ABSTRACT
Materials on the development of an enzyme immunoassay (EIA) system for the detection of the antigens of C. burnetii, the causative agent of Q rickettsiosis, are presented. The system is highly specific and effective with respect to both corpuscular antigens of phases 1 and 2 and soluble antigen (lipopolysaccharide). The sensitivity of this method varies within the range 5-100 ng/ml. The effectiveness of EIA as a quantitative (semiquantitative) control test used in the process of the production of Coxiella preparations has been demonstrated.
Subject(s)
Antigens, Bacterial/analysis , Coxiella/immunology , Animals , Antibodies, Bacterial/isolation & purification , Antigens, Bacterial/immunology , Chick Embryo , Evaluation Studies as Topic , Immune Sera/isolation & purification , Immunization , Immunoenzyme Techniques/instrumentation , Immunoglobulin G/isolation & purification , Mice , Rabbits , SolubilityABSTRACT
The work deals with the development of the rapid method of the identification of acute streptococcal infection on the basis of the coagglutination test. The rapid method of the extraction of group-specific polysaccharide antigen from the cell walls of group A streptococci is proposed. The data on the use of native sera and their fractions in the development of coagglutination diagnostica have been described and analyzed. The advantages of the new method of the diagnosis of acute streptococcal infection in comparison with the traditional microbiological method are shown.
Subject(s)
Streptococcal Infections/diagnosis , Streptococcus pyogenes , Acute Disease , Agglutination Tests/instrumentation , Agglutination Tests/methods , Antibody Specificity/immunology , Antigens/immunology , Antigens/isolation & purification , Antigens, Bacterial/immunology , Antigens, Bacterial/isolation & purification , Cross Reactions/immunology , Epitopes/immunology , Epitopes/isolation & purification , Humans , Immunoglobulin G/analysis , Polysaccharides, Bacterial/immunology , Polysaccharides, Bacterial/isolation & purification , Reagent Kits, Diagnostic , Streptococcus pyogenes/immunology , Time FactorsABSTRACT
Based on the antigen derived from the whole Opisthorchis marita extraction in cats and A-peroxidase protein conjugate the authors modified the enzyme immunoassay system for the detection of IgG antibodies to the causative agent of opisthorchiasis. A vibroshaker used in all stages of study permitted them to reduce the time of the reaction and its analysis providing the visualized consideration of the results.
Subject(s)
Opisthorchiasis/diagnosis , Animals , Antigens, Helminth/isolation & purification , Humans , Immunoenzyme Techniques , Opisthorchis/immunology , Serologic Tests/methods , Staphylococcal Protein AABSTRACT
A method for detection of antituberculous antibodies by enzyme immunoassay system developed by the authors, was tested on 317 blood serum samples taken from patients with tuberculosis and non-tuberculous diseases of the lungs, vertebral column, kidneys and genitalia. Depending on the process site, the detection rate of specific antibodies and their specificity made up 57-77% and 85-95%, respectively, which practically agrees with the data produced as a result of a simultaneously conduction of three routine serologic reactions.
Subject(s)
Antibodies, Bacterial/analysis , Female Urogenital Diseases/diagnosis , Lung Diseases/diagnosis , Male Urogenital Diseases , Mycobacterium tuberculosis/immunology , Spinal Diseases/diagnosis , Tuberculosis, Pulmonary/diagnosis , Tuberculosis, Spinal/diagnosis , Tuberculosis, Urogenital/diagnosis , Diagnosis, Differential , Humans , Immunoenzyme Techniques , Tuberculosis, Pulmonary/immunology , Tuberculosis, Spinal/immunology , Tuberculosis, Urogenital/immunologyABSTRACT
The results of using enzyme immunoassay and latex preparations for the diagnosis of rotavirus gastroenteritis are presented. High effectiveness of the enzyme immunoassay system developed in the USSR with latex diagnostic agents, such as Rotalex (Orion Diagnostica, Finland), Slidex Rota Kit (BioMérieux, France), The Wellcome Rotavirus Latex Kit (Wellcome Foundation Ltd., Great Britain), 48-63% and 21-41% respectively, has been noted. The results of the comparison of the system developed in the USSR with Wellcozyme Rotavirus, an enzyme immunoassay system manufactured by Wellcome Foundation Ltd. (Great Britain), are practically comparable. The results of the block test and the confirmation test used for control indicate that the Soviet preparation is specific. Materials on the practical evaluation of the assay system at health institutions are presented. Good prospects for the use of this system in the diagnosis of rotavirus gastroenteritis, as well as in the realization of epidemiological surveillance on this infection, are substantiated.
Subject(s)
Antigens, Viral/analysis , Immunoenzyme Techniques/instrumentation , Latex Fixation Tests/instrumentation , Reagent Kits, Diagnostic , Rotavirus/immunology , Animals , Cattle , Evaluation Studies as Topic , Feces/microbiology , Gastroenteritis/diagnosis , Gastroenteritis/microbiology , Humans , Rotavirus/isolation & purification , Rotavirus Infections/diagnosis , Rotavirus Infections/microbiology , Swine/microbiologySubject(s)
Gastroenteritis/diagnosis , Rotavirus Infections/diagnosis , Antigens, Viral/analysis , Child , Counterimmunoelectrophoresis/methods , Evaluation Studies as Topic , Feces/microbiology , Humans , Immunoenzyme Techniques , Precipitin Tests/methods , Rotavirus/immunology , Rotavirus/isolation & purificationABSTRACT
A blastolysin fraction, isolated from lysosyme lysates of Lactobacillus Bulgaricus strain 51 by gel chromatography, was shown to markedly inhibit the growth of sarcoma S-180. A single administration of the agent was followed by an insignificant decrease in tumor growth rate, while repeated treatment induced a complete regression of tumor in 15% of animals and resulted in a prolonged suppression of tumor growth in the others. Cells isolated from metastases which developed after the withdrawal of the drug retained susceptibility to fraction 2 of blastolysin. The latter fraction showed a similar pattern of inhibition of the growth of Ehrlich ascites tumor but did not affect hemocytoblastosis in mice LA. Histologically, fraction 2 produced tumor cell necrosis which commenced at 24 hours posttreatment. Such signs of hypercorticoidism as lysis of lymphocytes in the cortical layer of the thymus, depletion of T-dependent zones of spleen and lymph nodes and adrenal cortical hyperplasia were registered. Macrophage level in tumor was not increased. A 7-fold rise in murine serum corticosterone level at 1 hour posttreatment was established by the method of competitive protein binding.
