ABSTRACT
The complex pollution of aquifers by reduced and oxidized nitrogen compounds is currently considered one of the urgent environmental problems that require non-standard solutions. This work was a laboratory-scale trial to show the feasibility of using various mineral carriers to create a permeable in situ barrier in cold (10 °C) aquifers with extremely high nitrogen pollution and inhabited by the Candidatus Scalindua-dominated indigenous anammox community. It has been established that for the removal of ammonium and nitrite in situ due to the predominant contribution of the anammox process, quartz, kaolin clays of the Kantatsky and Kamalinsky deposits, bentonite clay of the Berezovsky deposit, and zeolite of the Kholinsky deposit can be used as components of the permeable barrier. Biofouling of natural loams from a contaminated aquifer can also occur under favorable conditions. It has been suggested that the anammox activity is determined by a number of factors, including the presence of the essential trace elements in the carrier and the surface morphology. However, one of the most important factors is competition with other microbial groups that can develop on the surface of the carrier at a faster rate. For this reason, carriers with a high specific surface area and containing the necessary microelements were overgrown with the most rapidly growing microorganisms. Bioaugmentation with a "warm" anammox community from a laboratory reactor dominated by Ca. Kuenenia improved nitrogen removal rates and biofilm formation on most of the mineral carriers, including bentonite clay of the Dinozavrovoye deposit, as well as loamy rock and zeolite-containing tripoli, in addition to carriers that perform best with the indigenous anammox community. The feasibility of coupled partial denitrification-anammox and the adaptation of a "warm" anammox community to low temperatures and hazardous components contained in polluted groundwater prior to bioaugmentation should be the scope of future research to enhance the anammox process in cold, nitrate-rich aquifers.
ABSTRACT
Laboratory modeling of Cs, Sr, U, Pu immobilization by phytoplankton of the river Upa, affected after the Chernobyl accident, has been carried out. Certain conditions are selected for strong fixation of radionuclides in bottom sediments due to biogeochemical processes. The process of radionuclide removal from the water phase via precipitation was based on their accumulation by phytoplankton, stimulated by nitrogen and phosphorus sources. After eight days of stimulation, planktonic phototrophic biomass, dominated by cyanobacteria of the genus Planktothrix, appears in the water sample. The effectiveness of U, Pu and Sr purification via their transfer to bottom sediment was observed within one month. The addition of ammonium sulfate and phosphate (Ammophos) led to the activation of sulfate- and iron-reducing bacteria of the genera Desulfobacterota, Desulfotomaculum, Desulfosporomusa, Desulfosporosinus, Thermodesulfobium, Thiomonas, Thiobacillus, Sulfuritallea, Pseudomonas, which form sulphide ferrous precipitates such as pyrite, wurtzite, hydrotroillite, etc., in anaerobic bottom sediments. The biogenic mineral composition of the sediments obtained under laboratory conditions was verified via thermodynamic modeling.
ABSTRACT
Nickel is one of the most dangerous environmental pollutants and its removal from wastewater is an important task. The capacity of a mineral-organic hybrid adsorbent, consisting of Shewanella xiamenensis biofilm and zeolite (clinoptilolite of the Chola deposit), to remove metal ions from nickel-containing batch systems under different experimental conditions was tested. The obtained biosorbent was characterized using neutron activation, SEM, and FTIR techniques. It was established that maximum removal of cations, up to 100%, was achieved at pH 6.0. Several mathematical models were applied to describe the equilibrium and kinetics data. The maximum adsorption capacity of the hybrid biosorbent, calculated using the Langmuir model, varied from 3.6 to 3.9 mg/g. Negative Gibbs energy values and positive ∆H° values indicate the spontaneous and endothermic character of the biosorption process. The effects of several parameters (pH and biosorbent dosage) on Ni(II) removal from real effluent, containing nickel with a concentration of 125 mg/L, were investigated. The optimal pH for Ni(II) removal was 5.0-6.0 and an increase of sorbent dosage from 0.5 to 2.0 led to an increase in Ni(II) removal from 17% to 27%. At two times effluent dilution, maximum Ni(II) removal of 26% was attained at pH 6.0 and sorbent dosage of 1.0 g. A 12-fold effluent dilution resulted in the removal of 72% of Ni(II) at the same pH and sorbent dosage values. The obtained hybrid biosorbent can be used for Ni(II) removal from industrial effluents with low Ni(II) concentrations.
