of clones contains 70% of the whole genome of Rickettsia provazekii.
