[Study of interaction of polystirolsulphonate with polymerization degree of 8 and polyallylamin with bilayer lipids membranes].

Interaction of polystirolsulphonate with polymerization degree of 8 (PSS-8) and polyallylamin PAA (molecular mass 60 kilodaltons) with viruses from bloodline of paramixo- and orthomixoviruses by the example of measles virus, parotitis and flu leads to the decreasing of infective activity. The possible mechanism of viral inhibitive action of these chemical compounds is damaging of interfacial antigenic proteins of paramixo- and orthomixoviruses. In this study it was detected the change of surface tension of bilayer lipid membrane in the presence of PSS-8 and PAA. The change of surface tension leads to disorder in viral proteins adsorption in bilayer lipid membrane. This process could lead to disorder of juncture and self-assembly of virions.

[Investigation of antiviral activity of adamantan boron derivaties on pandemic influenza virus models].

Comparative investigation of the virus-inhibiting activity of some boron-containing compounds showed that products BG 12 and BG 4 had the highest inhibitory effect on pandemic viruses. The minimum inhibitory concentration (MIC) of the products was 0.1 mcg/ml. The use of liposomes loaded with BG 12 molecules in the optimal concentration (0.1 mcg/ml) resulted in inhibition of the avian plague virus growth in the MDCK cells. Possible design of efficient drugs for antiviral protection based on the complexes liposomes--boron-containing compounds is discussed.

[Influence of polyelectrolytes on measles virus infectivity].

AIM: Mechanism of virus inhibiting action against measles virus of polyelectrolytes (PE) polystyrolsulfonate (PSS) of various polymerization degree and 60 kDa molecular weight polyallylamine (PAA) was studied. MATERIALS AND METHODS: Measles virus Leningrad-16 strain was used for the study. Virus infectious titer reduction kinetics after interaction with PSS with the degree of polymerization of 8 (PSS 8), 31, 77, 170, 360, 430 and PAA were determined by titration method with cytopathogenic effect detection in Vero continuous cell line. Circular dichroism and fluorescence spectra of viral proteins were obtained by using Zenith 200st spectrophotometer (Russian Federation) and Jasco J-810 dichrograph (Japan). RESULTS: A significant decrease of measles virus infectious titers after interaction with PSS with the degree of polymerization of 8 and PAA in concentration of 30 mM was detected. Analysis of circular dichroism spectra and protein fluorescence allowed to determine the mechanism of interaction of the indicated PE with measles virus surface proteins. The secondary structure of viral proteins is damaged by hydrophobic polar frame of these PE, polyanion PSS 8 also interacts with positive charges of protein groups that leads to the formation of loops and tails that disrupt alpha-spirals. CONCLUSION: The studied PE could be considered as potential antiviral preparations, and methods of circular dichroism and protein fluorescence could be used to detect damage of viral protein secondary structure by agents of different kinds.

[A study of the interaction of boraadamantane with liposomes].

Changes in the surface potential of liposomes obtained from dipalmitoyl phospatidylcholine during their interaction with the new antiviral preparation boraadmantane have been studied. It has been concluded that the saturation of the lipid bilayer of liposomes by boraadmantane occurs at concentrations of the preparation above 10 microg/ml.